DETERMINASI TOTAL FLAVONOID DAN TOTAL FENOLIK RIMPANG JERINGAU (Acorus calamus L.) DENGAN PERBEDAAN KONSENTRASI PELARUT

Rimpang jeringau merupakan salah satu kenaekaragaman hayati di Indonesia yang mengandung senyawa fenolik golongan flavanoid yang memiliki banyak khasiat untuk kesehatan. Rimpang. Penelitian ini bertujuan untuk mengetahui skrining fitokimia, kromatografi lapis tipis (KLT), determinasi total phenolic content (TPC) dan total flavanoid content (TFC) pada ekstrak etanol rimpang jeringau dengan metode perkolasi. Ekstrak rimpang jeringau mengandung senyawa alkaloid, tanin, flavanoid, saponin dan terpenoid pada skrining fitokimia dan KLT. Nilai TPC pada konsentrasi pelarut 50%, 70% dan 96% berturut- turut adalah 12,115±0,05 mg/g Gallat Acid Equivalent (GAE); 14,17±0,08 mg/g Gallat Acid Equivalent (GAE); 16,699±0,12 mg/g Gallat Acid Equivalent (GAE). Nilai TFC pada konsentrasi pelarut 50%, 70% dan 96% berturut- turut adalah 4,917±0,01 mg/g Rutin Equivalent (RE); 7,20±0,01 mg/g Rutin Equivalent (RE); 6,42±0,01 mg/g Rutin Equivalent (RE). Hasil penelitian dapat disimpulkan bahwa ekstrak rimpang jeringau memiliki potensi sebagai sumber fenolik dan flavonoid dengan nilai TPC tertinggi pada ekstrak konsentrasi pelarut 96% dan nilai TFC tertinggi pada ekstrak konsentrasi pelarut 70%.

OPTIMASI EMULGEL MINYAK ATSIRI ADAS (Foeniculum vulgare Mill.) SEBAGAI ANTI JERAWAT

Acne is a common skin problem. One factor that causes acne is the bacterium Propionibacterium acnes by infecting the skin, causing inflammation. Fennel essential oil (Foeniculum vulgare Mill.) Has potential as an antibacterial. This study aims to determine the combination effect of HPMC, propylene glycol and tween 80 which is formulated to be the optimum preparation of fennel essential oil emulgel with good stability as an anti-acne. Oleum Foeniculi are identified by GC-MS to determine the content of chemical compounds and are tested in vitro against Propionibacterium acnes to find out the minimum inhibitory concentration (MIC). Formula optimization is carried out using the factorial design method. The results showed that Foeniculum oil has content of Benzene, 1-methoxy-4- (2-propenyl) (83.64%), 1-Limonene (3.75%) and Fechone (3.58%) which have potential antibacterial properties against Propionibacterium acnes. Foeniculum oil with a concentration of 6% shows a MIC value of 1.7 cm. The concentration of fennel essential oil is formulated into an optimum emulgel preparation with a combination of 4% : 10% : 10.99%. of HPMC, propylene glycol, and tween 80

EFEK PEMBERIAN SEDUHAN SERBUK HERBA SELEDRI (Apium graviolens L.) TERHADAP KADAR ASAM URAT SERUM DARAH AYAM LEGHORN JANTAN HIPERURIKEMIA

Apium graviolens L. is a medicinal plant that is useful for treating gout. Flavonoids are components of chemical compounds found in celery because they can work as antioxidants and inhibit the action of the xanthine oxidase enzyme to form uric acid. This study aims to determine the effect of giving celery herb powder on the blood serum uric acid levels of hyperuricemia male leghorn chickens. This study used twenty-five male leghorn chickens which were randomly divided into 5 groups. All groups were made hyperuricemia by being given 100% chicken liver juice 5 mL/l kg BW once a day orally during treatment. Group 1 was given 0.5% CMC suspension solution as a negative control group. Group 2 was treated with allopurinol 14 mg/1.5 kg BW in CMC 0.5% as a positive control. Groups 3, 4, 5 received infusion of celery herb powder in the order of doses (0.6 g/1.5 kg BW), (1.2 g/1.5 kg BW), (1.8 g/1.5 kg BW). Examination of uric acid levels was carried out on days 0, 4, 8, 12. The results showed that steeping celery could reduce uric acid levels in proportion to the dose. The greater the dose given, the greater the decrease in uric acid levels. The dose of celery herb group 3 (1.8 g/1.5 kg BW) was the most effective doses because it had the same value as the positive control.

UJI KANDUNGAN PROTEIN FORMULASI KERANG/KOTENG (Pilsbryoconcha exilis) MENJADI MP-ASI DI DESA LAMASI PANTAI

Based on RISKESDAS data on the amount of stunting in children under five in Indonesia, this study conducted a test of the nutritional content of protein in local foodstuffs of the coastal Lamasi village community, namely Koteng mussels (Pilsbryoconcha exilis). This is to support the government of South Sulawesi to reduce the prevalence rate of stunting (very short and short) who experience malnutrition and malnutrition. This study only focuses on testing the protein content as the main substance and nutrition for the growth and development of toddlers, while the method used in this study is the shellfish formulation which is done manually where the raw material is boiled and dried then smashed into powder and then tested for protein content using the Kjeldahl method in the Sucofindo laboratory weighing 500 grams. The results of the protein content test of the formulation of Koteng shellfish (P.exilis) resulted in a protein content of 56.66%. As for the preparation of MP-ASI recipes with a collection of river clams worth 70 g, 4 g skim milk, 6 g rice flour, and 2 g vegetable oil, each containing protein worth 7,9% successively 6,5%; 9,6%; 2%

PENGARUH CARA PENGERINGAN SIMPLISIA TERHADAP KADAR FENOLIK DAN AKTIVITAS TABIR SURYA EKSTRAK ETANOL 70% DAUN CINCAU HIJAU (Cyclea barbata Miers.)

Extract cincau leaves green according to previous studies have the value of the antioxidant IC50 49,45±0,64 µg/mL. According to researchers the phenol formerly produced through a method of drying oven with higher than the drying with sunlight and dried use wind. The research aims to understand the influence of means drying simplisia against phenolic levels total and the activity of a natural sunscreen on extract ethanol 70 % cincau leaves green (Cyclea barbata Miers). Drying simplisia in research is divided into three which is drying with the dry wind, the sun, and an oven. Green leaves cincau simplisia extracted by using the method maceration. Testing the phenolic committed using the folin ciocalteu method. The determination is based on the SPF value Mansur. The results of the value of phenolic or on the program have managed to extract by the difference means drying simplisia dry wind, the rays of the sun and an oven respectively namely 32,7089; 46,2500 and 59,5500 mgGAE / g. The SPF value obtained from each extract with different methods of drying simplisia showed significant differences in various concentration test.