Regional Variation of Gap Junctional Connections in the Mammalian Inner Retina

The retinas of many species show regional specialisations that are evident in the differences in the processing of visual input from different parts of the visual field. Regional specialisation is thought to reflect an adaptation to the natural visual environment, optical constraints, and lifestyle of the species. Yet, little is known about regional differences in synaptic circuitry. Here, we were interested in the topographical distribution of connexin-36 (Cx36), the major constituent of electrical synapses in the retina. We compared the retinas of mice, rats, and cats to include species with different patterns of regional specialisations in the analysis. First, we used the density of Prox1-immunoreactive amacrine cells as a marker of any regional specialisation, with higher cell density signifying more central regions. Double-labelling experiments showed that Prox1 is expressed in AII amacrine cells in all three species. Interestingly, large Cx36 plaques were attached to about 8–10% of Prox1-positive amacrine cell somata, suggesting the strong electrical coupling of pairs or small clusters of cell bodies. When analysing the regional changes in the volumetric density of Cx36-immunoreactive plaques, we found a tight correlation with the density of Prox1-expressing amacrine cells in the ON, but not in the OFF sublamina in all three species. The results suggest that the relative contribution of electrical synapses to the ON- and OFF-pathways of the retina changes with retinal location, which may contribute to functional ON/OFF asymmetries across the visual field.

Estimating the short-term economic impact of COVID-19 pandemic: A regional perspective

Given the economic crisis related to the current COVID-19 pandemic, decision makers need to better understand how to effectively allocate their limited resources to support the most severely affected regions. In this context our research seeks to identify the regions that are economically vulnerable, as well as those that are more resilient, using information on the industries negatively impacted by travel restrictions and social distancing measures. With this aim in view, we propose a vulnerability index calculated using regions’ location coefficients by activity and the forecasted decline of these activities at national level and test it for the Romanian economy. We argue that regional specialisation itself is not the source of the current problems, but the high dependence of many regional economies on economic activities directly affected by measures designed to mitigate the epidemic impact is the root cause of future economic decline.

FOREIGN OWNERSHIP AS A NEGLECTED FACTOR IN REGIONS’ SMART SPECIALISATION EXPORTS

A region can internationalise in various ways. The question is what type of internationalisation at regional level can be regarded as “smart” and what role should be played in it by foreign owned entities (FOEs)? The paper aims at identifying the role played by FOEs in NUTS-2 smart specialisations’ (SS’s) internationalisation through exports. With the use of a set of PCSE regressions, the paper depicts the role of FOEs in creating SS-compliant exports against the background of their influence on total regional exports. The obtained results proved the positive role of FOEs in regional exports per se and SS-compliant exports. Yet, FOEs differed substantially, as compared to indigenous entities, in terms of product/regional specialisation and technological advancement. The findings imply the importance of FOEs’ embeddedness in regions’ economies and possibility of their incorporation in creating or enhancing regional comparative advantages. The paper contributes by: (i) providing empirical verification of one of the aspects of SS strategy which is exports and establishing comprehensive insight into the evaluation of SS, (ii) depicting the role of FOEs in generating SS-compliant exports, (iii) proposing a set of measures to be used in the extended empirical evaluation of SS consequences in the sphere of exports.

CONNECTING THE NORTHERN ANDES AND THE ATLANTIC. THE ROLE OF INLAND PORTS IN NEW GRANADA'S INTERREGIONAL TRADE (1770-1809)

ABSTRACT This paper analyses the role of Mompox in New Granada's interregional trade during the late colonial period. It focuses on the value, structure and destination of exports of domestic goods from Mompox to markets on the Atlantic and the Andes. By unearthing unexplored sources, this paper provides evidence that will help to understand, indirectly, some issues such as the nature and timing of economic growth, the degree of regional specialisation and, above all, the role of inland ports in the economic geography of the viceroyalty. The paper contends, first, that the region experienced a boom–bust cycle during the late colonial period. The export of domestic goods doubled between 1770 and 1800 but subsequently collapsed during the 1802-1809 years. Second, evidence suggests that the region experienced a process of market deepening and widening. Trade flows, then, played a larger role in shaping the economic history of the region than previously thought.

P100 CD161 levels are reduced in all subpopulations of T-cell colonic mucosal lymphocytes in inflammatory bowel disease

Background CD161 is a type C lectin expressed in NKs cells and peripheral T cells (TCRγδ and αβ, NKTs), enriched in intestinal populations. Its expression can be modulated by infections and inflammation. MAIT cells are a subset of innate antimicrobial T-cells abundant in the mucosa but their role in immunological regulation is still unknown. Aim To measure CD161 levels in subtypes of T-lymphocytes of intestinal mucosa: CD4+, CD8+, double positive (DP,CD4+CD8+), double negative (DN,CD4−CD8−), MAIT cells (CD161+TCRVα7.2+) and intraepithelial cells (CD103+) Methods Twenty-six patients with active inflammatory bowel disease (IBD) without immunosuppressive treatment (n = 9 Crohn’s disease -CD- colon, 9 CD ileum, n = 8 ulcerative colitis -UC- and 10 healthy controls (paired biopsies of ileum, right and left colon) were included. Lymphocyte subpopulations were analysed with LSRFortessa cytometer. Non-parametric Kruskal–Wallis test was applied. Results are expressed as % of median (25–75%IQI). Results In healthy mucosa, we did not find differences related to location in any of CD161 subpopulations except for increase of CD3+CD161+CD103+ and decrease of CD3+CD161+CD103− in left colon compared with right colon and ileum. Regarding MAIT cells, a progressive decrease was observed in distal parts of intestine for CD3+MAIT+CD103+ while CD3+MAIT+CD103− subpopulation has a specular behaviour; CD3+CD8+MAIT+ was increased in ileum compared with colon (Table 1). Conclusion There is a regional specialisation for the subset CD103+ of both CD161+CD103+ and MAIT_CD103+ cells in healthy intestine. CD3+CD161+ T cells are reduced in IBD colonic inflammation and could serve as a marker of active IBD but not to sort between CD and UC.

Measuring the Industrial Concentration and Regional Specialisation of Major Indian Industrial States

This article analyses the influence of industrial disparity and centralisation (concentration) on major industrial states in India. Accordingly, we are using two mathematical models: (a) localisation coefficients to identify major industries that are suffering by industrial concentration and (b) location quotient to measure regional industrial specialisation. Consequently, it shows the natural tendency of distribution of industries in a particular way between the six major Indian industrial states. Therefore, we determine the reasons for lack of industrialisation within those states. Afterwards, this research helps to understand that those regions are either little specialised regions or concentrated with few industries. Especially, policy makers can easily equally distribute through location quotient and localisation coefficient, all manufacturing industries in all six regions. Moreover, this research can reduce the tendency of lack of industrialisation.