PSXI-29 Effects of feed restriction on blood constituent concentrations in hair sheep

Forty-six Dorper, 47 Katahdin, and 41 St. Croix female sheep (initial body weight of 62, 62, and 51 kg, respectively, SEM=1.43; 3.8±0.18 yr of age) from 45 commercial farms in Midwest, Northwest, Southeast, and central Texas regions of the United States were used to evaluate effects of feed restriction on concentrations of blood constituents. A 50% concentrate pelleted diet was fed, with the amount varied in the first 4 wk to achieve stable BW. The amount of feed offered in wk 5–10 was set at 55% of that consumed in wk 3–4. Blood was sampled at the end of wk 3, 4, 6, 8, and 10, with constituent levels in wk 4 and 10 assumed relevant to conditions with different maintenance energy requirements (i.e., fed at maintenance and approximately 43% lower with restricted intake). There were some differences among breeds such as ones based on samples collected at all times in urea nitrogen (14.0, 13.7, and 15.4 mg/dl; SEM=0.31) and creatinine (0.945, 0.836, and 0.809 mg/dl for Dorper, Katahdin, and St. Croix, respectively; SEM=0.0253) but relatively few among regions and only one interaction between week and breed or region. There was a trend for a difference (P = 0.051) between wk 4 and 10 in the concentration of glucose (51.9 and 54.2 mg/dl; SEM=0.90), and there were differences (P < 0.05) in levels of lactate (23.9 and 20.3 mg/dl; SEM=0.89), urea N (16.4 and 13.0 mg/dl; SEM= 0.25), creatinine (0.808 and 0.919 mg/dl; SEM=0.0165), triglycerides (31.8 and 25.5 mg/dl; SEM=0.63), cholesterol (67.5 and 74.7 mg/dl; SEM=1.66), and cortisol (10.55 and 8.31 ng/ml for wk 4 and 10, respectively; SEM=0.0542). In conclusion, similar responses of different hair sheep breeds in blood constituent levels to feed restriction is in accordance with comparable effects on body weight and the maintenance energy requirement previously reported.

Study the effects of naturally acquired canine Dirofil Ariasis on some hematological and biochemical parameters

The present study was aimed to check the amount of variation in some hematologic and biochemical parameters accompanied with natural acquired canine dirofilariasis. Blood samples were collected from sixty five stray dogs (5-10) years old belong to local breed dog in the villages of Al-Hindya area/ Karbala Governorate, The affected animal showed differences in hematological and biochemical values as compared with reference ranges. In conclusion, the disease showed no significant clinical signs although many pathological changes in some blood constituent with serum biochemical parameters were observed, before euthanasia for dogs.

Plasma fibronectin stabilizes Borrelia burgdorferi–endothelial interactions under vascular shear stress by a catch-bond mechanism

Bacterial dissemination via the cardiovascular system is the most common cause of infection mortality. A key step in dissemination is bacterial interaction with endothelia lining blood vessels, which is physically challenging because of the shear stress generated by blood flow. Association of host cells such as leukocytes and platelets with endothelia under vascular shear stress requires mechanically specialized interaction mechanisms, including force-strengthened catch bonds. However, the biomechanical mechanisms supporting vascular interactions of most bacterial pathogens are undefined. Fibronectin (Fn), a ubiquitous host molecule targeted by many pathogens, promotes vascular interactions of the Lyme disease spirochete Borrelia burgdorferi. Here, we investigated how B. burgdorferi exploits Fn to interact with endothelia under physiological shear stress, using recently developed live cell imaging and particle-tracking methods for studying bacterial–endothelial interaction biomechanics. We found that B. burgdorferi does not primarily target insoluble matrix Fn deposited on endothelial surfaces but, instead, recruits and induces polymerization of soluble plasma Fn (pFn), an abundant protein in blood plasma that is normally soluble and nonadhesive. Under physiological shear stress, caps of polymerized pFn at bacterial poles formed part of mechanically loaded adhesion complexes, and pFn strengthened and stabilized interactions by a catch-bond mechanism. These results show that B. burgdorferi can transform a ubiquitous but normally nonadhesive blood constituent to increase the efficiency, strength, and stability of bacterial interactions with vascular surfaces. Similar mechanisms may promote dissemination of other Fn-binding pathogens.

A New, Non-Invasive in vivo Optical Blood Glucose Measurement Technique Using Near-Infrared Radiation (“Pulse Glucometry”) and a Proposal for “Pulse Hemo-Photometry” Blood Constituent Measurements

A recently proposed optical method for a non-invasive in vivo blood glucose level (BGL) measurement named “pulse glucometry” is introduced. This method is based on near-infrared living body spectroscopy to accurately obtain blood information. The remarkable feature of the method is the measurement of both the total transmitted radiation spectra in wavelength ? (I?) and the cardiac-related pulsatile component (?I?). When ?I? is superimposed on I?, the differential optical density (?OD?), which includes only arterial blood information, is obtained, thus avoiding interference from living tissues other than arterial blood. Another feature is the ability to measure the differential optical density (?OD?) in multiple wavelengths to avoid interference from blood constituents other than the target blood chemical (glucose). To support this methodology, a very fast near-infrared spectroscopic system was developed to obtain a photoplethysmographic cardiac signal with a resolution of 8 nm over a wavelength range of 900 to 1700 nm at a 100 Hz sampling frequency. An example of an in vivo BGL measurement is shown and indicates good prediction capabilities. This method can be expanded to the measurement of other blood constituents.