Marine soundscape and fish biophony of a Mediterranean marine protected area

Background Marine soundscape is the aggregation of sound sources known as geophony, biophony, and anthrophony. The soundscape analysis, in terms of collection and analysis of acoustic signals, has been proposed as a tool to evaluate the specific features of ecological assemblages and to estimate their acoustic variability over space and time. This study aimed to characterise the Capo Caccia-Isola Piana Marine Protected Area (Italy, Western Mediterranean Sea) soundscape over short temporal (few days) and spatial scales (few km) and to quantify the main anthropogenic and biological components, with a focus on fish biophonies. Methods Within the MPA, three sites were chosen each in a different protection zone (A for the integral protection, B as the partial protection, and C as the general protection). In each site, two underwater autonomous acoustic recorders were deployed in July 2020 at a depth of about 10 m on rocky bottoms. To characterise the contribution of both biophonies and anthrophonies, sea ambient noise (SAN) levels were measured as sound pressure level (SPL dB re: 1 μ Pa-rms) at eight 1/3 octave bands, centred from 125 Hz to 16 kHz, and biological and anthropogenic sounds were noted. Fish sounds were classified and counted following a catalogue of known fish sounds from the Mediterranean Sea based on the acoustic characteristic of sound types. A contemporary fish visual census had been carried out at the test sites. Results SPL were different by site, time (day vs. night), and hour. SPLs bands centred at 125, 250, and 500 Hz were significantly higher in the daytime, due to the high number of boats per minute whose noise dominated the soundscapes. The loudest man-made noise was found in the A zone, followed by the B and the C zone, confirming that MPA current regulations do not provide protection from acoustic pollution. The dominant biological components of the MPA soundscape were the impulsive sounds generated by some invertebrates, snapping shrimps and fish. The vast majority of fish sounds were recorded at the MPA site characterized by the highest sound richness, abundance, and Shannon-Wiener index, coherently with the results of a fish visual census. Moreover, the acoustic monitoring detected a sound associated with a cryptic species (Ophidion spp.) never reported in the study area before, further demonstrating the usefulness of passive acoustic monitoring as a complementary technique to species census. This study provides baseline data to detect future changes of the marine soundscapes and some suggestions to reduce the impact of noise on marine biodiversity.

A primer on rhythm quantification for fish sounds: a Mediterranean case study

We have used a lately established workflow to quantify rhythms of three fish sound types recorded in different areas of the Mediterranean Sea. So far, the temporal structure of fish sound sequences has only been described qualitatively. Here, we propose a standardized approach to quantify them, opening the path for assessment and comparison of an often underestimated but potentially critical aspect of fish sounds. Our approach is based on the analysis of inter-onset-intervals (IOIs), the intervals between the start of one sound element and the next. We calculate exact beats of a sequence using Fourier analysis and IOI analysis. Furthermore, we report on important parameters describing the variability in timing within a given sound sequence. Datasets were chosen to depict different possible rhythmic properties: Sciaena umbra sounds have a simple isochronous—metronome-like—rhythm. The /Kwa/ sound type emitted by Scorpaena spp. has a more complex rhythm, still presenting an underlying isochronous pattern. Calls of Ophidion rochei males present no rhythm, but a random temporal succession of sounds. This approach holds great potential for shedding light on important aspects of fish bioacoustics. Applications span from the characterization of specific behaviours to the potential discrimination of yet not distinguishable species.

A Fish Chorus on the Margin of New Jersey Atlantic Continental Shelf

We report herein an underwater biological chorus coming from the margin of the New Jersey Atlantic continental shelf that we tentatively attribute to a species of fish. The chorus occurred every night for over a month during the Shallow Water 2006 experiment and covers the frequency band 150–4,800 Hz, with maximum intensity in the band from 1450 to 2,000 Hz. Remarkable intensity peaks occurred at 500, 725, 960, 1,215, 1,465, 1,700, and 1,920 Hz, rising to as much as 20 dB above the background noise without the chorus. The chorus begins at sunset and reaches its maximum intensity within an hour, following which it weakens slightly and then gradually climbs again to a peak before sunrise, at which point it quickly weakens and disappears. Its frequency-domain characteristics and the nocturnal timing are reminiscent of sound produced by underwater animals. The intensity of the chorus weakens along the across-shelf path going shoreward, which indicates that the chorus originates from the margin of the continental shelf rather than from the coastal zone, as is generally considered. The chorus contains a single type of acoustic signal that takes the form of double-pulse bursts that last about 8.7 ms, with each pulse containing several acoustic cycles. The time interval between successive bursts varies from 1.5 to 1.9 s. Signals containing a number of bursts vary in length from tens to hundreds of seconds. Although it is impossible to determine the fish species responsible for the chorus, its characteristics, including its low frequency and intensity, its single type of short-duration sound signal, and its multiple peaks in the frequency domain, are all consistent with the general characteristics of fish sounds.

The Use of Soundscapes to Monitor Fish Communities: Meaningful Graphical Representations Differ with Acoustic Environment

Many marine animals produce sounds in several phases of their life cycles, either actively or as a byproduct of their activities, such as during mate attraction or when moving. Recent studies of underwater soundscapes have proved passive acoustic monitoring to be a cost-effective, non-invasive tool to understand ecological processes, especially when sampling in adverse conditions or at great depth. Four days of sound recordings at three seamounts from the Azorean archipelago were examined to assess the suitability of different sound graphical representations to characterize different acoustic environments that contrast in the contribution of vocal fish communities. Long-term spectrograms, sound pressure level, spectral probability densities and the Acoustic Complexity Index (ACI) were computed for two shallow seamounts (Formigas and Princesa Alice, c. 35 m) and one deep seamount (Condor, 190 m) using graphics with different time spans. Only in Formigas, which presented the highest occurrence of fish sounds, was it possible to observe temporal patterns of fish vocal activity in the graphical representations. We highlight that habitats with a higher diversity and abundance of sounds are the most suitable targets for these methods, while in locations with a low prevalence of fish sounds a combination of several methods would be recommended.

Drivers of male sound production and effective communication distances at fish spawning aggregation sites

While monitoring fish sounds has enhanced our understanding of spatio-temporal patterns of spawning and acoustic communication, data interpretation often fails to account for environmental effects on acoustic recordings, resulting in uncertainty of whether measures of detected fish sounds correspond to rates of sound production, specific behaviours, and abundance. In this study, we applied acoustic propagation modelling and detection theory to estimate rates of sound production of territorial, male Gulf grouper (Mycteroperca jordani) from passive acoustic recordings and evaluate effective communication distances. To assess behavioural drivers, environmentally calibrated, hourly estimates of sound production rates were compared to diver observations of courtship, spawning, and numbers of females encountered within male territories. Rates of sound production increased before sunset and were correlated to observed rates of spawning and females encountered, indicating that sound production is largely driven by female presence and increased opportunities to spawn. The mean effective communication distance was estimated to be <21 m, supporting the importance of short-range communication within the observed behaviours. Our findings corroborate that fish sounds can be used to infer measures of reproductive activity and the relative abundance of both sexes during spawning periods once properly calibrated for environmental effects and detection capabilities.

Characterization of the acoustic community of vocal fishes in the Azores

Sounds produced by teleost fishes are an important component of marine soundscapes, making passive acoustic monitoring (PAM) an effective way to map the presence of vocal fishes with a minimal impact on ecosystems. Based on a literature review, we list the known soniferous fish species occurring in Azorean waters and compile their sounds. We also describe new fish sounds recorded in Azores seamounts. From the literature, we identified 20 vocal fish species present in Azores. We analysed long-term acoustic recordings carried out since 2008 in Condor and Princesa Alice seamounts and describe 20 new putative fish sound sequences. Although we propose candidates as the source of some vocalizations, this study puts into evidence the myriad of fish sounds lacking species identification. In addition to identifying new sound sequences, we provide the first marine fish sound library for Azores. Our acoustic library will allow to monitor soniferous fish species for conservation and management purposes.