Dissecting cascade computational components in spiking neural networks

Finding out the physical structure of neuronal circuits that governs neuronal responses is an important goal for brain research. With fast advances for large-scale recording techniques, identification of a neuronal circuit with multiple neurons and stages or layers becomes possible and highly demanding. Although methods for mapping the connection structure of circuits have been greatly developed in recent years, they are mostly limited to simple scenarios of a few neurons in a pairwise fashion; and dissecting dynamical circuits, particularly mapping out a complete functional circuit that converges to a single neuron, is still a challenging question. Here, we show that a recent method, termed spike-triggered non-negative matrix factorization (STNMF), can address these issues. By simulating different scenarios of spiking neural networks with various connections between neurons and stages, we demonstrate that STNMF is a persuasive method to dissect functional connections within a circuit. Using spiking activities recorded at neurons of the output layer, STNMF can obtain a complete circuit consisting of all cascade computational components of presynaptic neurons, as well as their spiking activities. For simulated simple and complex cells of the primary visual cortex, STNMF allows us to dissect the pathway of visual computation. Taken together, these results suggest that STNMF could provide a useful approach for investigating neuronal systems leveraging recorded functional neuronal activity.

Analysis, Simulation, and Development of a Low-Cost Fully Active-Electrode Bioimpedance Measurement Module

A low-cost 1 kHz–400 kHz operating frequency fully-active electrode bioimpedance measurement module, based on Howland current source, is presented in this paper. It includes a buffered positive feedback Howland current source, implemented with operational amplifiers, as well as an AD8421 instrumentation amplifier, for the differential voltage measurements. Each active electrode module can be connected to others, assembling a wearable active electrode module array. From this array, 2 electrodes can be selected to be driven from a THS413 fully differential amplifier, activating a mirrored Howland current source. This work performs a complete circuit analysis, verified with MATLAB and SPICE simulations of the current source’s transconductance and output impedance over the frequency range between 1 kHz and 1 MHz. Resistors’ tolerances, possible mismatches, and the operational amplifiers’ non-idealities are considered in both the analysis and simulations. A comparison study between four selected operational amplifiers (ADA4622, OPA2210, AD8034, and AD8672) is additionally performed. The module is also hardware-implemented and tested in the lab for all four operational amplifiers and the transconductance is measured for load resistors of 150 Ω, 660 Ω, and 1200 Ω. Measurements showed that, using the AD8034 operational amplifier, the current source’s transconductance remains constant for frequencies up to 400 KHz for a 150 Ω load and 250 kHz for a 1200 Ω load, while lower performance is achieved with the other 3 operational amplifiers. Finally, transient simulations and measurements are performed at the AD8421 output for bipolar measurements on the 3 aforementioned load resistor values.

CFPU: A Cell-Free Processing Unit for High-Throughput, Automated In Vitro Circuit Characterization in Steady-State Conditions

Forward engineering synthetic circuits are at the core of synthetic biology. Automated solutions will be required to facilitate circuit design and implementation. Circuit design is increasingly being automated with design software, but innovations in experimental automation are lagging behind. Microfluidic technologies made it possible to perform in vitro transcription-translation (tx-tl) reactions with increasing throughput and sophistication, enabling screening and characterization of individual circuit elements and complete circuit designs. Here, we developed an automated microfluidic cell-free processing unit (CFPU) that extends high-throughput screening capabilities to a steady-state reaction environment, which is essential for the implementation and analysis of more complex and dynamic circuits. The CFPU contains 280 chemostats that can be individually programmed with DNA circuits. Each chemostat is periodically supplied with tx-tl reagents, giving rise to sustained, long-term steady-state conditions. Using microfluidic pulse width modulation (PWM), the device is able to generate tx-tl reagent compositions in real time. The device has higher throughput, lower reagent consumption, and overall higher functionality than current chemostat devices. We applied this technology to map transcription factor-based repression under equilibrium conditions and implemented dynamic gene circuits switchable by small molecules. We expect the CFPU to help bridge the gap between circuit design and experimental automation for in vitro development of synthetic gene circuits.

CFPU: a cell-free processing unit for high-throughput, automated in vitro circuit characterization

Forward engineering synthetic circuits is at the core of synthetic biology. Automated solutions will be required to facilitate circuit design and implementation. Circuit design is increasingly being automated with design software, but innovations in experimental automation are lagging behind. Microfluidic technologies made it possible to perform in vitro transcription-translation (tx-tl) reactions with increasing throughput and sophistication, enabling screening and characterization of individual circuit elements and complete circuit designs. Here we developed an automated microfluidic cell-free processing unit (CFPU) that extends high-throughput screening capabilities to a continuous reaction environment, which is essential for the implementation and analysis of more complex and dynamic circuits. The CFPU contains 280 chemostats that can be individually programmed with DNA circuits. Each chemostat is periodically supplied with tx-tl reagents, giving rise to sustained, long-term steady state conditions. Using microfluidic pulse width modulation (PWM) the device is able to generate tx-tl reagent compositions in real-time. The device has higher throughput, lower reagent consumption, and overall higher functionality than current chemostat devices. We applied this technology to map transcription factor based repression under equilibrium conditions and implemented dynamic gene circuits switchable by small molecules. We expect the CFPU to help bridge the gap between circuit design and experimental automation for in vitro development of synthetic gene circuits.

Application of Innovative Processes for Gold Recovery from Romanian Mining Wastes

The application of a new hydrometallurgical process for gold extraction by thiosulphate leaching from Romanian mining wastes, coming from Balan and Deva deposits, was studied. There was obtained 85% of Au extraction after leaching; moreover, an integrated flow-sheet, including recycling of process solution and carbon, was outlined, based on results obtained at a laboratory scale, using a schematic chemical circuit of treatment. Global recovery of the process (leaching-adsorption-desorption-electrodeposition) of about 75-80% of Au was achieved. The developed integrated flow-sheet, allows to recycle the reagents during the process, with a loss of only 5-10%, in particular thiosulphate and alcohol, for each complete circuit of treatment.

Near-optimal rotation of colour space by zebrafish cones in vivo

For colour vision, retinal circuits must separate information about intensity and wavelength. This requires circuit-level comparison of at least two spectrally distinct photoreceptors. However, many vertebrates use four or more, and in those cases the nature and implementation of this computation remains poorly understood. Here, we establish the complete circuit architecture and function of outer retinal circuits underlying colour processing in the tetrachromatic larval zebrafish. Our findings reveal that the specific spectral tunings of the four cone types near optimally rotate the encoding of natural daylight in a principal component analysis (PCA)-like manner to yield one primary achromatic axis, two colour-opponent axes as well as a secondary UV-achromatic axis for prey capture. We note that fruit flies – the only other tetrachromat species where comparable circuit-level information is available - use essentially the same strategy to extract spectral information from their relatively blue-shifted terrestrial visual world. Together, our results suggest that rotating colour space into achromatic and chromatic axes at the eye’s first synapse may be a fundamental principle of colour vision when using more than two spectrally well-separated photoreceptor types.

Circuit Determinants of Ventricular Tachycardia Cycle Length: Characterization of Fast and Unstable Human VT

Background: Fast ventricular tachycardias (VTs) have been historically attributed to shorter path lengths with smaller reentrant circuit dimensions in animal models. The relationship between the dimensions of the reentrant VT circuit and tachycardia cycle length (TCL) has not been examined in humans. This study aimed to analyze the determinants of the rate of human VT with comparison of circuit dimensions and conduction velocity (CV) across a wide range of both stable and unstable VTs delineated by high-resolution mapping. Methods: 54 VTs with complete circuit delineation (>90% TCL) by high-resolution multielectrode mapping were analyzed in 49 patients (male 88%, 65 years [58-71], nonischemic 47%). Fast VT was defined as TCL <333 ms (rate >180 bpm). Unstable VT was defined by hemodynamic deterioration with an intrinsic mean arterial pressure <60 mmHg during a sustained episode. Results: The median TCL of VT was 365 ms (306-443 ms) and 24 fast VTs with TCL<333ms (180 bpm) were characterized. A wide range of CV was observed within the entrance (0.03-0.55 m/s), common pathway (0.03-0.77 m/s), exit (0.03-0.53m/s), and outer loop (0.17-1.13 m/s). There were no significant differences in the median dimensions of the isthmus and path length between fast versus slow VTs and unstable versus stable VTs. The outer loop CV was the only circuit component that correlated with TCL both in ischemic cardiomyopathy (r=-0.5, p=0.006) and nonischemic cardiomyopathy(r=-0.45, p=0.028). The duration of the longest diastolic electrogram was inversely correlated with the dimensions of common pathway (length: r=-0.46, p=0.001, width: r=-0.3, p=0.047) and predictive of VT termination by a single radiofrequency application (r=-0.41, p=0.023). Conclusions: Due to a wide spectrum of CV observed within the reentrant path during human VT, the dimensions of the circuit were not predictive of VT cycle length. For the first time, we demonstrate that the CV of the outer loop, rather than isthmus, is the principal determinant of the rate of VT. The size of the circuit was similar between fast versus slow VTs and unstable versus stable VTs. Long, continuous electrograms were indicative of spatially confined isthmus dimensions, confirmed by rapid termination of VT during radiofrequency delivery.

Near-optimal rotation of colour space by zebrafish cones in vivo

SummaryFor colour vision, retinal circuits must separate information about intensity and wavelength. This requires circuit-level comparison of at least two spectrally distinct photoreceptors. However, many vertebrates use four or more, and in those cases the nature and implementation of this computation remains poorly understood. Here, we establish the complete circuit architecture and function of outer retinal circuits underlying colour processing in the tetrachromatic larval zebrafish. Our findings reveal that the specific spectral tunings of the four cone types near optimally rotate the encoding of natural daylight in a principal component analysis (PCA)-like manner to yield one primary achromatic axis, two colour-opponent axes as well as a secondary UV-achromatic axis for prey capture. We note that fruit flies – the only other tetrachromat species where comparable circuit-level information is available - use essentially the same strategy to extract spectral information from their relatively blue-shifted terrestrial visual world. Together, our results suggest that rotating colour space into achromatic and chromatic axes at the eye’s first synapse may be a fundamental principle of colour vision when using more than two spectrally well-separated photoreceptor types.

On the Theory and Design of Cold Resistors

The concept of using special electrical circuit design to realize “cold resistors”, i.e., an active resistor circuitry with lower effective noise temperature, was first introduced about 80 years ago. Later on, various kinds of artificial resistors were applied in different research areas, such as gravitational wave detection, photo-amplifiers and quartz oscillators. Their proofs of concepts were experimentally proved. Unfortunately, the complete theory was not found even though several attempts had been published, sometimes with errors. In this paper, we describe a correct and complete circuit theoretical model of a cold resistor system. The results are confirmed by computer simulations. A design tool for this circuit is also shown.

Real-Time Controller Design Test Bench for High-Voltage Direct Current Modular Multilevel Converters

Modular multilevel converters (MMCs), with their inherent features and advantages over other conventional converters, have gained popularity and remain an ongoing topic of research. Many scholars have solved issues related to the operation, control, protection, and reliability of MMCs using simulation software and small hardware prototypes. We propose a novel approach for an MMC controller design with real-time systems. By utilizing a key benefit of LabVIEW Multisim co-simulation, an MMC control algorithm that can be deployed on a field-programmable gate array (FPGA) was developed in LabVIEW. The complete circuit was designed in Multisim, and a co-simulation was performed to drive an MMC model. The benefit of this topology is that control algorithms can be designed in a LabVIEW FPGA and tested with the Multisim co-simulation circuit to obtain simulation results. Once the controller works and provides satisfactory results, the same algorithm can be deployed in any NI (National Instruments) FPGA-based controller, like a compact remote input/output (RIO), to control real-time MMCs designed in an NI PCI eXtensions for Instrumentation (PXI) system. This method saves time and provides flexibility for effectively designing control algorithms and implementing them in an FPGA for real-time model implementation.