Proteomic identification of ruminal epithelial protein expression profiles in response to starter feed supplementation in pre-weaned lambs

Sodium fluoride (NaF) is an anion that has been previously shown to block the moulting process ofAscaris suumlarvae. This study describes moulting and development-specific protein expression profiles ofA. suumlung-stage L3 (AsLL3) following NaF exposure. AsLL3s cultured in the presence or absence of NaF were prepared for protein analysis using two-dimensional (2D) electrophoresis. NaF exposure inhibited at least 22 proteins in AsLL3 compared with moulted larvae (i.e. AsLL4). A further comparison of AsLL4 with those of pre-cultured AsLL3 and NaF-exposed AsLL3 revealed 8 stage-specifically and 4 over-expressed proteins. Immunoblot analysis revealed an inhibition by NaF of 19 immunoreactive proteins. Enzyme assay and immunochemical data showed an inhibition of the moulting-specific inorganic pyrophosphatase activity by 41% and a decreased expression in NaF-treated larvae, indicating its significance in the moulting process. A protein spot associated with NaF inhibition was isolated and identified by peptide mass spectrometry and bioinformatics approaches to be a member of 3–hydroxyacyl–CoA dehydrogenase/short-chain dehydrogenase enzyme families. These results have implications for the identification of proteins specific to the moulting process as potential chemotherapeutic targets.

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ProteinChip Technology Reveals Distinctive Protein Expression Profiles in the Urine of Bladder Cancer Patients

European Urology ◽

10.1016/j.eururo.2005.02.016 ◽

2005 ◽

Vol 47 (6) ◽

pp. 885-894 ◽

Cited By ~ 35

Author(s):

J. Mueller ◽

F. von Eggeling ◽

D. Driesch ◽

J. Schubert ◽

C. Melle ◽

...

Keyword(s):

Bladder Cancer ◽

Protein Expression ◽

Cancer Patients ◽

Expression Profiles ◽

Protein Expression Profiles

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PATH-29. GLIOBLASTOMA TUMOR CLASSIFICATION BASED ON SPATIAL ANALYSIS OF ONCOGENIC AMPLIFICATIONS AND PROTEIN EXPRESSION

Neuro-Oncology ◽

10.1093/neuonc/noab196.481 ◽

2021 ◽

Vol 23 (Supplement_6) ◽

pp. vi121-vi121

Author(s):

Kacper Walentynowicz ◽

Dalit Engelhardt ◽

Shreya Yadav ◽

Ugoma Onubogu ◽

Roberto Salatino ◽

...

Keyword(s):

Tumor Microenvironment ◽

Protein Expression ◽

Single Cell ◽

Expression Profiles ◽

Protein Profiling ◽

Intratumor Heterogeneity ◽

Neuronal Markers ◽

Associated Proteins ◽

Dual Amplification ◽

Protein Expression Profiles

Abstract Heterogeneity of glioblastoma (GBM) has been extensively studied in recent years with identification of oncogenic drivers of GBM cellular subtypes. However, little is known about how these cells interact with each other or with the surrounding tumor microenvironment (TME). We employed spatial protein profiling targeting immune and neuronal markers (79 proteins) coupled with single-cell spatial maps of fluorescence in situ hybridization (FISH) for EGFR, CDK4, and PDGFRA on human GBM tissue sections. Several cores from 20 GBM samples were collected to create a tissue microarray, and 96 regions of interests were profiled with 37,844 nuclei for oncogenic amplification screen. Spatial protein profiling identified strong correlation of certain immune markers, TAU-associated proteins, and oligodendrocyte-enriched protein groups and overall high intratumor heterogeneity of TME. Our single-cell quantification of FISH signals showed differences among tumors based on the prevalence of dual amplification of EGFR and CDK4 within a cell relative to single oncogene amplified cells. High relative frequency of dual amplification was associated with increased expression of immune-related markers and decreased expression of EGFR protein. Moreover, this protein expression signature was associated with survival in another GBM dataset. Here, we present spatial genetic analysis at the single cell level coupled with protein expression profiles associated with tumor microenvironment. Our results suggest that assessment of genetic heterogeneity in GBM could potentially drive improved patient stratification and treatment.

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Upregulation of PKC genes and isozymes in cardiovascular tissues during early stages of experimental diabetes

Physiological Genomics ◽

10.1152/physiolgenomics.00125.2002 ◽

2003 ◽

Vol 12 (2) ◽

pp. 139-146 ◽

Cited By ~ 49

Author(s):

Mingzhang Guo ◽

Mack H. Wu ◽

Ferenc Korompai ◽

Sarah Y. Yuan

Keyword(s):

Protein Expression ◽

Cardiovascular System ◽

Time Course ◽

Experimental Diabetes ◽

Pathological Condition ◽

Expression Profiles ◽

Diabetic Patients ◽

Mrna Levels ◽

Early Stages ◽

Protein Expression Profiles

The protein kinase C (PKC) pathway has recently been recognized as an important mechanism in the development of diabetic complications including cardiomyopathy and angiopathy. Although an increase in PKC kinase activity has been detected in the cardiovascular system of diabetic patients and animals, it is unclear whether the same pathological condition alters PKC at the transcriptional and translational levels. In this study we assessed quantitatively the mRNA and protein expression profiles of PKC isozymes in the heart and vascular tissues from streptozotocin-induced diabetic pigs. Partial regions of the porcine PKCα, β1, and β2 mRNAs were sequenced, and real-time RT-PCR assays were developed for PKC mRNA quantification. The results showed a significant increase in the mRNA levels of PKCα, β1, and β2 in the heart at 4–8 wk of diabetes. In concomitance, the PKCβ1 and β2 genes, but not the PKCα gene, were upregulated in the diabetic aorta. Correspondingly, there was a significant increase in the protein expression of PKCα and β2 in the heart and PKCβ2 in the aorta with a time course correlated to that of mRNA expression. In summary, PKCβ2 was significantly upregulated in the heart and aorta at both the transcriptional and translational levels during early stages of experimental diabetes, suggesting that PKCβ2 may be a prominent target of diabetic injury in the cardiovascular system.

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