Estimation of Biomass Concentration

2014 ◽  
pp. 65-102
Author(s):  
Sihem Tebbani ◽  
Filipa Lopes ◽  
Rayen Filali ◽  
Didier Dumur ◽  
Dominique Pareau
2000 ◽  
Vol 41 (4-5) ◽  
pp. 393-400 ◽  
Author(s):  
J.M. Garrido-Fernandez ◽  
R. Méndez ◽  
J.M. Lema ◽  
V. Lazarova

Three Circulating Floating Bed Reactors (CFBR) R1, R2 and R3 with 20% v/v of a plastic carrier with different size distribution were operated to study the effect of the particles size of the carrier on biomass accumulation and nitrification performance. Operating conditions were similar in the three systems: ammonia concentrations around 50 mg-N–NH4+/ L, ammonia loading rates up to 1.2 kg N–NH4+/m3·d and temperatures between 14 and 27°C. Accumulation of nitrite was observed until day 65th. This w as result both of the inhibition of nitrite oxidation by free ammonia until day 20th and the insignificant accumulation of a biomass with low nitrite oxidising capacity between days 20 and 65th. Ammonia conversion rate and removal efficiency were higher in the reactor with lower particle size, R3 (nitrification rate of 1.1 kg N–NH4+/m3·d and ammonia removal of 97% at 16°C), than in R2 or R1 (nitrification rate of 1.0 kg N–NH4+/m3·d and ammonia removal of 90% at 16°C). The better efficiency in R3 was obtained as a result of the higher specific surface of the biofilm developed. Biomass activity was similar in the three reactors (2.2 and 1.12 g N/g protein · d at 30 and 15°C, respectively). Both the biomass evolution with time and biomass retention in the systems was practically not influenced by the size of particle. Biomass concentration of 1.2 g protein/L was retained in the carrier and up to 20% of the newly produced biomass was retained in the CFBRs.


1986 ◽  
Vol 18 (7-8) ◽  
pp. 239-248 ◽  
Author(s):  
Sung Ryong Ha ◽  
Dwang Ho Lee ◽  
Sang Eun Lee

Laboratory scale experiments were conducted to develop a mathematical model for the anaerobic digestion of a mixture of night soil and septic tank sludge. The optimum mixing ratio by volume between night soil and septic tank sludge was found to be 7:3. Due to the high solids content in the influent waste, mixed-liquor volatile suspended solids (MLVSS) was not considered to be a proper parameter for biomass concentration, therefore, the active biomass concentration was estimated based on deoxyribonucleic acid (DNA) concentration in the reactor. The weight ratio between acidogenic bacteria and methanogenic bacteria in the mixed culture of a well-operated anaerobic digester was approximately 3:2. The proposed model indicates that the amount of volatile acid produced and the gas production rate can be expressed as a function of hydraulic residence time (HRT). The kinetic constants of the two phases of the anaerobic digestion process were determined, and a computer was used to simulate results using the proposed model for the various operating parameters, such as BOD5 and volatile acid concentrations in effluent, biomass concentrations and gas production rates. These were consistent with the experimental data.


2021 ◽  
Vol 11 (7) ◽  
Author(s):  
Nur Syamimi Zaidi ◽  
Johan Sohaili ◽  
Khalida Muda ◽  
Mika Sillanpää ◽  
Norelyza Hussein

AbstractLow condition of dissolved oxygen (DO) is commonly associated with sludge bulking problem that was able to disrupt the efficiency of wastewater treatment performances. Relatively, very little attention was paid to the possibility of applying magnetic field in controlling the bulking problem. Hence, this study aims to investigate the performance of magnetic field on biomass properties and its effect on biodegradation under low condition of DO. Two continuous laboratory-scale sequencing batch reactors—Reactor A (SBRA) and Reactor B (SBRB)—were setup. SBRA was equipped with the magnetic device to exhibit magnetic field of 88 mT, while SBRB acted as a control system. The results showed that the biomass concentration in SBRA was higher compared to SBRB. High biomass concentration in SBRA resulted to better settleability with mean SVI of less than 30 mL/g. SBRA also showed consistently high removal performances of organic and inorganic contents compared to SBRB. These observations confirmed that the magnetic field was able to enhance the biomass properties, which further enhance the biodegradation ability of the aerobic bacteria under low DO condition. This also indicates that under the sludge bulking circumstances, the use of magnetic field stands a great chance in maintaining high biodegradation of the treatment system.


2021 ◽  
Vol 640 (7) ◽  
pp. 072013
Author(s):  
I A Avtsinov ◽  
A N Gavrilov ◽  
N V Sukhanova ◽  
Yu E Kozhevnikov ◽  
S A Nickel ◽  
...  

Author(s):  
Władysława Wojciechowska ◽  
Tomasz Lenard

AbstractThe research was carried out in a mesotrophic and dimictic lake during winters with ice cover. In the last forty years, the development of phytoplankton was analyzed in five extreme winter seasons. The studies of phytoplankton characteristics in the water column took into account values of biomass, concentration of chlorophyll-a and species composition, including dominant species. Differences in the vertical distribution of flagellate and non-flagellate species belonging to cyanobacteria and algae were analyzed in the gradient of light and thermal conditions. The phytoplankton biomass was low and vertically differentiated, with the lowest values at the deeper part of the water column. Flagellate species from the group of Cryptophyceae, Chrysophyceae and Dinophyceae were most abundant. Species biodiversity was low but every winter the dominant species represented different taxonomic groups. In some periods, larger non-motile phytoplankton species from green or blue-green algae dominated. The research proved that the development of phytoplankton under the ice cover was limited mainly by light and, to a lesser extent, by temperature.


Author(s):  
R. Ramjug-Ballgobin ◽  
K. Busawon ◽  
R. T. F. Ah King ◽  
H. C. S. Rughooputh

1993 ◽  
Vol 28 (7) ◽  
pp. 139-142 ◽  
Author(s):  
J. Chang ◽  
P. Chudoba ◽  
B. Capdeville

Maintenance energy plays an important role both in basic kinetic studies and in process development. Numerous studies have been devoted to the maintenance concept in various microbial fields but very few in biological wastewater treatment. Using a fermenter coupled to a mass spectrometer, we investigated the influence of the ratio So/Xo (ratio between initial substrate concentration and initial biomass concentration) on the observed sludge growth yield of an oxic-settling anaerobic (OSA) system. By measuring the substrate removed, the oxygen consumed and the carbon dioxide produced, we were able to estimate the substrate fraction used for maintenance purposes. The results indicate that at a high So/Xo ratio, a greater proportion of the substrate is devoted to maintenance thus significantly decreasing the observed growth yield. These findings are of particular importance in view of the cost associated with the disposal of excess sludge in aerobic wastewater treatment processes.


2006 ◽  
Vol 60 (6) ◽  
Author(s):  
M. Juraščík ◽  
M. Hucík ◽  
I. Sikula ◽  
J. Annus ◽  
J. Markoš

AbstractThe effect of the biomass presence on the overall circulation velocity, the linear velocities both in the riser and the downcomer and the overall gas hold-up was studied in a three-phase internal loop airlift reactor (ILALR). The measured data were compared with those obtained using a two-phase system (air—water). All experiments were carried out in a 40 dm3 ILALR at six different biomass concentrations (ranging from 0 g dm−3 to 7.5 g dm−3), at a temperature of 30°C, under atmospheric pressure. Air and water were used as the gas and liquid model media, respectively. Pellets of Aspergillus niger produced during the fermentation of glucose to gluconic acid in the ILALR were considered solid phase. In addition, liquid velocities were measured during the fermentation of glucose to gluconic acid using Aspergillus niger. All measurements were performed in a bubble circulation regime. At given experimental conditions the effect of the biomass on the circulation velocities in the ILALR was negligible. However, increasing of the biomass concentration led to lower values of the total gas hold-up.


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