Synergic coordination of stem cells is required to induce a regenerative response in anthozoan cnidarians

Little is known about the origin of the inductive signal that translates the amputation stress into a cooperative cellular response. By studying the process underlying the reformation of lost body parts in the anthozoan cnidarian Nematostella vectensis, we identified a regeneration-inducing structure that, via a tissue crosstalk, is responsible for the initiation of the repair program. We further revealed for the first time in anthozoan cnidarians, that fast and slow-cycling/quiescent stem cells respond to the amputation stress and actively participate in the reformation of lost body parts. Importantly, a synergic interaction of both stem cell populations is required to complete the regeneration process. Our findings suggest that the emergence/loss of structure complexity/compartmentalization influences the proprieties of tissue plasticity, changes the competence of a tissue to reprogram and, in the context of regeneration, the capacity of the tissue to emit or respond to a regeneration-inducing signal.

The Effects of Position on the Wear Debris Detection with Planar Inductor

Wear debris detection is an effective method to determine the running state of the machine. Recently, the planar inductor is commonly used to detect wear debris. The previous studies have found that the inductive signal would be varied while changing the position of wear debris pass through. However, the effect of position on the wear debris detection is not well understood. In this paper, a novel detection system in which the position of wear debris pass through could be adjusted precisely is designed. By changing the position in horizontal or vertical direction, the inductive signals of the wear debris were acquired. In the horizontal direction, the experimental results show that the amplitude of the inductive signal first increases and then decreases when the position changes from the center of the planar inductor to the outer. The maximum inductive signal appears when the wear debris pass through the edge of the inner coil, which is 20% higher than that for the center and much higher than that for the edge of outer coil. In the vertical direction, the signal decreases almost linearly when the position is away from the planar inductor. For every 0.1 mm step far away the planar inductor, the signal amplitude drops by approximately 10%. The variation trend of our experimental results is consistent with the numerical simulation results of magnetic intensity around the planar inductor.

Improving Sensitivity of a Micro Inductive Sensor for Wear Debris Detection with Magnetic Powder Surrounded

The inductive detection of wear debris in lubrication oil is an effective method to monitor the machine status. As the wear debris is usually micro scale, a micro inductive sensor is always used to detect them in research papers or high-tech products. However, the improvement of detection sensitivity for micro inductive sensors is still a great challenge, especially for early wear debris of 20 μm or smaller diameter. This paper proposes a novel method to improve the detection sensitivity of a micro inductive sensor. Regarding the magnetic powder surrounding the sensor, the magnetic field in the core of the sensor where the wear debris pass through would be enhanced due to the increased relative permeability. Thus, the inductive signal would be improved and the detection sensitivity would be increased. It is found that the inductive signal would linearly increase with increasing the concentration of the magnetic powder and this enhancement would also be effective for wear debris of different sizes. In addition, the detection limit of the micro inductive sensor used in our experiment could be extended to 11 μm wear debris by the proposed method.

Annelids shed light on the evolution of spiralian development

Spiralian development is characterized by stereotypic cell geometry and spindle orientation in early cleavage stage embryos, as well as conservation of ultimate fates of descendent clones. Diverse taxa such as molluscs, annelids, flatworms, and nemerteans exhibit spiralian development, but it is a mystery how such a conserved developmental program gives rise to such diverse body plans. This review highlights examples of variation during early development among spiralians, emphasizing recent experimental studies in the annelid Capitella teleta Blake, Grassle and Eckelbarger, 2009. Intracellular fate mapping studies in C. teleta reveal that many of its cells’ fates are shared among spiralians, but it also has a novel origin for trunk mesoderm (3c and 3d micromeres). Studies have identified an inductive signal in spiralians that has “organizing activity” and that influences cell fates in the surrounding embryo. Capitella teleta also has an organizing activity; however, surprisingly, it is localized to a different cell, it signals at a different developmental stage, and likely utilizes a distinct molecular signaling pathway compared with that in molluscs. A model is presented to provide a mechanistic explanation of evolutionary changes in the cellular identity of the organizer. Detailed experimental investigations in spiralian embryos demonstrate variation in developmental features that may influence the evolution of novel forms.