Strengthening of Subgrade Clayey Soil in Road Construction using Fly ash and Coir Geotextile

The best handicap for a carried-out community of road structures in growing international locations like India is the restricted monetary assets accessible to construct roads the use of traditional methods. By the usage of nearby substances inclusive of neighbourhood grounds for the constructions of the lower layers of the pavements (in precise the subgrade), the development expenses can be extensively reduced. 20% of the land in India is clayey and is expansive in nature. These lands are observed to be steeply-priced to construct and to maintain roads. The use of coir fibre substances in the discipline of civil engineering has led to new methods for stabilisation of soils in particular. A coir fibre (CF) is an herbal cloth that is broadly handy in Coastal India. A certain find out about was carried out in this paper about enhancing the stability, energy and sturdiness of soil clay mixed up with fly ash and coir fibre mat. The sample of the soil used was from the excessive clay region in Andhra Pradesh. The stabilisation was performed with classification C fly ash and grade H2M9 coir mat. The plasticity of clay fly ash mixes is decreased as fly ash content material is increased. Adding fly ash consequently lessens increasing soils and will increase their working-ability via a colloidal response and adjustments in grain size. The supplementation of fly ash led to full-size increase in soil CBR. The consequences exhibit large enhancement in compaction and CBR of composite containing clay, fly ash and coir mat. The CBR value for virgin Andhra Pradesh clayey soil was 6% which improved to 12% for optimal fly ash (15%) –clayey mix. The CBR value was determined through placing coir mat at more than a few depths in ideal fly ash clay mix. The most CBR value acquired was 44% for coir mat positioned at mixture of h/4th and h/2th depth from pinnacle in standard fly ash - clay mix.

Cocrystals of Ethenzamide With 2-Nitrobenzoic Acid - Conformational Analysis, MD Simulations And DFT Investigations

In crystal engineering and pharmaceutical chemistry, cocrystals have a wide range of applications. Ethenzamide (EA) is found to form cocrystal with 2-nitrobenzoic acid (NBA). Geometry properties like stability energy, charge distribution, bond length, electronic properties and thermodynamic characteristics have been analyzed. The C-H…O hydrogen bond involves C-H of EA and oxygen of NBA. Configuration with the angle, N3-C4-C5-C6 gives the lowest energy conformation. Partition coefficient value suggests that EA-NBA has pharmaceutics behavior. RMSD values show the simulation’s relative stability and the complexes, remained stable throughout.

Improved TCP Prediction Congestion in Mobile Ad Hoc Network Based on Cross-Layer and Fuzzy Logic

Mobile ad hoc network (MANET) is among the networks which do not require any infrastructure to put nodes in communication. Due to its own nature, it is used by several applications. Even though it's a network that is extremely challenging and mostly when TCP is applied. In this paper, we have proposed a new improvement in the TCP algorithm that employed fuzzy logic to predict packet loss and avoid congestion. Specifically, we have used tree metrics such as stability, energy, and signal strength to use in fuzzy logic systems. To accomplish our approach, we have established some modifications based on a cross-layer. The results of the relevant simulation performed by NS3 demonstrated that our approach globally improves the performance of TCP in MANET. Precisely reduce the packet overhead and energy consumption also enhance throughput.

Phosphorylation Dependent Sequestration Of Protein Phosphatase-1 I (PP-1 I) By Protein 14-3-3

PP-1 is involved in the control of the meiotic and mitotic cell divisions at the G2 to M transition by dephosphorylating phospho-serine 217 (phospho-serine 287 in Xenopus) of Cdc25, accompanied by activation of Cdc25 which would then dephosphorylate and activate Cdk1 . PP-1I has been proposed to be the form of PP-1 that dephosphorylates and activates Cdc25. Here we show that PP-1I is sequestered in a phosphorylation dependent manner by protein 14-3-3 involving phosphorylation of serine 71 of I-2 moiety of PP-1I by C-TAK1. Phosphorylation of serine 71 of the I-2 moiety of PP-1I by C-TAK1 enhanced the sequestration and inhibition of PP-1I by Protein 14-3-3. Phosphorylation of serine 71 of the I-2 moiety of PP-1I caused an increase in the Stability Energy, Binding Energy of the interaction between PP-1I and Protein 14-3-3 and a significant decrease in the IC50 of the inhibition of PP-1I by Protein 14-3-3. PP-1I regulation by C-TAK1 and Protein 14-3-3 is a mechanism for the control of Cdc25 at the G2 to M transition of the cell cycle.

Conformational Stability, FMO, NMR, MEP and NBO Analysis of 2,5-Di-methyl-2,5-bis(methylthio)-1,4-dithiane and Dimethoxy compounds by DFT Approach

Conformational behaviors of 2,5-dimethoxy-2,5-dimethyl-1,4-dithiane (compound 1) and 2,5-dimethyl-2,5-bis (methylthio)-1,4-dithiane (compound 2) investigated by computational methods including B3LYP/6-311+G** and M06-2X/6-311+G** levels of theory and NBO analysis. The stereoelectronic effect of axial, axial (ax, ax) and equatorial, equa-torial (eq, eq) conformations were studied using NBO analysis. Using NBO analysis, the values of the stereoelectronic effects were calculated through the energy of stability associated with the electron transfers of compounds 1 and 2. The results showed that the eq, eq conformations of the studied compounds were more stable than their corresponding ax, ax conformations, and LP2X→σS1-C2 and LP2S→σ*C2-X electron transfers play important roles in the conformational be-havior of the studied compounds. The main purpose of the present work was to study the effects of stereoelectronic inter-actions and steric on the conformational superiority of the di-methoxy (compound 1) and di-thiomethyl compounds (com-pound 2). Thus, the values of resonance stability energy, non-diagonal elements, and orbital populations were investigated. Also, active electrophilic and nucleophilic centers were identified using fronting orbitals analysis obtained by DFT methods. The electrostatic potential maps of the title compounds were investigated at the B3LYP/6-311+G* level of theory. All of the NMR parameters and geometrical properties of both compounds were determined in this study.

Structure Model Analysis of the Effects of Mutations in the Phosphorylation Sites of SARS-CoV-2 Encoded Nucleocapsid Protein

Mutations in several phosphorylation sites within the phosphorylation rich domain of SARS-COV-2 Nucleocapsid protein (NCp), including serines 186, 197 and 202, and adjacent arginine 203 and glycine 204 have been described and have been proposed to prevent the binding and sequestration of NCp by Protein 14-3-3. Structure modeling and thermodynamic calculation show that mutations of phosphorylation sites, phospho-serines 186, 197 and 202 to phenylalanine, leucine and asparagine, and phosphorylation recognition sites, arginine/glycine 203/204 to lysine/arginine or lysine/threonine resulted in signifi cant destabilization of the NCp-14-3-3 complex by causing a decrease in Stability Energy (ΔGstability energy) and Binding Energy (ΔΔGbinding energy). These results evidenced that mutations in NCp underlie a mechanism to bypass sequestration by Protein 14-3-3 which would result in enhanced dimerization of NCp, and replication, transcription and packaging of the SARS-COV-2 genome

Structure Model Analysis Of Phosphorylation Dependent Binding And Sequestration Of SARS-COV-2 Encoded Nucleocapsid Protein By Protein 14-3-3

Phosphorylation of serines 197 and 206 of SARS-COV-2 Nucleocapsid protein (NCp) enhanced the stability and binding efficiency and sequestration of NCp to Protein 14-3-3 by increasing the Stability Energy (ΔGstability energy) and Binding Energy (ΔΔGbinding energy) from ~545 Kcal/mol to ~616 Kcal/mol, and from 108 Kcal/mol to ~228 Kcal/mol respectively. The calculated Binding Energy Difference (ΔΔGbinding energy difference) between dephospho-NCp-14-3-3 complex and phospho-NCp-13-3-3 complex was ~72 Kcal/mol. Phosphorylations of serines 186, 197, 202 and 206, and threonines 198 and 205 NCp also caused an increase in the Stability Energy (ΔGstability energy) and Binding Energy (ΔΔGbinding energy) from ~545 Kcal/mol to ~617, 616, 583, 580, 574, 564 and 566 Kcal/mol and from ~108 Kcal/mol to ~228, 216, 184, 188, 184, 174 and 112 Kcal/mol respectively. Phosphorylation of NCp on serines 197 and 206 caused a decrease in Stability Energy and Binding Energy from ~698 Kcal/mol to 688 Kcal/mol, and from ~91 Kcal/mol to ~82 Kcal/mol for the dimerization of NCp. These results support the existence of a phosphorylation dependent cellular mechanism to bind and sequester NCp.

Role of Different Integer Virtual Machine in Cloud Data Center

In modern data centres of cloud computing contains virtualization system. In order to improve network stability, energy efficiency, and makespan proper virtualization need. The virtual machine is one of the examples of virtualizations. Cloud computing data centres consist of millions of virtual machine to manage load balancing. In this study check the different number of virtual machine role in data centres, for that purpose, we established a network with the help of cloudsim and compare different data centres at each zones taking a different number of the virtual machine with different paramater and network banwith.After the simulation the result shows that increasning in the number of VM can affect the netwok accuracy in term of energy ,processing time ,coast and network stabality .