scholarly journals Application of Autochthonous Yeast Saccharomyces cerevisiae XG3 in Treixadura Wines from D.O. Ribeiro (NW Spain): Effect on Wine Aroma

Fermentation ◽  
2021 ◽  
Vol 7 (1) ◽  
pp. 31
Author(s):  
Pilar Blanco ◽  
María Vázquez-Alén ◽  
Teresa Garde-Cerdán ◽  
Mar Vilanova
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Pilot Scale ◽  
Sensory Level ◽  
Wine Aroma ◽  
Nw Spain ◽  
Volatile Phenols ◽  
Yeast Saccharomyces Cerevisiae ◽  
Spontaneous Fermentation ◽  
Autochthonous Yeast ◽  
Active Dry Yeast

Yeast plays an essential role in winemaking. Saccharomyces cerevisiae strains involved in fermentation determine the chemical and sensory characteristics of wines. S. cerevisiae XG3, isolated in Galicia (NW Spain), has desirable oenological potential, which has been proved at a pilot scale to produce quality wines. This study applies XG3 as active dry yeast at an industrial scale for Treixadura wine elaboration, and compares it with commercial yeast and spontaneous fermentation within three wineries included in Denomination of Origin Ribeiro over two vintages. Fermentations are monitored using conventional methods, and microbiological implantation controls are carried out by mtDNA-RFLPs analysis. Wine basic chemical parameters are determined using OIV official methodology, and volatile aroma compounds are determined by GC-MS. Finally, wine sensory analysis is also performed. S. cerevisiae XG3 shows an acceptable implantation ability—as compared to commercial control strains. The wines from XG3 have a higher total acidity and lower alcohol content. Their volatile composition differs from control wines, since XG3 produces significantly higher concentrations of acetates, volatile acids, esters and volatile phenols, depending on the vintage and winery. However, lower differences are perceived at the sensory level, where fruity and floral descriptors are perceived by the panellists in XG3 wines. Therefore, XG3 constitutes an alternative to differentiate Treixadura wines.

DEPENDENCE OF THE CONCENTRATION OF AROMATIC COMPONENTS AND ORGANIC ACIDS IN WHITE TABLE WINE MATERIAL ON THE METHOD OF TECHNOLOGICAL PROCESSING

2019 ◽  
Author(s):  
А.А. АЛЕКСЕЕВА ◽  
Н.М. АГЕЕВА ◽  
В.Е. СТРУКОВА ◽  
Ю.Ф. ЯКУБА ◽  
Л.И. СТРИБИЖЕВА
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Organic Acids ◽  
Methyl Acetate ◽  
Soft Mode ◽  
Higher Alcohols ◽  
Yeast Saccharomyces Cerevisiae ◽  
Grape Must ◽  
Aromatic Components ◽  
Technological Processing ◽  
Active Dry Yeast

Рассмотрено влияние способа технологической обработки белого столового виноматериала на состав и концентрацию компонентов ароматобразующего комплекса и органических кислот. В качестве объекта исследований был белый столовый виноматериал Пино Блан урожая 2018 г. Виноматериал получен по следующей схеме: переработка винограда в мягком режиме путем пневмопрессования, отделение сусла первой фракции, осветление сусла с предварительным охлаждением, внесение подкормки для дрожжей АF1 в количестве 0,9 г/дм3, сбраживание сусла активными сухими дрожжами Saccharomyces cerevisiae раса WT1 (Германия). Установлено, что выдержка виноматериала на дрожжевом осадке привела к снижению концентрации винной и янтарной кислот, количество яблочной и лимонной кислот не изменилось. Отмечено увеличение количества молочной, уксусной, валериановой и изовалериановой кислот. В результате обработки бентонитом снизились концентрации валериановой и изовалериановой кислот. В результате брожения виноградного сусла в присутствии сухих дрожжей образуется большое количество эфиров, определяющих специфические оттенки и формирующих аромат виноматериала. Выдержка виноматериала на дрожжевом осадке способствовала повышению концентрации ацетальдегида, метилацетата, ацеталей, высших спиртов, эфира этилвалериата. Количество других эфиров и терпеновых соединений значительно уменьшилось. Обработка виноматериала бентонитом привела к уменьшению количества ароматобразующих компонентов на 8 16. The influence of the method of technological processing of white table wine material on the composition and concentration of components of the aromatic complex and organic acids is considered. White table wine material Pinot Blanc harvest 2018 was as an object of research. Wine material is obtained according to the following scheme: processing of grapes in the soft mode by pneumomassage, the separation of the wort of the first fraction, wort clarification with preliminary cooling to fertilize for yeast AF1 in an amount of 0,9 g/dm3, the fermentation wort active dry yeast Saccharomyces cerevisiae, race, WT1 (Germany). It was found that the aging of wine material on yeast sediment led to a decrease in the concentration of tartaric and succinic acids, the amount of malic and citric acids has not changed. An increase in the amount of lactic, acetic, valerian and isovaleric acids was noted. Concentrations of valerian and isovaleric acids decreased as a result of treatment by bentonite. A large number of esters, which determine the specific shades and form the aroma of wine material, is formed as a result of fermentation of grape must in the presence of dry yeast. The wine material aging on yeast sediment has contributed to increasing concentration in wine acetaldehyde, and methyl acetate, acetals, higher alcohols, ether of ethylmalonate, a number of other esters and terpenic compounds is significantly reduced. Treatment of wine material by bentonite led to a decrease in the number of aromatic components by 8 16.

scholarly journals Selection of Saccharomyces cerevisiae Starter Strain for Merwah Wine

Fermentation ◽  
2020 ◽  
Vol 6 (2) ◽  
pp. 43
Author(s):  
Nadine Feghali ◽  
Angela Bianco ◽  
Giacomo Zara ◽  
Edouard Tabet ◽  
Chantal Ghanem ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Acetic Acid ◽  
Pilot Scale ◽  
Molecular Monitoring ◽  
Technological Parameters ◽  
Sensorial Analysis ◽  
Spontaneous Fermentation ◽  
Wine Production ◽  
Wild Yeasts ◽  
Selection Of

In order to select Saccharomyces cerevisiae starter strains for ‘‘Merwah’’ wine production, three strains (M.6.16, M.10.16, and M.4.17) previously isolated from ‘‘Merwah’’ must and characterized at the lab scale were tested in pilot-scale fermentation in a Lebanese winery during the 2019 vintage. The three inoculated musts were compared to that obtained with a spontaneous fermentation. During the fermentations, must samples were taken to evaluate the dominance of the inoculated strains, and at the end of fermentation, the obtained wines were subjected to chemical and sensorial characterization. Molecular monitoring by interdelta analysis revealed that only M.4.17 was able to complete the fermentation and dominate over the wild yeasts. Based on the analysis of principal technological parameters (i.e., residual sugar, fermentative vigor, sulfur production, and acetic acid) and sensorial analysis of the wines obtained, M.4.17 was selected as an adequate starter for the production of typical ‘‘Merwah’’ wine.

scholarly journals Saccharomyces cerevisiae Strain Diversity Associated with Spontaneous Fermentations in Organic Wineries from Galicia (NW Spain)

Fermentation ◽  
2020 ◽  
Vol 6 (3) ◽  
pp. 89
Author(s):  
David Castrillo ◽  
Noemi Neira ◽  
Pilar Blanco
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Statistical Tests ◽  
Wide Distribution ◽  
Nw Spain ◽  
Wine Quality ◽  
Spontaneous Fermentation ◽  
Strain Diversity ◽  
Spontaneous Fermentations ◽  
Different Strains ◽  
Yeast Isolation

Yeast play an essential role in wine quality. The dynamics of yeast strains during fermentation determine the final chemical and sensory characteristics of wines. This study aims to evaluate the Saccharomyces cerevisiae strains diversity in organic wineries from Galicia (NW Spain). Samples from spontaneous fermentations were taken in five wineries over three consecutive years (2013 to 2015). The samples were transported to the laboratory and processed following standard methodology for yeast isolation. S. cerevisiae strains were differentiated by mDNA-RFLPs. A total of 66 different strains were identified. Some of them presented a wide distribution and appeared in several wineries. However, other strains were typical from a specific winery. Similarity analysis using two different statistical tests showed significant differences in strain diversity among wineries. The results also revealed high biodiversity indexes; however, only some strains showed an important incidence in their distribution and frequency. Our findings confirmed that spontaneous fermentation favored the existence of a high S. cerevisiae strain diversity in organic wineries from Galicia. The presence of different yeasts during fermentation, specially winery-specific strains, contribute to increased wine complexity and differentiation.

Isolation of Saccharomyces cerevisiae YDJ05 from the Spontaneous Fermentation Pear Wine and Study of the Yeast Growth Dynamics during the Association Fermentation

2010 ◽  
Vol 156-157 ◽  
pp. 266-271
Author(s):  
Da Wei Zhang ◽  
Wenbin Dong ◽  
Lei Jin ◽  
Jie Zhang ◽  
Yuan Chang Jin
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Ethanol Concentration ◽  
Ethanol Yield ◽  
Growth Dynamics ◽  
Yeast Growth ◽  
Yeast Saccharomyces Cerevisiae ◽  
Spontaneous Fermentation ◽  
Potential Source ◽  
Yeast Strains ◽  
Better Than

Five preponderant yeast strains (YDJ01, YDJ02, YDJ03, YDJ04 and YDJ05) were isolated from the spontaneous fermentation pear wine as source of yeast for wine making from pear. Ethanol yield of YDJ05 was the highest and its using rapidity of the sugar was the most quickly. YDJ05 was identified as Saccharomyces cerevisiae and named Saccharomyces cerevisiae YDJ05. In addition, the fermentation dynamics of three yeast strains (Saccharomyces cerevisiae YDJ05, “Angle” yeast and Saccharomyces cerevisiae GIM2.39) were studied including single fermentation and associated fermentation. The fermentative behavior of three strains changed in association fermentations (Saccharomyces cerevisiae YDJ05 and “Angle” yeast, Saccharomyces cerevisiae YDJ05 and Saccharomyces cerevisiae GIM2.39). Results indicated that the qualities of pear wines made from association fermentations were better than that of single fermentations. The pear wine fermented associated by Saccharomyces cerevisiae YDJ05 and Saccharomyces cerevisiae GIM2.39 was the best in quality by sensory evaluation among all pear wines whose ethanol concentration was 10.3% (v/v). Saccharomyces cerevisiae YDJ05 and mai could be excellent potential source of strains.

scholarly journals Selected Indigenous Saccharomyces cerevisiae Strains as Profitable Strategy to Preserve Typical Traits of Primitivo Wine

Fermentation ◽  
2019 ◽  
Vol 5 (4) ◽  
pp. 87 ◽  
Author(s):  
Capece ◽  
Pietrafesa ◽  
Siesto ◽  
Romaniello ◽  
Condelli ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Pilot Scale ◽  
Sensory Characteristics ◽  
Spontaneous Fermentation ◽  
Wine Production ◽  
Grape Must ◽  
High Dominance ◽  
Very High ◽  
Grape Must Fermentation

Wine production by inoculated fermentation with commercial Saccharomyces cerevisiae strains is an ordinary practice in modern winemaking in order to assure the final quality of wine, although this procedure results in the production of highly homogeneous wines. The use of indigenous selected starters represents a useful tool to control alcoholic grape must fermentation, safeguarding the typical sensory characteristics of wine produced from specific regions. In this study, we selected three indigenous S. cerevisiae strains among 16 indigenous strains previously isolated from the spontaneous fermentation of Primitivo grapes, which were collected from the vineyards of three different cellars. The three selected starters (one for each cellar) were tested during fermentations at pilot scale by performing in each cellar two trials: one with an indigenous starter (specific for the winery), and one with the commercial starter AWRI796 (common to all the cellars). Starter dominance ability and influence on aromatic quality of the wine were used as criteria to test the suitability of these indigenous starters to be used at the cellar scale. The results obtained in this study showed that the indigenous strains were characterized by very high dominance ability, and the aromatic quality of wine was strongly influenced both by the inoculated strain and the interaction strain/grape must.

scholarly journals The effects of feeding benzoic acid and/or live active yeast (Saccharomyces cerevisiae) on beef cattle performance, feeding behaviour, and carcass characteristics

2021 ◽  
Author(s):  
M S Williams ◽  
I B Mandell ◽  
B M Bohrer ◽  
K M Wood
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Benzoic Acid ◽  
Feeding Behaviour ◽  
Carcass Characteristics ◽  
Animal Performance ◽  
Eating Rate ◽  
Yeast Saccharomyces Cerevisiae ◽  
Treatment Groups ◽  
Ruminal Ph ◽  
Active Dry Yeast

Abstract Fifty-nine Angus-cross finishing steers were used to evaluate benzoic acid, active dry yeast (Saccharomyces cerevisiae), or a combination of benzoic acid and active dry yeast when supplemented in a high-grain finishing diet on live animal performance, feeding behaviour, and carcass characteristics. Steers were fed a high-grain diet for the final 106 d of finishing. Treatments were; no additional supplementation (CON), 0.5% benzoic acid (ACD), 3 grams per head per day active dry S. cerevisiae (YST), or both 0.5% benzoic acid and 3 grams/head per day S. cerevisiae (AY). Steers were weighed every 14 d, and ultrasound was performed for rib and rump fat thickness at the beginning (d 1), middle (d 57), and end (d 99) of the experiment. Insert feeding stations were used to collect individual feeding behaviour data and DMI daily throughout. Blood samples were collected on d 21-22 and d 99-101 to assess plane of nutrition and metabolism. Ruminal fluid samples were collected by oral gavage 4 weeks prior to slaughter. Carcass characteristics were examined at a federally inspected slaughter facility. Data were analyzed using PROC GLIMMIX of SAS with initial body weight (BW) as a covariate. Benzoic acid supplementation increased (P = 0.002) overall dry matter intake (DMI) compared to YST and CON steers, which may be due to a faster eating rate (P ≤ 0.008). Animal performance parameters (BW, average daily gain, feed conversion, and ultrasound fat depth) were not different (P ≥ 0.11) among treatment groups. Aspartate aminotransferase concentration was greatest (P ≤ 0.01) for YST steers, which may have been reflected in numerically greater liver abscesses. Carcass traits did not differ (P ≥ 0.33) among treatment groups. Ruminal pH was greater (P = 0.006) for ACD steers than AY steers (pH of 6.16 vs. 5.66, respectively), which indicated that there may be an interactive effect between benzoic acid and active dry yeast. To summarize, steers fed a high-grain finishing diet supplemented with benzoic acid, active dry yeast, or both benzoic acid and active dry yeast had similar growth performance and carcass characteristics compared to those without supplementation. However, the addition of benzoic acid alone increased DMI, variation in DMI, eating rate, and ruminal pH. Future studies are warranted to further investigate the impacts of benzoic acid on the ruminal environment of feedlot cattle

288 Inclusion Effects of Quebracho (Schinopsis Balansaie) Extract and Active Dry Yeast (Saccharomyces Cerevisiae) in Beef Cattle Limit-fed a Grower Ration

2021 ◽  
Vol 99 (Supplement_3) ◽  
pp. 157-157
Author(s):  
Madeline E Rivera ◽  
Luiz Fernando Dias Batista ◽  
Aaron B Norris ◽  
Genevieve M D’Souza ◽  
Luis O Tedeschi
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Condensed Tannin ◽  
Plant Secondary Metabolites ◽  
Gas Production ◽  
Yeast Saccharomyces Cerevisiae ◽  
Combined Supplementation ◽  
Active Dry Yeast ◽  
The Impact ◽  
Grower Diet

Abstract Plant secondary metabolites (PSM) and yeast supplemented in growing cattle have been reported as improving dry matter digestibility (DMD) and reducing enteric methane emissions. This study aimed to evaluate the effect of combined supplementation with condensed tannin (CT; Schinopsis balansae) extract and active dry yeast (ADY; Saccharomyces cerevisiae) on fermentation dynamics, utilizing in vitro gas production (IVGP) technique. A 2 × 2 Latin square design was used to study fermentation patterns of four dietary treatments (CON- no CT and no ADY, ADY alone, CT alone or combined CT + ADY). Animals received daily CT at 1% DM and 10 g of ADY, respectively. On d 0, 7, 14, 21, 28, and 35 rumen inoculum was collected from 23 fistulated steers (284.3 ± 4.1 kg) four hours post-feeding. Samples were incubated under anaerobic conditions at 39oC for 48 h with 200 mg of a grower diet (14.8% CP, 40.6% NDF, 88.5% DM). Gas parameters were analyzed using a mixed linear statistical model. There was a day effect for total gas production (TGP; P < 0.001), non-fiber carbohydrate degradation (P = 0.031) and fractional degradation assuming an asymptote model (P = 0.015). Both asymptote and non-fiber fractional degradation rate estimates had an interaction between Day × TRT (P = 0.001 and 0.0104, respectively). Data were analyzed using polynomial contrasts showed a difference in non-fiber fractional rate of fermentation for CON × CT and CT × ADY (P = 0.052 and 0.054, respectively). This was also true if an asymptote model was assumed (P = 0.0 34 and 0.034, respectively). We concluded that combined supplementation of CT and ADY exhibited similar IVGP trends over time, this may be because animals only received a grower diet at 1.5% shrunk BW. Future studies should investigate the impact of combined supplementation on varying levels of concentrate diets.

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