scholarly journals Tumor Phantom with Incorporated SERS Tags: Detectability in a Turbid Medium

Photonics ◽  
2021 ◽  
Vol 8 (5) ◽  
pp. 144
Author(s):  
Boris Khlebtsov ◽  
Daniil Bratashov ◽  
Andrey Burov ◽  
Nikolai Khlebtsov
Keyword(s):  
Surrounding Medium ◽  
Apparent Size ◽  
Turbid Medium ◽  
Strong Light ◽  
Low Contrast ◽  
Surface Enhanced Raman ◽  
Enhanced Raman Scattering ◽  
Theranostic Agents ◽  
Sers Tags

Surface-enhanced Raman scattering (SERS) tags have proven to be excellent labels for tissue bioimaging because of their low interference from biological matrices, high photostability, and possibility for using as theranostic agents. Although SERS tags are widely used for the imaging of tumors in vivo, in practice, the low contrast of the tag accumulation in the tissue and strong light scattering can significantly affect their detectability. In this work, we studied these effects by using a phantom of tumor tissue with incorporated SERS tags. The phantom is a 2 mm sphere of calcium alginate with incorporated SERS tags at a concentration of 0.625 × 108–2 × 109 cm−3. To simulate the surrounding medium with differing turbidities, the phantom was placed in a 4 mm thick agarose gel containing intralipid at a concentration of 0–1%. SERS bioimaging was carried out using standard backscattering geometry with different light focusing conditions. We found that shielding the phantom with a turbid medium led not only to a decrease in detectability but also to a decrease in the apparent size of the imaging object. Our results can help develop more accurate algorithms for processing SERS data for bioimaging.

scholarly journals Optical Diagnostic Based on Functionalized Gold Nanoparticles

2019 ◽  
Vol 20 (18) ◽  
pp. 4346 ◽  
Author(s):  
Jiemei Ou ◽  
Zidan Zhou ◽  
Zhong Chen ◽  
Huijun Tan
Keyword(s):  
Optical Properties ◽  
Future Perspective ◽  
Au Nps ◽  
Optical Diagnostic ◽  
Surface Enhanced ◽  
Surface Enhanced Raman ◽  
Enhanced Raman Scattering ◽  
Diagnostic Applications

Au nanoparticles (NPs) possess unique physicochemical and optical properties, showing great potential in biomedical applications. Diagnostic spectroscopy utilizing varied Au NPs has become a precision tool of in vitro and in vivo diagnostic for cancer and other specific diseases. In this review, we tried to comprehensively introduce the remarkable optical properties of Au NPs, including localized surfaces plasmon resonance (LSPR), surface-enhanced Raman scattering (SERS), and metal-enhanced fluorescence (MEF). Then, we highlighted the excellent works using Au NPs for optical diagnostic applications. Ultimately, the challenges and future perspective of using Au NPs for optical diagnostic were discussed.

scholarly journals Common Aspects Influencing the Translocation of SERS to Biomedicine

2018 ◽  
Vol 25 (35) ◽  
pp. 4638-4652 ◽  
Author(s):  
Dionysia Tsoutsi ◽  
Marcos Sanles-Sobrido ◽  
Andreu Cabot ◽  
Pilar-Rivera Gil
Keyword(s):  
Raman Scattering ◽  
State Of The Art ◽  
Surface Enhanced Raman Scattering ◽  
Surface Enhanced ◽  
Surface Enhanced Raman ◽  
Enhanced Raman Scattering ◽  
Diagnostic Technologies ◽  
The Impact

This review overviews the impact in biomedicine of surface enhanced. Raman scattering motivated by the great potential we believe this technique has. We present the advantages and limitations of this technique relevant to bioanalysis in vitro and in vivo and how this technique goes beyond the state of the art of traditional analytical, labelling and healthcare diagnostic technologies.

scholarly journals Correlation of surface-enhanced Raman scattering (SERS) with the surface density of gold nanoparticles: evaluation of the critical number of SERS tags for a detectable signal

2019 ◽  
Vol 10 ◽  
pp. 1016-1023 ◽  
Author(s):  
Vincenzo Amendola
Keyword(s):  
Gold Nanoparticles ◽  
Raman Scattering ◽  
Surface Density ◽  
Surface Enhanced Raman Scattering ◽  
Operating Conditions ◽  
Acquisition Time ◽  
Surface Enhanced ◽  
Surface Enhanced Raman ◽  
Enhanced Raman Scattering ◽  
Sers Tags

The use of plasmonic nanotags based on the surface-enhanced Raman scattering (SERS) effect is highly promising for several applications in analytical chemistry, biotechnological assays and nanomedicine. To this end, a crucial parameter is the minimum number of SERS tags that allows for the collection of intense Raman signals under real operating conditions. Here, SERS Au nanotags (AuNTs) based on clustered gold nanoparticles are deposited on a substrate and analyzed in the same region using Raman spectroscopy and transmission electron microscopy. In this way, the Raman spectra and the surface density of the SERS tags are correlated directly, showing that 1 tag/µm2 is enough to generate an intense signal above the noise level at 633 nm with an excitation power of only 0.65 mW and an acquisition time of just 1 s with a 50× objective. The AuNT density can be even lower than 1 tag/µm2 when the acquisition time is extended to 10 s, but must be increased to 3 tags/µm2 when a 20× objective is employed under the same excitation conditions. In addition, in order to observe a linear response, it was found that 10 SERS AuNTs inside the probed area are required. These findings indicate that a better signal-to-noise ratio requires high-magnification optics, while linearity versus tag number can be improved by using low-magnification optics or a high tag density. In general the suitability of plasmonic SERS labels for ultrasensitive analytical and biomedical applications is evident.

Carboxy-terminated immuno-SERS tags overcome non-specific aggregation for the robust detection and localization of organic media in artworks

The Analyst ◽  
2015 ◽  
Vol 140 (17) ◽  
pp. 5971-5980 ◽  
Author(s):  
E. A. Perets ◽  
A. S. D. S. Indrasekara ◽  
A. Kurmis ◽  
N. Atlasevich ◽  
L. Fabris ◽  
...  
Keyword(s):  
Surface Coating ◽  
Organic Media ◽  
Binding Media ◽  
Robust Detection ◽  
Surface Enhanced ◽  
Surface Enhanced Raman ◽  
Enhanced Raman Scattering ◽  
Detection And Localization ◽  
Proteinaceous Binding Media ◽  
Sers Tags

Surface-enhanced Raman scattering (SERS) nano-tags with a carboxy-terminated PEG surface coating overcome non-specific aggregation when applied for the immunological detection and localization of proteinaceous binding media in art samples.

scholarly journals Binary Surfactant–Mediated Tunable Nanotip Growth on Gold Nanoparticles and Applications in Photothermal Catalysis

2021 ◽  
Vol 9 ◽  
Author(s):  
Xiaohu Mi ◽  
Tingting Zhang ◽  
Baobao Zhang ◽  
Min Ji ◽  
Bowen Kang ◽  
...  
Keyword(s):  
Raman Scattering ◽  
Surface Enhanced Raman Scattering ◽  
Au Nanoparticle ◽  
Plasmonic Nanostructures ◽  
Hexadecyltrimethylammonium Bromide ◽  
Strong Light ◽  
Wide Range ◽  
Surface Enhanced ◽  
Surface Enhanced Raman ◽  
Enhanced Raman Scattering

Plasmonic nanostructures with sharp tips are widely used for optical signal enhancement because of their strong light-confining abilities. These structures have a wide range of potential applications, for example, in sensing, bioimaging, and surface-enhanced Raman scattering. Au nanoparticles, which are important plasmonic materials with high photothermal conversion efficiencies in the visible to near-infrared region, have contributed greatly to the development of photothermal catalysis. However, the existing methods for synthesizing nanostructures with tips need the assistance of poly(vinylpyrrolidone), thiols, or biomolecules. This greatly hinders signal detection because of stubborn residues. Here, we propose an efficient binary surfactant–mediated method for controlling nanotip growth on Au nanoparticle surfaces. This avoids the effects of surfactants and can be used with other Au nanostructures. The Au architecture tip growth process can be controlled well by adjusting the ratio of hexadecyltrimethylammonium bromide to hexadecyltrimethylammonium chloride. This is due to the different levels of attraction between Br−/Cl− and Au3+ ions. The surface-enhanced Raman scattering and catalytic abilities of the synthesized nanoparticles with tips were evaluated by electromagnetic simulation and photothermal catalysis experiments (with 4-nitrothiophenol). The results show good potential for use in surface-enhanced Raman scattering applications. This method provides a new strategy for designing plasmonic photothermal nanostructures for chemical and biological applications.

scholarly journals Bioanalytical applications of surface-enhanced Raman spectroscopy: de novo molecular identification

2017 ◽  
Vol 36 (4) ◽  
Author(s):  
Anh H. Nguyen ◽  
Emily A. Peters ◽  
Zachary D. Schultz
Keyword(s):  
De Novo ◽  
Surface Enhanced Raman Spectroscopy ◽  
Clinical Diagnostics ◽  
Active Materials ◽  
Intrinsic Signal ◽  
Surface Enhanced ◽  
Surface Enhanced Raman ◽  
Enhanced Raman Scattering ◽  
Bioanalytical Applications ◽  
Sers Tags

AbstractSurface-enhanced Raman scattering (SERS) has become a powerful technique for trace analysis of biomolecules. The use of SERS-tags has evolved into clinical diagnostics; the enhancement of the intrinsic signal of biomolecules on SERS active materials shows tremendous promise for the analysis of biomolecules and potential biomedical assays. The detection of the

scholarly journals Silica-coated dimers of silver nanospheres as surface-enhanced Raman scattering tags for imaging cancer cells

2013 ◽  
Vol 3 (3) ◽  
pp. 20120092 ◽  
Author(s):  
Xiaohu Xia ◽  
Weiyang Li ◽  
Yu Zhang ◽  
Younan Xia
Keyword(s):  
Raman Scattering ◽  
Cancer Cells ◽  
Hot Spot ◽  
High Sensitivity ◽  
Silica Coating ◽  
Surface Enhanced Raman Scattering ◽  
Surface Enhanced ◽  
Surface Enhanced Raman ◽  
Enhanced Raman Scattering ◽  
Sers Tags

Surface-enhanced Raman scattering (SERS) tags have been actively explored as a multiplexing platform for sensitive detection of biomolecules. Here, we report a new type of SERS tags that was fabricated by sequentially functionalizing dimers made of 50 nm Ag nanospheres with 4-mercaptobenzoic acid as the Raman reporter molecule, silica coating as a protective shell and antibody as a targeting ligand. These dimer-based tags give highly enhanced and reproducible Raman signals owing to the presence of a well-defined SERS hot spot at the junction between two Ag nanospheres in the dimer. The SERS enhancement factor (EF) of an individual dimer tag supported on a glass slide can reach a level as high as 4.3 × 10 6 . In comparison, the EFs dropped to 2.8 × 10 5 and 8.7 × 10 5 , respectively, when Ag nanospheres and nanocubes with sizes similar to the spheres in the dimer were used to fabricate the tags using similar procedures. The SERS signals from aqueous suspensions of the dimer-based tags also showed high intensity and good stability. Potential use of the dimer-based tags was demonstrated by imaging cancer cells overexpressing HER2 receptors with good specificity and high sensitivity.

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