Pengaruh Pemberian Ekstrak Biji Tanaman Jarak Pagar (Jatropha Curcas. L) Terhadap Gambaran Histopatologi Hepar dan Kadar SGPT (Serum Glutamic Piruvic Transaminase) pada Tikus (Rattus norvergicus)

Jarak Pagar atau Jatropha curcas L merupakan tanaman famili Eurphobiaceae dengan kegunaan secara medis dan non medis, diantaranya yaitu sebagai kontrasepsi oral. Bagian Jarak Pagar yang sering dimanfaatkan adalah bagian biji. Namun, Jarak Pagar juga mengandung saponin, phorbol ester, kursin, tanin yang dapat menyebabkan keracunan organ. Penelitian ini dilakukan untuk mengetahui pengaruh ekstrak biji Jarak Pagar terhadap histopatologi hati dan kadar SGPT tikus Rattus Norvegicus, dengan menggunakan metode Rancangan Acak Lengkap. Hewan coba dibagi dalam 4 kelompok, setiap kelompok terdiri dari 5 ekor tikus betina. Kelompok tersebut yaitu kelompok kontrol tanpa perlakuan dan kelompok perlakuan per oral ekstrak biji Jarak Pagar pada dosis 10, 50, dan 100 mg/kg BB. Proses induksi dilakukan setiap hari selama 14 hari, pada hari ke-15 dilakukan nekropsi. Organ hati diambil untuk analisis histologi dan sampel darah diambil untuk mengukur kadar SGPT. Hasil penelitian menunjukkan bahwa pemberian ekstrak biji Jarak Pagar dapat meningkatkan kadar SGPT. Pemberian ekstrak biji Jarak Pagar pada dosis 100 mg/kg berat badan menginduksi kerusakan hepatosit paling parah di antara kelompok perlakuan.

Analisis Mutasi Gen Pancreatic Duodenal Homeobox 1 (PDX-1) Berdasarkan pada Kadar Glukosa Darah Kucing Domestik (Felis catus) Post Ovariohisterektomy (OH)

Ovariohisterectomy (OH) pada kucing domestik menyebabkan beberapa efek samping, diantaranya yaitu obesitas. Obesitas berhubungan sangat erat dengan penyakit diabetes. Gen glukoregulator yang mungkin akan menjadi penyebab resistensi insulin jika mengalami mutasi gen adalah gen PDX-1. Resistensi insulin tersebut akan berpengaruh sangat erat dengan glukosa darah dalam tubuh. Tujuan penelitian adalah melihat hubungan antara polimorfisme gen PDX-1 dengan kadar glukosa darah pada kucing domestik yang mengalami OH. Metode yang digunakan pada penelitian ini adalah amplifikasi invitro PCR gen PDX-1 dengan primer Forward ‘CCG AGC TGT CAA AGC TAG CG’ dan Reverse ‘GAA CCG GTA GAG GGT CTT GG’ dan dilanjutkan dengan sekuensing terhadap produk PCR. Hasil penelitian menunjukkan bahwa terdapat perbedaan mendasar pada urutan basa dalam sekuen gen PDX-1 pada c.435G>A, c.927A>T, dan c.584A>G. Perbedaan mendasar pada urutan gen PDX-1 merupakan silent mutation.

Review: Leptospirosis pada Anjing di Indonesia

Leptospirosis ialah penyakit infeksius yang diakibatkan oleh bakteri motil dari genus Leptospira. Prevalensi leptospirosis pada anjing di Indonesia berkisar 13,8% – 44% dengan varian Leptospira antara lain serovar Ichterohaemorrhagiae, Celledoni, Canicola, Pyrogenes, Cynopteri, Rachmati, Bataviae, Javanica, Grippotyphosa dan Tarrasovi. Leptospira sp. dapat ditularkan melalui kontak langsung atau melalui tanah dan air yang terkontaminasi Leptospira sp. Hewan pengerat, babi, kuda, hewan ternak, anjing dan berbagai hewan liar seperti tupai dan rusa dapat berperan sebagai karier Leptospira. Gejala klinis leptospirosis pada anjing di Indonesia berupa demam, letargi, anoreksia, muntah, ikterus, gangguan ginjal, dispnea, poliuria, urin berwarna kuning, dehidrasi dan kematian. Gold standard pengujian leptospirosis pada anjing adalah MAT, namun uji PCR dan ELISA memiliki sensitivitas yang lebih tinggi. Upaya pencegahan kejadian leptospirosis dilakukan dengan vaksinasi, penggunaan disinfeksi rutin pada lingkungan kandang dan mencegah anjing kontak langsung dengan hewan reservoir seperti tikus.

Indexs in About The Authors Adi Susanto ORCID iD Universitas Gadjah Mada Indonesia Nico Chandra Aditya Fakultas Biologi, Universitas Gadjah Mada Indonesia Sari'ah Cintami Damayanti Fakultas Biologi, Universitas Gadjah Mada Indonesia Hendry Saragih Fakultas Biologi, Universitas Gadjah Mada User You are logged in as... widi_1 My Profile Log Out Notifications View (59 new) Manage Journal Content Search Search Scope Browse By Issue By Author By Title Information For Readers For Authors For Librarians Keywords CMT IL-2 JSS babi classical swine fever conjunctivitis coryza demografi peternakan diazinon extract of dragon fruit peel fascioliasis liver liver histopathology malondialdehyde (MDA) mastitis subklinis mononuclear inflammatory cells open wound papua prevalensi sapi perah vaksinasi Pengaruh Pemberian Infused Water Buah Naga Merah (Hylocereus polyrhizus (F.A.C. Weber) Britton & Rose) Terhadap Performa Pertumbuhan Broiler

Penggunaan Antibiotics Growth Promotors (AGP) sintetis dalam pemeliharaan ayam broiler menyebabkan efek negatif jangka panjang. Penggunaan infused water buah naga merah (IWBNM) sebagai suplemen alami sangat diperlukan. Penelitian dilakukan guna mengetahui pengaruh IWBNM terhadap pertumbuhan ayam broiler. Rancangan acak lengkap menggunakan 5 kelompok dengan tiga ulangan adalah desain penelitian yang digunakan. Setiap ulangan berjumlah 20 ekor DOC. IWBNM diberikan melalui air minum. Perlakuan dilakukan hingga umur 21 hari. Perlakuan terdiri atas P0 (Kontrol) air biasa : P (Perlakuan) konsentrasi buah naga. Kelompok perlakuan yaitu 1 = konsentrasi 0,25% ; 2 = konsentrasi 0,5% ; 3 = konsentrasi 1% ; 4 = konsentrasi 2%. Data penelitian diuji dengan One Way ANOVA, dengan uji Least Significant Difference (LSD) dan Duncan sebagai uji lanjutan. Hasil pengujian, 1 ml IWBNM mengandung total flavonoid sebesar 676,19 µg. Pemberian IWBNM selama 21 hari secara umum dapat meningkatkan performa pertumbuhan ayam broiler. Pengukuran morfometri dan berat badan pada hari ke-21 merupakan indikator peningkatan performa pertumbuhan ayam broiler dengan rata-rata performa paling tinggi terdapat dalam kelompok P4 dibandingkan dengan P1, P2, P3, dan P0. Sedangkan untuk rasio organ visceral terhadap berat badan, IWBNM tidak berpengaruh signifikan baik pada organ jantung atau hepar

Haemobartonellosis in a Domestic Cat in Indonesia: a Case Study

Haemobartonellosis is a disease caused by Haemobartonella felis or Mycoplasma haemofelis, which attacks red blood cells. Ctenocephalides felis could transmit Haemobartonellosis. Further, vertical transmission from mother to offspring and blood transfusion from infected animals could transfer the disease to other animals. This report aimed to describe the diagnosis and medical treatment of haemobartonellosis in a domestic cat in Indonesia. Physical examinations and blood analysis were carried out. The cat weighed 1,4 kg with 39,3°C temperature, CRT was three seconds, mucosa looked pale, and many Ctenocephalides felis fleas were found. The clinical symptoms detected were weakness, loss of appetite, and hair loss. A blood test indicated normocytic normochromic anemia. The rod-like formation within erythrocytes in blood smear was observed. Infection with Hemobartonella felis was diagnosed based on the blood test and the presence of numerous Ctenocephalides felis. Doxycycline was administrated at 10 mg/kg BW once a day for 21 days. The cat recovered clinically after 21 days, with a good appetite, normal mucosal color, and normal temperature

Effectiveness of Snakehead Fish (Channa striata) and Turmeric (Curcuma domestica) Extract towards Wound Healing in Rats (Rattus norvegicus)

This study aimed to determine the effect of snakehead fish extracts (Channa striata) and turmeric extract (Curcuma domestica) towards incision wound healing in rats (Rattus norvegicus). Twenty-four male rats, aged 8-week-old, 200-300 grams of body weight divided into 4 groups: control (P0), treatment with povidone iodine as positive control (P1), treatment with snakehead fish extract (P2) and treatment with turmeric extract (P3). The rats were given premedication consisting of acepromazine 0.5 mg kg / BW and atropine 10 mg / kg BW. The rats were then anesthetized using ketamine 0.1 mg / kg BW. An incision was made on each rats’ back area along 2 cm with 0.05 mm depth and observed for 14 days. Observations were carried out every day with data collection at <7 days, 7-14 days, and 14 days. The One-Way ANOVA statistical analysis resulted that the wound fluid parameters and the duration of wound healing showed no significant difference between P0, P1, P2 and P3. This study concluded that the administration of povidone iodine, snakehead fish extract, and turmeric extract influenced the healing of incision wounds in rats.

Incidence Rate of Fascioliosis during Idul Adha 1440 H in Malang City

As an annual Muslim celebration, Eid al-Adha Feast is celebrated every year in Malang City. In 2019 feast, animals were sacrificed in Malang City consisting of 1202 cattle, 3572 goats and 302 sheep. Antemortem and postmortem examination were very important steps to ensure the quality of consumable animal product produced during the feast. The importance of these examination was to prevent zoonotic food-borne and transmissible disease from contaminated animal product to other livestocks nearby. Fascioliosis was listed into routine disease check due to its potential to reduce the quality of animal product, especially the liver. This study was held in 5 sub-districts in Malang City according to descriptive data collected by interview and structural questionnaire as well as visual observation. The result showed that fascioliosis happened in 26,03% of cattle, 2,93% of goats, and 1,32% of sheep being slaughtered. We also analized several risk factors influencing fascioliosis incidence rate such as body score condition and age. The fascioliosis tent to happen in medium and mature animal by age in respectively 31,62% of cattle, 54,28% of goats, and 75% of sheep. Thus, prevention and curative evaluation of fascioliosis should be continuously developed in order to provide excellent animal products.

Conjunctivitis in a Juvenile Red-Eared Slider (Trachemys scripta elegans)

Conjunctivitis is a common problem among red-eared sliders (Trachemys scripta elegans) with improper husbandry care. A 30 grams T. s. elegans was presented with complaints of anorexia, dullness, inflamed, and closed eyes. The owner informed that the turtle was kept indoors without ultraviolet B (UVB) light or sunlight exposure and the water was not changed by the owner for a week. Clinical examination showed inflammation and watery discharge was found on the left eye. Conjunctivitis was diagnosed based on history and clinical examination. Treatments consist of topical antibiotic drops, vitamin A and B-complex, and husbandry care improvement. The turtle started eating five days after treatment. Ten days after the treatment the turtle was brought back for a checkup and eye have recovered.

The Method of Total Erythrocyte and Leukocyte Counting in Raptors at Wildlife Rescue Centre (WRC) Jogja

Most of raptor species rehabilitated for conservation purpose at WRC Jogja are eagles. Annually, we perform general raptor examination involving conventional routine hematologic examination. However, low sample quality and over dense blood cell population during total erythrocyte and leukocyte counting were deemed as obstacles. This study aimed to give wider insight in how total erythrocyte and leukocyte counting was performed at WRC Jogja to overcome those obstacles. Firstly, handling and restraint were done physically adapting the Aba method. One-to-two half millilitres of blood was collected from the ulnar vein using 3-ml-syringe with 24-gauge-needle which was then preserved in an EDTA-containing tube and proceeded to subsequent method no more than 3 hours after collection. No hypovolemic shock was observed. During total erythrocyte and leukocyte counting using Neubauer-ruled counting chamber, we modified the dilution factor to be 250-fold and 50-fold respectively. This method yielded a countable cell density and can be applied in other species in similar condition.

Analysis of Slaughtering Points Distribution during Eid al-Adha 1440H in Malang City, East Java, Indonesia

Eid al-Adha is an annual Muslim celebration throughout the world indicated by sacrificing animals such as cattles, goats or sheep. This study aimed to analyse the distribution of slaughtering points, types and the number of animals being slaughtered as well as antemortem and postmortem abnormalities of the carcasses during Eid al-Adha 1440 H in Malang City. Data were collected from the geocode service location and the secondary data were obtained through questionnaire. This study listed a total of 145 slaughtering points located in Blimbing, Kedung Kandang, Klojen, Lowokwaru, and Sukun District with 3102 animals being sacrificed. The slaughtering points were taken place in the office yards, mosque yards, home yards, school yards or other public fields. As many as 75.86% of slaughtering points provided special shelters for the animals before being slaughtered. The antemortem examination resulted that animals from 21.38% of the slaughtering points suffered from conjunctivitis, lesions on the skin, broken horns, and lameness. Proportions of apparent postmortem abnormalities were measured respectively: cattle 64.83%, goat 41.38%, and sheep 4.14%. Hence, we recommend to further involve the veterinarians to monitor the animal condition during Eid al-Adha and to ensure the slaughtering processes were done properly.