Improving Oil Recovery of the Heterogeneous Low Permeability Reservoirs by Combination of Polymer Hydrolysis Polyacrylamide and Two Highly Biosurfactant-Producing Bacteria

Polymer hydrolysis polyacrylamide and microbes have been used to enhance oil recovery in many oil reservoirs. However, the application of this two-method combination was less investigated, especially in low permeability reservoirs. In this work, two bacteria, a rhamnolipid-producing Pseudomonas aeruginosa 8D and a lipopeptide-producing Bacillus subtilis S4, were used together with hydrolysis poly-acrylamide in a low permeability heterogeneous core physical model. The results showed that when the two bacterial fermentation liquids were used at a ratio by volumeof 1:3 (v:v), the mixture showed the optimal physicochemical properties for oil-displacement. In addition, the mixture was stable under the conditions of various temperature (20–70 °C) and salinity (0–22%). When the polymer and bacteria were mixed together, it had no significant effects in the viscosity of polymer hydrolysis polyacrylamide and the viability of bacteria. The core oil-displacement test displayed that polymer hydrolysis polyacrylamide addition followed by the bacterial mixture injection could significantly enhance oil recovery. The recovery rate was increased by 15.01% and 10.03%, respectively, compared with the sole polymer hydrolysis polyacrylamide flooding and microbial flooding. Taken together, these results suggest that the strategy of polymer hydrolysis poly-acrylamide addition followed by microbial flooding is beneficial for improving oil recovery in heterogeneous low permeability reservoirs.

Numerical Investigation on Low Salinity Augmented Microbial Flooding LSAMF within a Sandstone Core for Enhanced Oil Recovery Under Nonisothermal and Fluctuating pH Conditions

Summary In an era of increasing energy demand, declining oil fields and fluctuating crude oil prices globally, most oil companies are looking forward to implementing cost effective and environmentally sustainable enhanced oil recovery (EOR) techniques such as low salinity waterflooding (LSWF) and microbial EOR (MEOR). The present study numerically investigates the combined influence of simultaneous LSWF and microbial flooding for in-Situ MEOR in tertiary mode within a sandstone core under spatiotemporally fluctuating pH and temperature conditions. The developed black oil model consists of five major coupled submodels: nonlinear heat transport model; ion transport coupled with multiple ion exchange (MIE) involving uncomplexed cations and anions; pH variation with salinity and temperature; coupled reactive transport of injected substrates, Pseudomonas putida and produced biosurfactants with microbial maximum specific growth rate varying with temperature, salinity and pH; relative permeability and fractional flow curve variations due to interfacial tension reduction and wettability alteration (WA) by LSWF and biofilm deposition. The governing equations are solved using finite difference technique. Operator splitting and bisection methods are adopted to solve the MIE-transport model. The present model is found to be numerically stable and agree well with previously published experimental and analytical results. In the proposed MIE-transport mechanism, decreasing injection water salinity (IWS) from 2.52 to 0.32 M causes enhanced Ca2+ desorption rendering rock surface towards more water wet. Consequently, oil relative permeability (kro) increases with >55% reduction in water fractional flow (fw) at water saturation of 0.5 from the initial oil-wet condition. Further reducing IWS to 0.03 M causes Ca2+ adsorption shifting the surface wettability towards more oil-wet thus increasing fw by 52%. Formation water salinity (FWS) showed minor impact on WA with <5% decrease in fw when FWS is reduced from 3.15 to 1.05 M. During LSAMF, biosurfactant production is enhanced by >63% on reducing IWS from 2.52 to 0.32 M with negligible increase on further reducing IWS and FWS. This might be due to limiting nonisothermal (40 to 55 °C) and nutrient availability conditions. LSAMF caused significant WA, increase in kro with fw reduction by >84%. Though pH increased from 8.0 to 8.9, it showed minor impact on microbial metabolism. Formation damage due to bioplugging observed near injection point is compensated by effective migration of biosurfactants deep within sandstone core. The present study is a novel attempt to show synergistic effect of LSAMF over LSWF in enhancing oil mobility and recovery at core scale by simultaneously addressing complex crude oil-rock-brine chemistry and critical thermodynamic parameters that govern MEOR efficiency within a typical sandstone formation. The present model with relatively lower computational cost and running time improves the predictive capability to pre-select potential field candidates for successful LSAMF implementation.

Isolating, identifying and evaluating of oil degradation strains for the air-assisted microbial enhanced oil recovery process

Due to the inefficient reproduction of microorganisms in oxygen-deprived environments of the reservoir, the applications of microbial enhanced oil recovery (MEOR) are restricted. To overcome this problem, a new type of air-assisted MEOR process was investigated. Three compounding oil degradation strains were screened using biochemical experiments. Their performances in bacterial suspensions with different amounts of dissolved oxygen were evaluated. Water flooding, microbial flooding and air-assisted microbial flooding core flow experiments were carried out. Carbon distribution curve of biodegraded oil with different oxygen concentration was determined by chromatographic analysis. The long-chain alkanes are degraded by microorganisms. A simulation model was established to take into account the change in oxygen concentration in the reservoir. The results showed that the optimal dissolved oxygen concentration for microbial growth was 4.5~5.5mg/L. The main oxygen consumption in the reservoir happened in the stationary and declining phases of the microbial growth systems. In order to reduce the oxygen concentration to a safe level, the minimum radius of oxygen consumption was found to be about 145m. These results demonstrate that the air-assisted MEOR process can overcome the shortcomings of traditional microbial flooding techniques. The findings of this study can help for better understanding of microbial enhanced oil recovery and improving the efficiency of microbial oil displacement.

Viral Abundance and Diversity of Production Fluids in Oil Reservoirs

Viruses are widely distributed in various ecosystems and have important impacts on microbial evolution, community structure and function and nutrient cycling in the environment. Viral abundance, diversity and distribution are important for a better understanding of ecosystem functioning and have often been investigated in marine, soil, and other environments. Though microbes have proven useful in oil recovery under extreme conditions, little is known about virus community dynamics in such systems. In this study, injection water and production fluids were sampled in two blocks of the Daqing oilfield limited company where water flooding and microbial flooding were continuously used to improve oil recovery. Virus-like particles (VLPs) and bacteria in these samples were extracted and enumerated with epifluorescence microscopy, and viromes of these samples were also sequenced with Illumina Hiseq PE150. The results showed that a large number of viruses existed in the oil reservoir, and VLPs abundance of production wells was 3.9 ± 0.7 × 108 mL−1 and virus to bacteria ratio (VBR) was 6.6 ± 1.1 during water flooding. Compared with water flooding, the production wells of microbial flooding had relative lower VLPs abundance (3.3 ± 0.3 × 108 mL−1) but higher VBR (7.9 ± 2.2). Assembled viral contigs were mapped to an in-house virus reference data separate from the GenBank non-redundant nucleotide (NT) database, and the sequences annotated as virus accounted for 35.34 and 55.04% of total sequences in samples of water flooding and microbial flooding, respectively. In water flooding, 7 and 6 viral families were identified in the injection and production wells, respectively. In microbial flooding, 6 viral families were identified in the injection and production wells. The total number of identified viral species in the injection well was higher than that in the production wells for both water flooding and microbial flooding. The Shannon diversity index was higher in the production well of water flooding than in the production well of microbial flooding. These results show that viruses are very abundant and diverse in the oil reservoir’s ecosystem, and future efforts are needed to reveal the potential function of viral communities in this extreme environment.

Experimental study of microbial enhanced oil recovery in oil-wet fractured porous media

Without Enhanced Oil Recovery (EOR) operations, the final recovery factor of most hydrocarbon reservoirs would be limited. However, EOR can be an expensive task, especially for methods involving gas injection. On the other hand, aqueous injection in fractured reservoirs with small oil-wet or mixed-wet matrices will not be beneficial if the rock wettability is not changed effectively. In the current research, an unpracticed fabrication method was implemented to build natively oil-wet, fractured micromodels. Then, the efficiency of microbial flooding in the micromodels, as a low-cost EOR method, is investigated using a new-found bacteria, Bacillus persicus. Bacillus persicus improves the sweep efficiency via reduction of water/oil IFT and oil viscosity, in-situ gas production, and wettability alteration mechanisms. In our experiments, the microbial flooding technique extracted 65% of matrix oil, while no oil was produced from the matrix system by water or surfactant flooding.