Gut Microbiota of Pigs Metabolizes Extracts of Filipendula ulmaria and Orthosiphon aristatus–Herbal Remedies Used in Urinary Tract Disorders

Urinary tract infections influence the mortality rate in pigs and are linked to extensive antibiotic usage in the farm industry. Filipendula ulmaria (L.) Maxim. and Orthosiphon aristatus (Blume) Miq. are widespread medicinal plants traditionally used to treat urinary tract disorders. As their preparations are orally administered, the metabolism of their constituents by gut microbiota before absorption should be considered. Until now, no experiments had been performed to describe the biotransformation of tthose plantsʼ extracts by animal gut microbiota. The study evaluates the influence of pig intestinal microbiota on the structure of active compounds in flowers of F. ulmaria and leaves of O. aristatus. The incubations of the extracts with piglet gut microbiota were performed in anaerobic conditions, and the samples of the batch culture were collected for 24 h. In F. ulmaria, the main metabolites were quercetin and kaempferol, which were products of the deglycosylation of flavonoids. After 24 h incubation of O. aristatus extract with the piglet gut microbiota, 2 main metabolites were observed. One, tentatively identified as 3-(3-dihydroxyphenyl)propionic acid, is likely the primary metabolite of the most abundant depsides and phenolic acids. The results confirm the formation of the compounds with anti-inflammatory and diuretic activity in the microbiota cultures, which might suggest F. ulmaria and O. aristatus for treating urinary tract disorders in piglets. Based on the similarities of human and pig gut microbiota, the pig model can help estimate the metabolic pathways of natural products in humans.

Barriers affecting patient adherence to intermittent self-catheterisation

Intermittent self-catheterisation (ISC) is now considered the standard of care for most patients with neurological conditions and associated lower urinary tract disorders. Numerous societies, led by the International Continence Society, are in agreement on the effectiveness of ISC. Use of intermittent catheters is reported to reduce the risk of catheter-associated urinary tract infection compared with indwelling urinary catheters while improving patient comfort and quality of life. However, previous studies have shown that, despite the benefits of ISC, it will not guarantee behavior change and the integration of this procedure into the daily life of patients. Patients may encounter internal (related to the patient themselves) and external (related to their environment) difficulties. Identifying these obstacles early will help promoting ISC success. This review aims to identify internal and external barriers related to ISC and to propose adequate solutions to avoid them.

Clinical, ultrasonographic, and postmortem findings in sheep and goats with urinary tract disorders

Background and Aim: In sheep and goats, most urinary disorders are incidental findings at the postmortem examination and do not present clinically. As such, the diagnosis of renal diseases in sheep and goats can be overlooked. Therefore, this study was carried out on sheep and goats to describe the clinical, ultrasonography (USG), and postmortem findings of various disorders affecting the urinary tract. Materials and Methods: A total of 58 animals (30 sheep and 28 goats) were examined, as well as were ten healthy control animals (five sheep and five goats) for a comparison. The animals were referred for various causes, including anorexia, frequent and painful urination, hematuria, decreased body weight, oliguria, and anuria. Results: The sheep and goats were categorized into ten groups of symptoms and disorders: Pelvic abscessation, pigmented urine, renal failure, pyelonephritis, cystitis, hydronephrosis, rupture of the urethra, rupture of the urinary bladder, rupture of the urinary bladder together with the urethra, and paralysis of the urinary bladder. Clinical presentation, USG, and postmortem examination results for each group were detailed. Many clinical presentations were nonspecific. USG examination of the urinary tract significantly facilitated the verification of the previously mentioned disorders. Conclusion: USG demonstrated superior performance in the early and confirmatory diagnosis of urinary disorders in sheep and goats. Therefore, it is recommended that routine USG be the preferred imaging modality for examining sheep and goats with urinary disorders and for determining their prognosis.

Obsessive-Compulsive Disorders and Functional Urinary Disorders: A Fortuitous Association?

Although psychological factors are known to affect bladder and bowel control, the occurrence of functional urinary disorders in patients with psychiatric disorders has not been well-studied or described. A higher prevalence of functional lower urinary tract disorders have also been reported amongst patients with obsessive-compulsive (OC) disorders. A systematic literature search of PubMed, EMBASE, OVID Medline, PsycINFO, Clinical Trials Register of the Cochrane Collaboration Depression, Anxiety and Neurosis Group (CCDANTR), Clinicaltrials.gov and Google Scholar databases found five observational studies on the topic. Unfortunately, as only one study had a (healthy) control group, a meta-analytic approach was not possible. Overall, patients with OC symptoms appeared to have increased occurrence of functional urinary symptoms, e.g., overactive bladder, increase in urgency, frequency, incontinence and enuresis. This was even more common amongst patients with Pediatric Autoimmune Neuropsychiatric Disorder Associated with Streptococcal Infections (PANDAS) or Pediatric Acute-onset Neuropsychiatric Syndrome (PANS) as opposed to patients with OCD alone. Several biological and behavioural mechanisms and treatment approaches were discussed. However, as the current evidence base was significantly limited and had moderate to serious risk of bias, no strong inferences could be drawn. Further well-designed cohort studies are necessary to better elucidate the observed associations and their management.

Chemical Composition, Diuretic, and Antityrosinase Activity of Traditionally Used Romanian Cerasorum stipites

Cherry stems (CS) represent a by-product intensively used in Eastern European countries as a traditional remedy for urinary tract disorders. Ethnopharmacological evidences sustain the use of CS as aqueous preparations (infusion and decoction), but few data were previously reported about phytochemical profile and pharmacological potential of CS hydroalcoholic extracts. In this regard, we aimed to evaluate the phenolic profile, in vitro antioxidant and tyrosinase inhibitory potential, and in vivo diuretic activity of 70% hydroethanolic cherry stems extract and cherry stems decoction (CSD). LC-DAD-ESI/MSn analysis revealed the presence of flavonoid-type compounds as main constituents for both preparations, especially flavanones (naringenin glycosides). Antioxidant activity evaluated through DPPH, ABTS, and FRAP methods was superior for cherry stems extract, probably due to the presence of phenolic-derived compounds in higher amounts than CSD. On the other hand, tyrosinase inhibitory potential and diuretic effect exerted by CSD were stronger, highlighting that other types of hydrophilic secondary metabolites are responsible for this bioactivity. Overall, our findings indicate that CS preparations could be used as promising mild diuretic agents and encourage further investigations regarding the correlation between their chemical composition and bioactive potential.

Ethnobotanical Survey of Medicinal Herbs in the Western Region in Syria (Latakia and Tartus)

ContextThe use of traditional Arabic medicine (TAM) has spread to treat various diseases in Syria since ancient time. They are cost-effective with fewer side effects and are more suitable for long-term use compared with chemically synthesized medicines.ObjectiveWe conducted ethnobotanical and ethno-medicine research on plants traditionally used to treat various diseases in Western region of Syria. MethodsField surveys were conducted during July 2019-December 2020 to document ethnobotanical information through oral interviews and designed semi-structured questionnaire from local herbalists (Tabib Arabi) and the elderly people and others who were familiar with traditional uses of plants particularly for medicinal. The queries were repeatedly made to increase the reliability of the data, during the field survey of 475 local inhabitants of 70 villages. ResultsIn this research we listed 258 medicinal plants belonging to 83 families in alphabetical order with the parts used and the method of preparation according to their therapeutic use. Of these studied plants, 185 are used to treat digestive disorders, 118 for respiratory diseases, 91 for several skin diseases, 87 for kidney and urinary tract disorders, 78 for diabetes, 18 for nervous system disorders, and a few plants for treatment the other diseases which are commonly spread in study area. The calculated results of medicinal use-value (MUV) showed that Olea europaea L., Origanum syriacum L. was ranked first (MUV= 0.934), while the lowest value was found for Fumana thymifolia (L.) Spach and Cistus creticus L. (MUV= 0.003).ConclusionA large portion of the uses of medicinal plants mentioned in the research are still under study. There is no doubt that its study will provide many new data that could contribute to further pharmacological discoveries by identifying the active ingredients and their mechanism of effect by doing a lot of pharmacological work to confirm the alleged biological activities of these plants.