CRITICAL CARE & THE EARLY WEB: ETHICAL DIGITAL METHODS FOR ARCHIVED YOUTH DATA

This paper demonstrates an ethico-methodological approach to researching archived web pages created by young people throughout 1994-2005 that was collected and stored by the Internet Archive. Rather than deploying a range of computational tools available for collecting web data in the Internet Archive, my approach to this material has been to start with the person: I recruited participants through social media who remembered creating websites or participating in web communities when they were younger and were interested in attempting to relocate their digital traces. In a series of qualitative, online semi-structured interviews, I guided participants through the Wayback Machine’s interface and directed them towards where their materials might be stored. I adapted this approach from the walkthrough method, where I position the participant as co-investigator and analyst of web archival material, enabling simultaneous discovery, memory, interpretation and investigation. Together, we walk through the abandoned sites and ruins of a once-vibrant online community as they reflect and remember the early web. This approach responds to significant ethical gaps in web archival research and engages with feminist ethics of care (Luka & Millette, 2018) inspired by conceptual framing of data materials in research on the "right to be forgotten” (Crossen-White, 2015; GDPR, 2018; Tsesis, 2014), digital afterlives (Sutherland, 2020), indigenous data sovereignty and governance (Wemigwans, 2018), and the Feminist Data Manifest-No (Cifor et al, 2019). This method re-centers the human and moves towards a digital justice approach (Gieseking, 2020; Cowan & Rault, 2020) for engaging with historical youth data.

50th anniversary of the discovery of reverse transcriptase

The simultaneous discovery in 1970 of reverse transcriptase in virions of retroviruses by Howard Temin and David Baltimore was perhaps the most dramatic scientific moment of the second half of the 20th century. Ten years previously, Temin’s observation of cells transformed by Rous Sarcoma virus led him to the conclusion that retroviruses replicate through a DNA intermediate he called the provirus. This heretical hypothesis was greeted with derision by fellow scientists; Temin and Baltimore performed a simple experiment, rapidly reproduced, and convincing to all. Its result was a major paradigm shift—reversal of the central dogma of molecular biology. It immediately grabbed the attention of both the scientific and lay press. It also came at a key time for cancer research, at the start of the “War on Cancer.” As a theoretical base and fundamental molecular tool, it enabled a decade of (largely fruitless) search for human oncogenic retroviruses but laid the foundation for the discovery of HIV 13 years later, leading to the development of effective therapy. I had the good fortune, as a student in Temin’s lab, to witness these events. I am honored to be able to share my recollection on the occasion of their 50th anniversary.

Simultaneous Discovery of Positive and Negative Interactions Among Rhizosphere Bacteria Using Microwell Recovery Arrays

Understanding microbe-microbe interactions is critical to predict microbiome function and to construct communities for desired outcomes. Investigation of these interactions poses a significant challenge due to the lack of suitable experimental tools available. Here we present the microwell recovery array (MRA), a new technology platform that screens interactions across a microbiome to uncover higher-order strain combinations that inhibit or promote the function of a focal species. One experimental trial generates 104 microbial communities that contain the focal species and a distinct random sample of uncharacterized cells from plant rhizosphere. Cells are sequentially recovered from individual wells that display highest or lowest levels of focal species growth using a high-resolution photopolymer extraction system. Interacting species are then identified and putative interactions are validated. Using this approach, we screen the poplar rhizosphere for strains affecting the growth of Pantoea sp. YR343, a plant growth promoting bacteria isolated from Populus deltoides rhizosphere. In one screen, we montiored 3,600 microwells within the array to uncover multiple antagonistic Stenotrophomonas strains and a set of Enterobacter strains that promoted YR343 growth. The later demonstrates the unique ability of the platform to discover multi-membered consortia that generate emergent outcomes, thereby expanding the range of phenotypes that can be characterized from microbiomes. This knowledge will aid in the development of consortia for Populus production, while the platform offers a new approach for screening and discovery of microbial interactions, applicable to any microbiome.

The Origins of the CES Production Function

The CES production function was introduced to economics in the 1961 paper “Capital-Labor Substitution and Economic Efficiency,” by Kenneth Arrow, Hollis Chenery, Bagicha Minhas, and Robert Solow. The paper had an immediate and substantial impact on economic research, and the CES production function remains an important tool for both theoretical and empirical researchers. I review how the CES production function was derived and used in the paper, and, relying on archival sources, present a fine-grained account of the collaborative process that produced the paper. I also discuss the CES production function as an example of multiple simultaneous discovery and suggest reasons for its broad and rapid diffusion.

Eunice Foote, John Tyndall and a question of priority

In 1856, an American woman, Eunice Foote, discovered the absorption of thermal radiation by carbon dioxide and water vapour. That was three years before John Tyndall, who is generally credited with this important discovery—a cornerstone of our current understanding of the greenhouse effect, climate change, weather and meteorology. Tyndall did not reference Foote's work. From a contemporary perspective, one might expect that Tyndall would have known of her findings. But it appears that he did not, raising deeper historical questions about the connections and relationships between American and European physicists in the mid nineteenth century. The discovery is seen as a significant moment in physics generally and in climate science in particular, and demands a proper analysis. This paper explores the argument about priority, and the issues that the episode highlights in terms of simultaneous discovery, the development of science in America, gender, amateur status, the reputation of American science in Europe and the networks and means of communication between researchers in America and Europe in the 1850s.