Culture Media, Animal Cells, Large Scale Production

2002 ◽  
Author(s):  
Jennie P. Mather ◽  
Alison Moore
Keyword(s):  
Large Scale ◽  
Culture Media ◽  
Scale Production ◽  
Animal Cells ◽  
Large Scale Production

scholarly journals Process of Establishment And In Vitro Development of Simaba Cedron Planch Seedlings. (Simaroubaceae)

2020 ◽  
Vol 8 (8) ◽  
pp. 748-759
Author(s):  
Marcia Santos de Freitas Lira ◽  
Simone Da Silva ◽  
Fábio Leandro Calderaro ◽  
Jandecy Cabral Leite
Keyword(s):  
Large Scale ◽  
Culture Media ◽  
Physical Space ◽  
Scale Production ◽  
Multiplication Rate ◽  
Large Scale Production ◽  
Snake Bites ◽  
Propagation Methods ◽  
Vitro Development

Simaba cedron, popularly known as "cedron", is largely used for fever and snake bites. Its seeds are used in the treatment of stomach problems and liver infections. The fruits are used for the treatment of pain and malaria while its bark is an antispasmodic. Simaba cedron is generally propagated through seeds, but with limited success, as the low viability of same restricts its propagation. In view of such difficulty, it becomes necessary the study for adequate conditions for the large scale production of these seedlings. Being it known that in several species, the use of micropropagation has made it possible to obtain a large amount of disease-free and more homogeneous seedlings, in reduced time and physical space, in comparison with conventional propagation methods, the objective of this work was to analyze the effect of two culture media on the production of aseptic parent plants as a first step in the development of a micropropagation protocol for Simaba cedron. The seeds were collected from a matrix plant located in the Amazon Biotechnology Center (CBA), in Manaus/AM. The experiment was installed at the Vegetable Tissue Culture Laboratory, where the  explants were desinfected and grown in culture medium  according to Murashige & Skoog (MS) and in Wood Plant Medium (WPM), during 60 days. The disinfestation rate obtained was 75% and, of the disinfested seeds, 100% germinated. The cultivation medium that was more favorable to the cultivation of simaba was the MS, where the multiplication rate was of 8.0: 1, whose seedlings reached, in average, 4.8 cm and 75% of rooting.

scholarly journals Cell-Based Fish: A Novel Approach to Seafood Production and an Opportunity for Cellular Agriculture

2019 ◽  
Author(s):  
Natalie Rubio ◽  
Isha Datar ◽  
David Stachura ◽  
Kate Krueger
Keyword(s):  
Cell Culture ◽  
Cell Cultures ◽  
Closed System ◽  
Biomedical Engineering ◽  
Large Scale ◽  
Fish Cell ◽  
Scale Production ◽  
Animal Cells ◽  
Large Scale Production ◽  
Physiological Properties

Cellular agriculture is defined as the production of agricultural products from cell cultures rather than from whole plants or animals. With growing interest in cellular agriculture as a means to address the public health, environmental, and animal welfare challenges of animal agriculture, the concept of producing seafood from fish cell- and tissue-cultures is emerging as a means to address similar challenges with industrial aquaculture systems and marine capture. Cell-based seafood - as opposed to animal-based seafood - can combine developments in biomedical engineering with modern aquaculture techniques. Biomedical engineering developments such as closed-system bioreactor production of land animal cells create a basis for large scale production of marine animal cells. Aquaculture techniques such as genetic modification and closed system aquaculture have achieved marked gains in production that can pave the way for innovations in cell-based seafood production. Here, we present the current state of innovation relevant to the development of cell-based seafood across multiple species as well as specific opportunities and challenges that exist for advancing this science. The authors find that the physiological properties of fish cell- and tissue- culture may be uniquely suited to cultivation in vitro. These physiological properties, including hypoxia tolerance, high buffering capacity, and low-temperature growth conditions, make marine cell culture an attractive opportunity for scale production of cell-based seafood; perhaps even more so than mammalian and avian cell cultures for cell-based meats. This, coupled with the unique capabilities of crustacean tissue-friendly scaffolding such as chitosan, a common seafood waste product and mushroom derivative, presents great promise for cell-based seafood production via bioreactor cultivation. To become fully realized, cell-based seafood research will require more understanding of fish muscle culture and cultivation; more investigation into serum-free media formulations optimized for fish cell culture; and bioreactor designs tuned to the needs of fish cells for large scale production.

scholarly journals Culture Media for Large-scale Production of Micrococcin

1952 ◽  
Vol 6 (1-2) ◽  
pp. 41-46 ◽  
Author(s):  
B. K. KELLY ◽  
G. A. MILLER ◽  
C. W. HALE
Keyword(s):  
Large Scale ◽  
Culture Media ◽  
Scale Production ◽  
Large Scale Production

scholarly journals Biotechnology of biomass production in vitro of fungi isolated from Pinus

2020 ◽  
Vol 9 (10) ◽  
pp. e7809109080
Author(s):  
Paula Francislaine Moura ◽  
Celso Garcia Auer ◽  
Katlin Suellen Rech ◽  
Camila Freitas de Oliveira ◽  
Cristiane da Silva Paula de Oliveira ◽  
...  
Keyword(s):  
Biomass Production ◽  
Large Scale ◽  
Culture Media ◽  
In Vitro Cultivation ◽  
Malt Extract ◽  
Scale Production ◽  
Potato Dextrose Broth ◽  
Mechanical Stirring ◽  
Large Scale Production

Fungi are organisms capable of synthesizing metabolites of industrial interest and the standardization of biomass production for the extraction of these compounds has biotechnological applications. The objective of this work was to optimize the in vitro cultivation process for fungi isolated from Pinus sp., standardizing the best conditions for the production of biomass, contributing to its large scale production. Therefore, the conditions of in vitro cultivation of the fungi Botrytis cinerea, Rhizoctonia sp. and Suillus sp., were evaluated based on the maximum production of dry biomass (PBS), varying temperature, medium and cultivation time. The fungi were grown in glass flasks with liquid culture media, in a BOD chamber, without mechanical stirring. Potato-dextrose broth - PD broth (PD), Czapek - CZ broth (CZ) and Malt Extract - EM broth (EM) were evaluated at temperatures ranging from 8 to 32 ºC and incubation times from 7 to 35 days. PD broth showed better results for fungi B.cinerea and Rhizoctonia sp., when compared to CZ and EM broths, in PBS, while Suillus sp. showed better development in EM broth. The best growth temperature based on PBS was 12 ºC and 16 ºC, with 28 and 35 days of cultivation.

scholarly journals Cell-Based Fish: A Novel Approach to Seafood Production and an Opportunity for Cellular Agriculture

2018 ◽  
Author(s):  
Natalie Rubio ◽  
Isha Datar ◽  
David Stachura ◽  
Kate Krueger
Keyword(s):  
Cell Culture ◽  
Cell Cultures ◽  
Closed System ◽  
Biomedical Engineering ◽  
Large Scale ◽  
Fish Cell ◽  
Scale Production ◽  
Animal Cells ◽  
Large Scale Production ◽  
Physiological Properties

Cellular agriculture is defined as the production of agricultural products from cell cultures rather than from whole plants or animals. With growing interest in cellular agriculture as a means to address the public health, environmental, and animal welfare challenges of animal agriculture, the concept of producing seafood from fish cell- and tissue-cultures is emerging as a means to address similar challenges with industrial aquaculture systems and marine capture. Cell-based seafood - as opposed to animal-based seafood - can combine developments in biomedical engineering with modern aquaculture techniques. Biomedical engineering developments such as closed-system bioreactor production of land animal cells create a basis for large scale production of marine animal cells. Aquaculture techniques such as genetic modification and closed system aquaculture have achieved marked gains in production that can pave the way for innovations in cell-based seafood production. Here, we present the current state of innovation relevant to the development of cell-based seafood across multiple species as well as specific opportunities and challenges that exist for advancing this science. The authors find that the physiological properties of fish cell- and tissue- culture may be uniquely suited to cultivation in vitro. These physiological properties, including hypoxia tolerance, high buffering capacity, and low-temperature growth conditions, make marine cell culture an attractive opportunity for scale production of cell-based seafood; perhaps even more so than mammalian and avian cell cultures for cell-based meats. This, coupled with the unique capabilities of crustacean tissue-friendly scaffolding such as chitosan, a common seafood waste product and mushroom derivative, presents great promise for cell-based seafood production via bioreactor cultivation. To become fully realized, cell-based seafood research will require more understanding of fish muscle culture and cultivation; more investigation into serum-free media formulations optimized for fish cell culture; and bioreactor designs tuned to the needs of fish cells for large scale production.

I. S. A. Baud. Forms of Production and Women's Labour: Gender Aspects of Industrialisation in India and Mexico. New Delhi: Sage Publications. 1992. 321 pp.Hardbound. Price: Indian Rs 295.00.

1993 ◽  
Vol 32 (1) ◽  
pp. 129-131
Author(s):  
Naureen Talha
Keyword(s):  
Large Scale ◽  
Indian Society ◽  
New Delhi ◽  
Scale Production ◽  
Female Labour ◽  
Self Employment ◽  
Commodity Production ◽  
Large Scale Production ◽  
Mexican Society ◽  
Small Production

The literature on female labour in Third World countries has become quite extensive. India, being comparatively more advanced industrially, and in view of its size and population, presents a pictures of multiplicity of problems which face the female labour market. However, the author has also included Mexico in this analytical study. It is interesting to see the characteristics of developing industrialisation in two different societies: the Indian society, which is conservative, and the Mexican society, which is progressive. In the first chapter of the book, the author explains that he is not concerned with the process of industrialisation and female labour employed at different levels of work, but that he is interested in forms of production and women's employment in large-scale production, petty commodity production, marginal small production, and self-employment in the informal sector. It is only by analysis of these forms that the picture of females having a lower status is understood in its social and political setting.

An Efficient and Improved Process for the Synthesis of Itopride Hydrochloride and Trimethobenzamide Hydrochloride

2018 ◽  
Vol 15 (4) ◽  
pp. 572-575 ◽  
Author(s):  
Ponnusamy Kannan ◽  
Samuel I.D. Presley ◽  
Pallikondaperumal Shanmugasundaram ◽  
Nagapillai Prakash ◽  
Deivanayagam Easwaramoorthy
Keyword(s):  
Large Scale ◽  
Hydroxylamine Hydrochloride ◽  
Scale Production ◽  
One Pot ◽  
Hydrochloride Salt ◽  
Environmental Friendly ◽  
Large Scale Production ◽  
Non Ulcer Dyspepsia ◽  
Itopride Hydrochloride ◽  
Yield And Purity

Aim and Objective: Itopride is a prokinetic agent used for treating conditions like non-ulcer dyspepsia. Itopride is administered as its hydrochloride salt. Trimethobenzamide is used for treating nausea and vomiting and administered as its hydrochloride salt. The aim is to develop a novel and environmental friendly method for large-scale production of itopride and trimethobenzamide. Materials and Methods: Itopride and trimethobenzamide can be prepared from a common intermediate 4- (dimethylaminoethoxy) benzyl amine. The intermediate is prepared from one pot synthesis using Phyrdroxybenzaldehye and zinc dust and further reaction of the intermediate with substituted methoxy benzoic acid along with boric acid and PEG gives itopride and trimethobenzamide. Results: The intermediate 4-(dimethylaminoethoxy) benzylamine is prepared by treating p-hydroxybenzaldehyde and 2-dimethylaminoethyl chloride. The aldehyde formed is treated with hydroxylamine hydrochloride. The intermediate is confirmed by NMR and the purity is analysed by HPLC. Conclusion: Both itopride and trimethobenzamide were successfully synthesized by this method. The developed method is environmental friendly, economical for large-scale production with good yield and purity.

scholarly journals Cyanobacteria—From the Oceans to the Potential Biotechnological and Biomedical Applications

Marine Drugs ◽  
2021 ◽  
Vol 19 (5) ◽  
pp. 241
Author(s):  
Shaden A. M. Khalifa ◽  
Eslam S. Shedid ◽  
Essa M. Saied ◽  
Amir Reza Jassbi ◽  
Fatemeh H. Jamebozorgi ◽  
...  
Keyword(s):  
Bioactive Compounds ◽  
Biomedical Applications ◽  
Large Scale ◽  
Biological Activities ◽  
Scale Production ◽  
Pharmacological Activities ◽  
Large Scale Production ◽  
Marine Products ◽  
Hiv 1 ◽  
Successful Phase

Cyanobacteria are photosynthetic prokaryotic organisms which represent a significant source of novel, bioactive, secondary metabolites, and they are also considered an abundant source of bioactive compounds/drugs, such as dolastatin, cryptophycin 1, curacin toyocamycin, phytoalexin, cyanovirin-N and phycocyanin. Some of these compounds have displayed promising results in successful Phase I, II, III and IV clinical trials. Additionally, the cyanobacterial compounds applied to medical research have demonstrated an exciting future with great potential to be developed into new medicines. Most of these compounds have exhibited strong pharmacological activities, including neurotoxicity, cytotoxicity and antiviral activity against HCMV, HSV-1, HHV-6 and HIV-1, so these metabolites could be promising candidates for COVID-19 treatment. Therefore, the effective large-scale production of natural marine products through synthesis is important for resolving the existing issues associated with chemical isolation, including small yields, and may be necessary to better investigate their biological activities. Herein, we highlight the total synthesized and stereochemical determinations of the cyanobacterial bioactive compounds. Furthermore, this review primarily focuses on the biotechnological applications of cyanobacteria, including applications as cosmetics, food supplements, and the nanobiotechnological applications of cyanobacterial bioactive compounds in potential medicinal applications for various human diseases are discussed.

scholarly journals A Review on Additive Manufacturing Possibilities for Electrical Machines

Energies ◽  
2021 ◽  
Vol 14 (7) ◽  
pp. 1940
Author(s):  
Muhammad Usman Naseer ◽  
Ants Kallaste ◽  
Bilal Asad ◽  
Toomas Vaimann ◽  
Anton Rassõlkin
Keyword(s):  
Additive Manufacturing ◽  
Large Scale ◽  
Three Dimensional ◽  
Electrical Machines ◽  
Electromagnetic Properties ◽  
Scale Production ◽  
Performance Parameters ◽  
Large Scale Production ◽  
One Step ◽  
Manufacturing Techniques

This paper presents current research trends and prospects of utilizing additive manufacturing (AM) techniques to manufacture electrical machines. Modern-day machine applications require extraordinary performance parameters such as high power-density, integrated functionalities, improved thermal, mechanical & electromagnetic properties. AM offers a higher degree of design flexibility to achieve these performance parameters, which is impossible to realize through conventional manufacturing techniques. AM has a lot to offer in every aspect of machine fabrication, such that from size/weight reduction to the realization of complex geometric designs. However, some practical limitations of existing AM techniques restrict their utilization in large scale production industry. The introduction of three-dimensional asymmetry in machine design is an aspect that can be exploited most with the prevalent level of research in AM. In order to take one step further towards the enablement of large-scale production of AM-built electrical machines, this paper also discusses some machine types which can best utilize existing developments in the field of AM.

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