Associations between polymorphisms of SLC22A7, NGFR, ARNTL and PPP2R2B genes and Milk production traits in Chinese Holstein

Background Our preliminary work confirmed that, SLC22A7 (solute carrier family 22 member 7), NGFR (nerve growth factor receptor), ARNTL (aryl hydrocarbon receptor nuclear translocator like) and PPP2R2B (protein phosphatase 2 regulatory subunit Bβ) genes were differentially expressed in dairy cows during different stages of lactation, and involved in the lipid metabolism through insulin, PI3K-Akt, MAPK, AMPK, mTOR, and PPAR signaling pathways, so we considered these four genes as the candidates affecting milk production traits. In this study, we detected polymorphisms of the four genes and verified their genetic effects on milk yield and composition traits in a Chinese Holstein cow population. Results By resequencing the whole coding region and part of the flanking region of SLC22A7, NGFR, ARNTL and PPP2R2B, we totally found 20 SNPs, of which five were located in SLC22A7, eight in NGFR, three in ARNTL, and four in PPP2R2B. Using Haploview4.2, we found three haplotype blocks including five SNPs in SLC22A7, eight in NGFR and three in ARNTL. Single-SNP association analysis showed that 19 out of 20 SNPs were significantly associated with at least one of milk yield, fat yield, fat percentage, protein yield or protein percentage in the first and second lactations (P < 0.05). Haplotype-based association analysis showed that the three haplotypes were significantly associated with at least one of milk yield, fat yield, fat percentage, protein yield or protein percentage (P < 0.05). Further, we used SOPMA software to predict a SNP, 19:g.37095131C > T in NGFR, changed the structure of NGFR protein. In addition, we used Jaspar software to found that four SNPs, 19:g.37113872C > G,19:g.37113157C > T, and 19:g.37112276C > T in NGFR and 15:g.39320936A > G in ARNTL, could change the transcription factor binding sites and might affect the expression of the corresponding genes. These five SNPs might be the potential functional mutations for milk production traits in dairy cattle. Conclusions In summary, we proved that SLC22A7, NGFR, ARNTL and PPP2R2B have significant genetic effects on milk production traits. The valuable SNPs can be used as candidate genetic markers for genomic selection of dairy cattle, and the effects of these SNPs on other traits need to be further verified.

Effect of the Vermicompost and Poultry Manure on Groundnut (Arachis hypogaea L.) under Rain-fed Condition

Background: Use of organic manures to meet the nutrient requirement of the crop would be an inevitable practice for sustainable agriculture. Since organic manures generally improve the physical, chemical and biological properties along with conserving soil moisture and thus resulting in enhanced crop productivity along with maintaining the quality of crop production. Organic farming in recent years is gaining significance due to the realization of inherent advantages it confers in sustaining crop production and also in maintaining dynamic soil nutrient status and safe environment. Organic farming is also concerns to produce the quality food and human health, so that this research and studied the interaction effect of vermicompost and poultry manure on groundnut crop in Chitrakoot condition. Methods: The experiment was conducted at Rajola Farm of the Faculty of Agricultural Sciences, Mahatma Gandhi Chitrakoot Gramodaya Vishwavidyalaya, Chitrakoot in kharif 2019. The present experiment was conducted to evaluate the effect of different levels of poultry manure and vermicompost applications. The morphological and biochemical parameters were evaluated in experimental farm and departmental laboratory. Result: The combination dose of V3P3 (6 ton ha-1 vermicompost and 4 ton ha-1 poultry manure) was found to be best giving the maximum values. The effect of V3 on the number of pod per plant and number of seed per plant was maximum 15.20 and 3.22 and effect of P3 on the number of pod per plant and number of seed per plant was maximum 14.156 and 3.11 respectively. The interaction of vermicompost and poultry manure of the V3P3 combination was recorded maximum 17.13 per plant. Seed per pod interaction of vermicompost and poultry manure of the V3P3 combination also recorded maximum 3.66 per pod. Effect of vermicompost and poultry manure on 100 kernel weight was maximum in V3 (42.55 g) and P3 (41.00 g) respectively. The shelling percentage also was recorded maximum in V3 (69.77) and P3 (67.77) and interaction of both parameters was recorded non significance. Effect on yield (q/ha) was recorded maximum in V3 (1.05) and P3 (0.99) and the interaction was recorded maximum in V3P3 combination (1.27). The effect of vermicompost on oil percentage, protein percentage and oil yield (kg/ha) was recorded maximum 48.46, 21.33 and 1573.5 in V3 respectively. The effect of poultry manure recorded maximum in P3 (48.03), 20.68 and 1411.2 kg/ha oil percentage, protein percentage and oil yield respectively. The interaction of vermicompost and poultry manure was recorded maximum with the combination of V3P3 and found 49.52 oil percentage, 21.76 protein percentage and 1696.00 kg/ha oil yield. The results indicated that all these parameters were significantly increased due to vermicompost and poultry manure application. Their interaction was also significant. It was evident that a combination dose of V3P3 (6 ton ha-1 vermicompost and 4 ton ha-1 poultry manure) was found to be best giving the maximum values.

Physical Characteristics and Proximate Composition of Three Commercial Shrunken 2 Varieties of Sweet Corn in the Philippines as Influenced by Harvest Maturity

The physical and proximate characteristics of three commercial varieties of shrunken 2 sweet corn as affected by harvest maturities (18, 20 and 22 days after 100% silking) were evaluated. As sweet corn, there was a significant increase in length in SC1 and SC2. On the other hand all varieties ear diameter increased with maturity. The three varieties have significant differences in ear length and diameter. Lightness of kernels was not affected by maturity and varietal differences. Increasing a* and b* values suggested that the kernels became more red and yellow as they mature on the plant due to carotenogenesis. The a* and b* values of SC1 and SC2 were significantly affected by maturity while SC3 was not. In terms of chroma, only SC2 was significantly affected by harvest maturity, with an increased in color vividness as it matured in the plant. While hue values of the three varieties were not affected by harvest maturity. SC1 and SC2 kernel percentage moisture decreased significantly as sweet corn matures in the plant. Percentage ash content was not significantly affected by harvest maturity. On the other harvest maturity affected SC1 and SC2 percentage protein, but not their fiber content. The percentage fat and nitrogen free extract of SC1 kernel decreased as the plant matures. Mean energy content was significantly different with harvest maturity in all three varieties at a P value < 0.01 (SC1 and SC2) and P = 0.05 (SC3). Harvest maturity affected the physical and proximate composition of the three commercial varieties of shrunken 2 sweet corn in the Philippines.

REALIZATION OF PARENTAL INDICES OF COW PRODUCTIVITY IN DIFFERENT MILKING BREEDS AT BREEDING FARM "AGROFIRMA "PAKHMA"

Combination of genotypes of main types’ genetic markers causes milk productivity in cattle. The article studies correlation between kappa casein genotypes (C3N3), beta-lactoglobulin (LGB), their complexes and milk production indices in cows bred under equal conditions at a breeding farm. The calculation of parental milk yield and fat indices between the three breeds showed a significant difference (P>0,999) of PCI of Ayrshire and Holstein cattle breed milk yield - 3395 kg, PCI of fat - 0,22%; between Holstein and Yaroslavl cattle breeds - 3200 kg, PCI offat - 0,16%. A significant difference of realization indices of protein between all three breeds was not stated. The highest realization ofparental milk yield index was stated - between Ayrshire and Yaroslavl cattle breeds- 113%, Holstein cattle breed - 88%. As for the fat and protein indices, all three cattle breeds showed a high realization of PCI from 98% to 109%. However, Holstein cattle breed showed the highest percentage: protein - 101%, fat - 109%. A significant difference of PCI realization of milk productivity in CSN3 and LGB genotypes was not stated. It was also stated that there is a tendency of high level parental indices realization when there is a higher number of B-allele variants in genotypes of cows.

Nutritional Profile and Organoleptic Qualities of Milk Chocolate Incorporated with Different Spices

An innovative spicy chocolate was developed in this study by partial replacement of cocoa nibs with different selected spice powders of: Ginger (G11), Garlic (G12), Clove (C13), Cinnamon, (C14), Turmeric (T15), Aframomum danielli (A16), Aframomum melegueta (A17), Thyme (T18), Black Pepper (B19) and Clappertonia (C20). Milk chocolate without spices (C10) served as Control. The proximate compositions were carried out using standard methods. The Total Calorific values (TCV) was determined using the Atwater factors (physiological fuel values) of 4kcal, 4kcal and 9kcal per gram of carbohydrate, protein and fat respectively. The sensory evaluation was carried out by panel of tasters consisting of 13 males and 18 female staff of the Cocoa Research Institute of Nigeria who were used to consuming chocolates. The result showed significant differences in the proximate chemical compositions of the chocolates (p<0.05). Apart from black pepper with a An significantly lower protein and ash contents when compared to the control chocolate, all the other spices significantly caused an increase in percentage protein and ash in the control milk chocolate. The trend of protein increase is T18 > G11 > T15 > C20 =A17 > A16 > C14 > C13 > G12 > C10 > B19 > respectively. The range of values obtained for the proximate compositions were (%)The Total Calorific Value (TCV) also showed significant differences among all the samples at p<0.05 with the TCV of the control chocolate being the highest. The addition of spices contributed to acceptable tastes, aroma, flavor and general acceptability of milk chocolates. In Conclusion, this study showed that the incorporation of Spices in chocolate increased the protein and ash content of milk chocolate, the organoleptic acceptance while reducing the TCV values This is a desirable findings in view of the fear of high calorific values of Milk Chocolate which may predispose consumers to Obesity when consumed.in large quantities.

Effect of Ionic Strength, pH and Chemical Displacers on the Percentage Protein Binding of Protein-Bound Uremic Toxins

Background and Objectives: While trying to optimize the dialysis clearances of protein-bound uremic toxins (PBUTs), their percentage protein binding (% PB) is an important parameter. We evaluated the effects of ionic strength, pH change and chemical displacers on the dissociation of PBUTs from albumin in vitro. Methods: PBUTs, such as 3-Carboxy-4-methyl-5-propyl-2-furan-propanoic acid (CMPF), p-cresylsulfate (PCS), indoxyl sulfate (IS) and indole-3-acetic acid (IAA), were spiked with human serum albumin (HSA) solution prepared with different Nacl concentrations and pH values or in the presence of a series of chemical displacers. Ultrafiltration was performed to separate the free and bound fractions, and the % PB of each PBUT was calculated. Results: For all 4 compounds, their % PB decreased with increasing ionic strength, while only slight changes occurred when the pH of the test solution increased from pH 6.0 to pH 8.5; PCS, IS and 3-IAA were relatively easily dissociated from albumin by drug displacement, while CMPF was released from HSA by all studied drugs with difficulty; the PB % for CMPF, PCS, IS and 3-IAA decreased most remarkably in the presence of free fatty acids, such as oleic acid (41.73% for CMPF, 29.9% for PCS, 23.22% for IS, and 20.34% for 3-IAA) and linoleic acid (43.12% for CMPF, 16.65% for PCS, 29.99% for IS, and 16.29% for 3-IAA). Conclusion: The protein binding of PBUTs can be decreased by higher ionic strength, increased pH and the presence of some chemical displacers, including free fatty acids. Effective dialytic removal of PBUTs may be achieved by applying these methods jointly to blood-purification techniques.

Phenomenological Model of Percentage Protein in fermented Iru (Parkia biglobosa)

The optimum conditions for the fermentation of African locust bean (Parkia biglobosa) into a vegetable protein based condiment (Iru) were developed using Levenberg-Marquardt (or Powell) method (using PSI software) with three (3) variables namely; inoculum concentration (bacillus subtilis), temperature and the fermentation duration. African locust bean seeds were fermented at various temperature of 40 - 70 &deg;С for five days (120 hours) with different concentrations of Inoculum. The proximate analysis shows that fermentation increased the percentage protein. Protein had the highest composition with about 51 % after 72 hours at the lowest fermentation temperature of 40&deg;С.

Effect of sample temperature, pH, and matrix on the percentage protein binding of protein-bound uraemic toxins

While studying and trying to optimise dialysis clearances of protein-bound uraemic toxins (PBUTs), the percentage protein binding (% PB) may be an important parameter and can be calculated from measured free and total concentrations.

Application of Experimental Design in Preparation of Nanoliposomes Containing Hyaluronidase

Hyaluronidase is an enzyme that catalyzes breakdown of hyaluronic acid. This property is utilized for hypodermoclysis and for treating extravasation injury. Hyaluronidase is further studied for possible application as an adjuvant for increasing the efficacy of other drugs. Development of suitable carrier system for hyaluronidase would help in coadministration of other drugs. In the present study, the hyaluronidase was encapsulated in liposomes. The effect of variables, namely, phosphatidylcholine (PC), cholesterol, temperature during film formation (T1), and speed of rotation of the flask during film formation (SPR) on percentage of protein encapsulation, was first analyzed using factorial design. The study showed that level of phosphatidylcholine had the maximum effect on the outcome. The effect of interaction of PC and SPR required for preparation of nanoliposomes was identified by central composite design (CCD). The dependent variables were percentage protein encapsulation, particle size, and zeta potential. The study showed that ideal conditions for production of hyaluronidase loaded nanoliposomes are PC—140 mg and cholesterol 1/5th of PC when the SPR is 150 rpm and T1 is 50°C.