Co3O4/PAn Magnetic Nanoparticle-Modified Electrochemical Immunosensor for Azocyclotin

In the current study, for rapid detection of azocylotin residues in agricultural products, the Co3O4/PAn nanoparticles modified electrochemical immunosensor was generated successfully. Azocylotin-BSA artificial antigen coupling to the surface of the working electrode coated with the Co3O4/PAn nanoparticles thin-layer, the competitive inhibition reaction is launched between the azocylotin in the samples and the azocylotin coupled on the electrode with the azocylotin monoclonal antibodies in the test system. The antigen-antibody reaction signal conductive amplified by the coupled silver nanoparticles, and then the electrolytic current in the reaction system was detected. After establishing basic detection system, a series of optimization including the concentration of immobilized membrane optimization, electrode surface coated material composition optimization, selection of buffer, coupling antigen concentration optimization and anti-antibody label optimization will be done. Subsequently, the concentrations of azocylotin in samples of standards, orange and apple were tested, and the results indicated that this immune sensor has good sensitivity and high accuracy. The research could provide the reference for the research, development and application of sensors to realize rapid detection of pesticide residues in agricultural products.

Effect of different Current density on Properties of Electrodeposited Ni-Co-Cr Thin Films

NiCoCr thin films were electroplated for different current density. FCC structured crystals were observed in XRD study. Coercive force and magnetization value exhibits soft magnetic nature. Electroplated NiCoCr films with electrolytic current density (2, 3, 4 and 5 mil.Amp/cm2) shows uniform deposition on the substrate. Cobalt composition was minimum as 21.31 wt% for current density 5 mA/cm2. The consistence of chromium and nickel increased while current density was improved. Thin films deposition with high current density shows low hysteresis loss and soft magnetic nature.The hardness of deposits increases when current density is increased.

Detecting DNA Methylation using the Oxford Nanopore Technologies MinION sequencer

Nanopore sequencing instruments measure the change in electric current caused by DNA transiting through the pore. In experimental and prototype nanopore sequencing devices it has been shown that the electrolytic current signals are sensitive to base modifications, such as 5-methylcytosine. Here we quantify the strength of this effect for the Oxford Nanopore Technologies MinION sequencer. Using synthetically methylated DNA we are able to train a hidden Markov model to distinguish 5-methylcytosine from unmethylated cytosine in DNA. We demonstrate by sequencing natural human DNA, without any special library preparation, that global patterns of methylation can be detected from low-coverage sequencing and that the methylation status of CpG islands can be reliably predicted from single MinION reads. Our trained model and prediction software is open source and freely available to the community under the MIT license.

Organic Sonoelectrochemistry on Mercury Pool Electrode

So far, the influence of sonication on the electrolytic current was studied only at solid or rather miniaturized mercury electrodes. The presented paper reports on sonoelectrochemical experiments at a liquid mercury pool electrode. Two sonoelectrochemical cells have been developed and tested. It was shown that during sonication, the electrolytic current increases in a number of individual peaks representing short local enhancements of current density due to vigorous local mass transfer and instantaneous increase of fresh electrode surface. Both these effects are caused by microjets of solution formed during violent unsymmetric collapses of cavitation bubbles in the close vicinity of the electrode surface. The newly formed electrode surface and the decrease in the diffusion layer thickness were estimated and discussed. An example is presented where the sonication is used for destruction of a film of products formed during electrolysis of cysteine, that otherwise rapidly inhibits continuation of the electrode process.