<p>Soils are the largest stock of terrestrial carbon, the dynamics of soil organic C (SOC) are controlled by microbial physiology, but how it promotes stable SOC and how it would change with warming, remains unknown. The Huascar&#225;n National Park (HNP), the largest mass of tropical glaciers in the world, has lost 20-30% of its glacial cover and the temperatures in this biosphere have risen 0.1&#176;C per decade since 1970. However, no information on the HNP soil carbon stocks is available. As managing SOC is important for global warming mitigation, we study the soil C stocks in Polylepis forests of three valleys in the HNP along a temperature gradient relative to elevation (3300 to 4500 m asl), and their vulnerability to decomposition with increasing temperatures and combined labile C and nutrient (N+P) additions.</p><p>We found that higher altitude soils have higher C:N:P ratios which indicates that, as expected, soils at high altitudes are nutrient limited. Also, the activities of the N acquiring enzymes: NAGase and leucine-aminopeptidase, C acquiring enzymes: beta-glucosidase, cellobiosidase, beta-xylosidase and phosphatase were positively correlated with altitude, which indicate that N and P availability decreased with altitude across our gradient. This could make high altitude soils vulnerable to C losses, not just due to increased temperatures, but also due to increased rhizosphere priming effects. Climate warming might increase plant growth and belowground C allocation, which in turn could lead to priming due to nutrient mining.</p><p>We found no differences across altitudes in microbial biomass (Cmic) measured with the chloroform fumigation extraction method. We are currently analysing microbial community composition (by PLFA&#8217;s and DNA based methods). We will present data on microbial CUE of glucose decomposition, and how it is related to soil C/N ratios, nutrient availability and nutrient requirements, and community composition of the microbes. We also aim to test whether higher CUE is related to higher C stabilisation potential in the form of microbial necromass residues (amino sugars), or higher C loss when microbes efficiently growing on labile substrates will also increase the decomposition of more stable SOC (priming).</p>