Applications of Molecular Biology in Plant Breeding: The Detection of Genetic Variation and Viral Pathogens

Author(s):  
R. B. Flavell ◽  
R. J. Kemble ◽  
R. E. Gunn ◽  
A. Abbott ◽  
D. Baulcombe
1991 ◽  
Vol 10 (1) ◽  
pp. 18-25
Author(s):  
D. I. Ferreira

Conventional plant breeding has made a significant impact on the increase in crop production during the last half century. Several shortcomings however, opened up the opportunities for the application of biotechnology in plant breeding. The vari­ous approaches in the field of cell biology (tissue culture) and molecular biology (recombinant DNA technology) are dis­cussed and the application thereof is advocated in a global approach to plant breeding.


1993 ◽  
Vol 14 (2) ◽  
Author(s):  
R.A. Richards ◽  
C. L�pez-Casta�eda ◽  
H. Gomez-Macpherson ◽  
A.G. Condon

2003 ◽  
Vol 84 (12) ◽  
pp. 3191-3201 ◽  
Author(s):  
Mauro Costa-Mattioli ◽  
Anna Di Napoli ◽  
Virginie Ferré ◽  
Sylviane Billaudel ◽  
Raul Perez-Bercoff ◽  
...  

Knowledge of the molecular biology of hepatitis A virus (HAV) has increased exponentially since its identification. HAV exploits all known mechanisms of genetic variation to ensure survival, including mutation and genetic recombination. HAV has been characterized by the emergence of different genotypes, three human antigenic variants and only one major serotype. This paper reviews the genetic variability and molecular epidemiology of HAV. Its evolutionary mechanisms are described with particular emphasis on genetic recombination and HAV mutation rate. Genotypic classification methods are also discussed.


1970 ◽  
Vol 75 (1) ◽  
pp. 159-167 ◽  
Author(s):  
A. J. Thomson ◽  
H. H. Rogers

SUMMARYFive selected clones of Lolium perenne were selfed and the ten possible diallel crosses, without reciprocals, made. The progenies were grown in a split-plot design in which whole plots comprised the four nitrogen rates (0, 225, 450 and 675 kg/ha of N) and sub-plots the fifteen progenies, square planted at 0·25 m.The percentage of the applied nitrogen recovered, and the utilization of the nitrogen by the plant, were measured. Data were collected from four harvests in each of two consecutive years. The diallel was analysed by Griffing's (1956) Method II, Model II.There were significant effects for nitrogen rates, progenies and their interaction for both ‘recovery’ and ‘utilization’, but years were significantly different for ‘recovery’ only. The effect of applied nitrogen was mainly linear and it was the linear effect of applied nitrogen which interacted most with the progeny effects.Significant g.c.a. and s.c.a. effects were found but they varied between nitrogen rates and years. For ‘utilization’ the interaction nitrogen rates x g.c.a. was significant on two year means but there was no significance for the nitrogen interaction with s.c.a. The g.c.a. values obtained for both parameters did reveal some trends between the five parents.The presence of significant additive and non-additive genetic variation is discussed in relation to a plant breeding programme.


Author(s):  
Nathaniel Schleif ◽  
Shawn M. Kaeppler ◽  
Heidi F. Kaeppler

AbstractPlant breeding relies on the presence of genetic variation, which is generated by a random process of mutagenesis that acts on existing gene pools. This variation is then recombined into new forms at frequencies impacted by the local euchromatin and heterochromatin environment. The result is a genetic lottery where plant breeders face increasingly low odds of generating a “winning” plant genotype. Genome editing tools enable targeted manipulation of the genome, providing a means to increase genetic variation and enhancing the chances for plant breeding success. Editing can be applied in a targeted way, where known genetic variation that improves performance can be directly brought into lines of interest through either deletion or insertion. This empowers approaches that are traditionally difficult such as novel domestication and introgression of wild accessions into a germplasm pool. Furthermore, broader editing-mediated approaches such as recombination enhancement and targeted random mutagenesis bring novel ways of variation creation to the plant breeding toolbox. Continued development and application of plant genome editing tools will be needed to aid in meeting critical global crop improvement needs.


Author(s):  
Indah Anita Sari ◽  
Bayu Setyawan ◽  
Marcelinus A.S. Adhiwibawa ◽  
Agung Wahyu Susilo

A problem encountered in plant breeding process to determine bean color quality of fine-flavor cocoa is a long selection period. Preliminary results indicatedthat the fine-flavor cocoa has a low color reflectance than bulk cocoa. The objectiveof this study is to find more applicable and easier method to do the early detectionof fine-flavor cocoa on the breeding population. Detection of the leaf color haracteristics was done by chromatographic and spectrophotometry analysis. hromatographic analysis was carried out in Kaliwining Experimental Station, IndonesianCoffee and Cocoa Research Institute using complete random design consistedof two types of cocoa; fine-flavor (10 clones) and bulk cocoa (10 clones). pectrophotometric analysis was conducted at Ma Chung University, Malang, East Java.Four clones of bulk cocoa and six clones of fine flavor cocoa were used in thisanalysis. The results of the study showed that the flush color characteristics of thefine-flavor cocoa were different from the bulk cocoa characteristics as shown bythe differences in the value of L*, b* and anthocyanin content. Flush characteristic s ofthe fine-flavor cocoa had brighter color, more toward green with higher yellowlevel, while the bulk cocoa had darker flush, red and lower yellow level. Thecolor parameter of L* and b* had a higher genetic variation compared with theenvironment variation. However, the parameter a* had a higher environmental variation compared with genetic variation. Grouping of fine-flavor cocoa and bulk cocoacould be done through the detection of flush anthocyanin content, when tendencyof flush anthocyanin content on fine-flavor cocoa was lower than bulk cocoa.The detection of flush color particularly L* and b* parameters through hromatographic analysis and anthocyanin content through spectrophotometric analysismay be used as a criteria for selection the fine flavor cocoa on seedling phase.


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