Surface-enhanced Raman scattering (SERS) tags have proven to be excellent labels for tissue bioimaging because of their low interference from biological matrices, high photostability, and possibility for using as theranostic agents. Although SERS tags are widely used for the imaging of tumors in vivo, in practice, the low contrast of the tag accumulation in the tissue and strong light scattering can significantly affect their detectability. In this work, we studied these effects by using a phantom of tumor tissue with incorporated SERS tags. The phantom is a 2 mm sphere of calcium alginate with incorporated SERS tags at a concentration of 0.625 × 108–2 × 109 cm−3. To simulate the surrounding medium with differing turbidities, the phantom was placed in a 4 mm thick agarose gel containing intralipid at a concentration of 0–1%. SERS bioimaging was carried out using standard backscattering geometry with different light focusing conditions. We found that shielding the phantom with a turbid medium led not only to a decrease in detectability but also to a decrease in the apparent size of the imaging object. Our results can help develop more accurate algorithms for processing SERS data for bioimaging.
Simulation study for cross-sectional absorption distribution in turbid medium using spatially resolved backscattered light with lateral scanning and one-dimensional solution of nonlinear inverse problem
