Nutrient Dynamics Assessment of Coarse Wood Debris Subjected to Successional Decay Levels of Three Forests Types in Northeast, China

Coarse wood debris (CWD) plays a critical role in forest productivity, nutrient cycling, decomposition, and carbon sequestration, and shapes the carbon pool in the forest ecosystem. However, the elemental composition of CWD varies among different forest types and decay classes for the same dominant tree species (Pinus koraiensis, PK). We compared CWD elemental composition across different forest types (Picea koraiensis-Abies nephrolepis-Pinus koraiensis forest (PAPF), Betula costata-Pinus koraiensis forest (BPF), Tilia amurensis-Pinus koraiensis forest (TPF)), considering four classes of wood decay. Results showed that N, P, Mg, Mn, Na, Zn, S, Al, and Fe concentrations almost totally increased with decay level for all three forest types, except for K in all three forest types and B in Picea koraiensis-Abies nephrolepis-Pinus koraiensis forest (PAPF). Similarly, maximum concentrations of N, P, B, Mg, K, C, Zn, and Mn of CWD were observed in Betula costata-Pinus koraiensis forest (BPF) under varying decay classes, but their maximum concentrations of Fe and S were found in Picea koraiensis-Abies nephrolepis-Pinus koraiensis forest (PAPF) and Tilia amurensis-Pinus koraiensis forest(TPF), respectively. Only C content did not significantly differ in decay classes across all three forest types. The C:N ratio decreased significantly with increasing decay levels across all forest types. The decay rates were significantly related to N concentration and C:N ratio in decay classes across all forest types. These results suggest that C and N concentration are the key factors affecting its decomposition. The variation in nutrient concentrations observed here underscores the complexity of nutrients stored in wood debris in forested ecosystems.

Comparative transcriptome analysis during developmental stages of direct somatic embryogenesis in Tilia amurensis Rupr

Tilia species are valuable woody species due to their beautiful shape and role as honey trees. Somatic embryogenesis can be an alternative method for mass propagation of T. amurensis. However, the molecular mechanisms of T. amurensis somatic embryogenesis are yet to be known. Here, we conducted comparative transcriptional analysis during somatic embryogenesis of T. amurensis. RNA-Seq identified 1505 differentially expressed genes, including developmental regulatory genes. Auxin related genes such as YUC, AUX/IAA and ARF and signal transduction pathway related genes including LEA and SERK were differentially regulated during somatic embryogenesis. Also, B3 domain family (LEC2, FUS3), VAL and PKL, the regulatory transcription factors, were differentially expressed by somatic embryo developmental stages. Our results could provide plausible pathway of signaling somatic embryogenesis of T. amurensis, and serve an important resource for further studies in direct somatic embryogenesis in woody plants.

Divergences in hydraulic conductance and anatomical traits of stems and leaves in three temperate tree species coping with drought, N addition and their interactions

Drought and nitrogen (N) addition have been shown to affect tree hydraulic traits, but few studies have been made on their interactions across species with different wood types or leaf forms. We examined the responses of hydraulic conductance and xylem anatomical traits of Quercus mongolica (ring porous with simple leaves), Fraxinus mandshurica (ring porous with compound leaves) and Tilia amurensis (diffuse porous with simple leaves) to drought, N addition and their interactions. Drought stress decreased current-year xylem-specific conductivity in stems (Ksx) and leaf hydraulic conductance (Kleaf ), but N addition affected Ksx and Kleaf differently among species and watering regimes. These divergent effects were associated with different responses of anatomical traits and leaf forms. Higher mean vessel diameter in stems and lower vessel density in leaves were observed with N addition. The three-way interactive effects of drought, N addition and tree species were significant for most values of anatomical traits. These results were also reflected in large differences in vessel diameter and density among species with different wood types or leaf forms. The two-way interactive effects of drought and N addition were significant on Kleaf and predawn water potential, but not Ksx, indicating that leaves were more sensitive than stems to a combination of drought stress and N addition. Our results provide mechanistic insight into the variable responses of xylem water transport to the interactions of drought and N availability.

Impact of leaf retained water on tree transpiration

Water retained on tree leaves after rainfall, dew, or fog impacts transpiration. To determine the impact of leaf retained water (LRW) on the transpiration of trees, the difference between transpiration rates of different species with and without water sprayed on the leaves was measured. Results show that transpiration was inhibited by LRW. Both the inhibition extent and duration of LRW were higher on broadleaf species than on coniferous species. Under conditions of saturated LRW of the tree crown, mean transpiration inhibition rates of the test species were 0.32 (Quercus mongolica Fisch. ex Ledeb.), 0.25 (Acer mono), 0.37 (Tilia amurensis Rupr.), 0.31 (Fraxinus mandshurica Rupr.), 0.22 (Pinus koraiensis Siebold & Zucc.), 0.22 (Abies nephrolepis (Trautv. ex Maxim.) Maxim.), and 0.23 (Picea asperata Mast.). Mean inhibition rate and inhibition time for broadleaf species were 0.31 and 115 min, which were 40% higher and 27 min longer than those for coniferous species, respectively. The transpiration inhibition rate of LRW increased linearly with the LRW amount, and there was a higher transpiration inhibition rate in broadleaf species than in coniferous species for the same amount of LRW. Mean rising velocity of the inhibition with LRW amount for broadleaf species was 0.53%, whereas it was only 0.15% for the coniferous species.