Tissue engineering involves the use of living cells and cell scaffolds to develop biological substitutes for tissue replacements, it is one of promising ways for rehabilitation and reconstruction functional tissue and organs. In order to engineer substitutes for tissue replacements, cell scaffolds with specific shape and pore structure are required. A novel “elastic porogen/pressure filtration technique” was put forward and studied firstly in this paper to overcome the disadvantages of the existed techniques for cell scaffold fabrication. The properties of elastic porogen (deformation ratio, water solubility, appearance and dimension) and pore structure of scaffolds were studied, respectively. The experimental results demonstrated that the scaffolds with well defined pore structure can be formed through this novel technique, and the pore shape and sizes as well as size of openings between pores could be manual controlled conveniently. The pore structure and morphology of scaffolds were satisfied to the requirements of tissue engineering, which suggested that elastic porogen/pressure filtration technique was an ideal cell scaffold forming technique.