Super-resolution imaging of highly curved membrane structures in giant vesicles encapsulating molecular condensates

Molecular crowding is an inherent feature of the cell interior. Synthetic cells as provided by giant unilamellar vesicles (GUVs) encapsulating macromolecules (polyethylene-glycol and dextran) represent an excellent mimetic system to study membrane transformations associated with molecular crowding and protein condensation. Similarly to cells, such GUVs loaded with macromolecules exhibit highly curved structures such as internal nanotubes. In addition, upon liquid-liquid phase separation as inside living cells, the membrane of GUVs encapsulating an aqueous two-phase system deforms to form apparent kinks at the contact line of the interface between the two aqueous phases. These structures, nanotubes and kinks, have dimensions below optical resolution and if resolved, can provide information about material properties such as membrane spontaneous curvature and intrinsic contact angle describing the wettability contrast of the encapsulated phases to the membrane. Previous experimental studies were based on conventional optical microscopy which cannot resolve these membrane and wetting proper-ties. Here, we studied these structures with super-resolution microscopy, namely stimulated emission depletion (STED) microscopy, together with microfluidic manipulation. We demonstrate the cylindrical nature of the nanotubes with unprecedented detail based on the superior resolution of STED and automated data analysis. The spontaneous curvature deduced from the nanotube diameters is in excellent agreement with theoretical predictions. Furthermore, we were able to resolve the membrane 'kink' structure as a smoothly curved membrane demonstrating the existence of the intrinsic contact angle. We find very good agreement between the directly measured values and the theoretically predicted ones based on the apparent contact angles on the micrometer scale. During different stages of cellular events, biomembranes undergo a variety of shape transformations such as the formation of buds and nanotubes regulated by membrane necks. We demonstrate that these highly curved membrane structures are amenable to STED imaging and show that such studies provide important insights in the membrane properties and interactions underlying cellular activities.

Controlling states of water droplets on nanostructured surfaces by design

The transition between the Cassie and Wenzel states can be controlled via precisely designed trapezoidal nanostructures on the surface, for which the base angle of the trapezoids and the intrinsic contact angle of the surface are two possible adjustable parameters.

Effect of nano structures on the nucleus wetting modes during water vapour condensation: from individual groove to nano-array surface

The geometrical structures of surfaces are important to the formation and growth of nuclei during water vapour condensation. Nucleus wetting modes on grooved surfaces are determined by the intrinsic contact angle and the cross sectional angle.

Dynamic Behaviors and Energy Transition Mechanism of Droplets Impacting on Hydrophobic Surfaces

The wettability of hydrophobic surfaces and the dynamic behaviors of droplets impacting on hydrophobic surfaces are simulated using a lattice Boltzmann method, and the condition for the rebound phenomenon of droplets impacting on solid surfaces is analyzed. The results show that there is a linear relationship between the intrinsic contact angle and the interaction strength of fluid-wall particles. For hydrophobic surfaces with the same intrinsic contact angle, the micromorphology can increase the surface hydrophobicity, especially the hierarchical micromorphology. The dynamic behaviors of droplets impacting on solid surfaces are affected by the wettability. The surface hydrophobicity is stronger, and the rebound phenomenon occurs easier. If the droplet’s kinetic energy is greater than the sum of the surface energy and the minimum conversion gravitational potential energy when the spreading and shrinking finish, the rebound phenomenon will occur. As the hydrophobic surface’s viscous dissipation is much smaller than the hydrophilic surface’s, the droplet still has high kinetic energy after the spreading and shrinking, which is advantageous to rebound for droplets.

Effect of Materials’ Wettability on the Dynamic Behavior of Droplet Impact onto a Rough Solid Surface

In this paper, the effect of material’s wettability on the droplet impact has been investigated by numerical apporach. The unsteady flow behaviors of liquid droplet impacting against the rough solid surface with different wettabilities have been simulated based on lattice Boltzmann method. The spreading and bounding characterisitcs of droplet have been discussed. For the hydrophilic material, the droplet will sink into the grooves among roughness bumps, and its apparent contact angle in steady stead will be smaller than its corresponding intrinsic contact angle; while for the hydrophilic material, droplet will flow into the grooves but suspend on the top of roughness elements without any contacting with the bottom surface, and the apparent contact angle is larger than its intrinsic contact angle.