Microbial Resistance: Silver Covalently Bound to Cyanographene Overcomes Bacterial Resistance to Silver Nanoparticles and Antibiotics (Adv. Sci. 12/2021)

In this research, a thermo-sensitive copolymer PNIPAAm-co-MHq was used to successfully synthesize different nano sizes silver nanoparticles in the ranges between 1.5 to 4 nm with uniform dispersion. The polymeric assisted synthesized Ag nanoparticles (AgNPs@PM) exhibited reasonable solution stability and thermalresponsive behaviour. In specific, AgNPs@PM3 (1.59 nm) displayed improved bacterial resistance against clinically approved anti-biotic resistant bacterial pathigens with very low MIC value (4.05 μg/mL). Subsequently, the thermal responsive polymeric molecular structure on AgNPs synthesis has been established that significant temperature dependened anti-bacterial efficiency. It was also observed that the nonparticipants size, temperature responses and proportion of thermosensitive copolymer also influenced the antibacterial efficacy of AgNPs@PM. Resulting thermal sensitive polymer nanocomposite can be extremely beneficial for wound healing treatment after femoral fracture surgery.

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Antibacterial Effect of Silver Nanoparticles on Klebsiella spp

Healthcare Review ◽

10.47285/hr.v1i2.54 ◽

2020 ◽

Vol 1 (2) ◽

pp. 8-15

Author(s):

Gislanne Stéphanne Estevam da Silva ◽

Rivaldo Leon Bezerra Cabral ◽

Nathalie de Sena Pereira ◽

José Heriberto Oliveira do Nascimento ◽

Dany G kramer

Keyword(s):

Silver Nanoparticles ◽

Bacterial Resistance ◽

Brain Heart Infusion ◽

Trisodium Citrate ◽

Antimicrobial Activities ◽

Positive Control ◽

Negative Control ◽

In Vitro Tests ◽

Klebsiella Spp

Silver nanoparticles (AgNP) can be incorporated into medical devices, such as tissues, to circumvent bacterial resistance such as Klebsiella spp, which can lead to skin and mucosal infections. Thus, the aim of the present study was to synthesize silver nanoparticles for later incorporation into cotton fabrics and in vitro tests against Klebsiella spp. The AgNP colloidal solution was synthesized (AgNO3 - 0.1 mM, 100 mM trisodium citrate, polyvinylpyrrolidone - 0.24 g, H2OH2) and then impregnated into the cotton fabric pretreated with poly diallyl dimethylammonium chloride (PDDA) of 100/500 tissue, shaken for 30 minutes). The material produced was analyzed by the FTIR; DLS and reflectance spectroscopy. The tests of the antimicrobial activities were by the microdilution technique against Klebsiella spp, in tubes containing Brain Heart Infusion (BHI), with the solution of silver (1); Tissue containing AgNP - 4 mm (2); Negative control (3) and positive control - ceftriaxone (4). Regarding MIC, the inhibitory activity occurred of the dilutions between 1/2 and 1/16. The AgNP particles had an average size of 24.75 nm. As synthesized AgNPs demonstrate the excellent antimicrobial activity against Klebsiella spp, with special emphasis on applications in nanotechnology and nanomedicine, targeting multiresistant antibiotic bacteria.

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Systems-level analysis of Escherichia coli response to silver nanoparticles: The roles of anaerobic respiration in microbial resistance

Biochemical and Biophysical Research Communications ◽

10.1016/j.bbrc.2012.06.134 ◽

2012 ◽

Vol 424 (4) ◽

pp. 657-662 ◽

Cited By ~ 19

Author(s):

Huamao Du ◽

Tat-Ming Lo ◽

Johnner Sitompul ◽

Matthew Wook Chang

Keyword(s):

Escherichia Coli ◽

Silver Nanoparticles ◽

Anaerobic Respiration ◽

Microbial Resistance ◽

Level Analysis

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Characterization of Silver Nanoparticles Obtained by a Green Route and Their Evaluation in the Bacterium of Pseudomonas aeruginosa

Crystals ◽

10.3390/cryst10050395 ◽

2020 ◽

Vol 10 (5) ◽

pp. 395 ◽

Cited By ~ 1

Author(s):

Juan Carlos Martínez Espinosa ◽

Raúl Carrera Cerritos ◽

Maria Antonieta Ramírez Morales ◽

Karla Paola Sánchez Guerrero ◽

Rocio Alejandra Silva Contreras ◽

...

Keyword(s):

Electron Microscopy ◽

Transmission Electron Microscopy ◽

Pseudomonas Aeruginosa ◽

Silver Nanoparticles ◽

Photoelectron Spectroscopy ◽

Bacterial Resistance ◽

Antimicrobial Effect ◽

X Ray ◽

Transmission Electron ◽

Green Route

Metal nanoparticles are widely used in different areas such as biotechnology and biomedicine, for example in drug delivery, imaging and control of bacterial growth. The antimicrobial effect of silver has been identified as an alternative approach to the increasing bacterial resistance to antibiotics. Silver nanoparticles were synthesized by the green route using the Geranium extract as a reducing agent. The characterization was carried out by the techniques of UV-Vis spectrophotometry, transmission electron microscopy (TEM), high-resolution transmission electron microscopy (HRTEM), X-ray emitted photoelectron spectroscopy (XPS) and X-ray diffraction. Nanoparticle diameters between 15 and 50 nm were obtained and the interplanar spaces calculated from the electron diffraction pattern corresponding to a mixture of silver with 4H and FCC structures. To determine the minimum inhibitory concentration of silver nanoparticles (AgNPs) on the Pseudomonas aeruginosa bacteria (ATCC-27853), different concentrations of colloidal solution 0.36, 0.18, 0.09 and 0.05 μg/mL were evaluated as a function of the incubation time, measuring the inhibition halo and colony forming unit (CFU) during 0, 2 and 4 h of incubation. The minimum inhibitory AgNPs concentration (MIC) is 0.36 μg/mL at 0 h while the concentration of 0.18 μg/mL presents a total inhibition of the bacterium after 2 h. For the rest of the dilutions, gradual inhibitions as a function of time were observed. We evaluate the antibacterial effect of silver nanoparticles obtained by a green methodology in Pseudomonas aeruginosa bacteria. Finally, the colloidal nanoparticle solution can be an antibacterial alternative for different biomedical approaches.

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Bacterial resistance to silver nanoparticles and how to overcome it

Nature Nanotechnology ◽

10.1038/s41565-017-0013-y ◽

2017 ◽

Vol 13 (1) ◽

pp. 65-71 ◽

Cited By ~ 239

Author(s):

Aleš Panáček ◽

Libor Kvítek ◽

Monika Smékalová ◽

Renata Večeřová ◽

Milan Kolář ◽

...

Keyword(s):

Silver Nanoparticles ◽

Bacterial Resistance

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Bacterial virulence factors: a target for heterocyclic compounds to combat bacterial resistance

RSC Advances ◽

10.1039/d1ra06238g ◽

2021 ◽

Vol 11 (58) ◽

pp. 36459-36482

Author(s):

Rehab H. Abd El-Aleam ◽

Riham F. George ◽

Hanan H. Georgey ◽

Hamdy M. Abdel-Rahman

Keyword(s):

Virulence Factors ◽

Heterocyclic Compounds ◽

Bacterial Resistance ◽

Bacterial Virulence ◽

Microbial Resistance

This review presents an overview of different heterocyclic compounds that act as anti-virulence agents in order to overcome microbial resistance.

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Antimicrobial applications of nanoliposome encapsulated silver nanoparticles: a potential strategy to overcome bacterial resistance

Current Nanoscience ◽

10.2174/1573413716999200712184148 ◽

2020 ◽

Vol 16 ◽

Author(s):

M.R. Mozafari ◽

Sarabanou Torkaman ◽

Fatemeh Mahsa Karamouzian ◽

Babak Rasti ◽

Bikash Baral

Keyword(s):

Silver Nanoparticles ◽

Bacterial Infections ◽

Bacterial Resistance ◽

Water Soluble ◽

Severe Illness ◽

Efficient System ◽

Antimicrobial Substances ◽

Potential Strategy

: Bacterial infections result in hundreds of million cases of severe illness annually worldwide. Rapidly increasing drug resistance of pathogens further aggravates this threat to human health and warrants the search for effective broadspectrum antibacterial agents. Silver metal has a long history of application in human medicine and healthcare. In ancient times, silver was employed as a disinfectant for water purification and storage while it is still being used as an antimicrobial ingredient in some nanotechnology-based products. Encapsulation of antimicrobial substances such as silver nanoparticles in nanoliposomes could provide protection and targeting for the encapsulated or entrapped material. Nanoliposomes are biocompatible and biodegradable drug delivery systems with the ability to encapsulate both lipidsoluble and water-soluble compounds, as well as metal ions. Furthermore, nanoliposomes have been shown to be able to deliver encapsulated agents to target bacteria in vitro as well as in vivo. In this review, we present the use of nanoliposome-encapsulated silver nanoparticles as an efficient system for antibacterial applications.

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Enhanced Antibacterial Activity of Poly (dimethylsiloxane) Membranes by Incorporating SiO2 Microspheres Generated Silver Nanoparticles

Nanomaterials ◽

10.3390/nano9050705 ◽

2019 ◽

Vol 9 (5) ◽

pp. 705 ◽

Cited By ~ 4

Author(s):

Qihui Shen ◽

Yixuan Shan ◽

Yang Lü ◽

Peng Xue ◽

Yan Liu ◽

...

Keyword(s):

Silver Nanoparticles ◽

Bacterial Resistance ◽

Click Reaction ◽

Low Cost ◽

Thiol Group ◽

Water Contact ◽

Stabilizing Agents ◽

Wide Range ◽

Sio2 Microspheres

The nonspecific adsorption of proteins and bacteria on the surface of polydimethylsiloxane (PDMS) had been a serious concern in a wide range of applications, such as medical devices. In order to improve the anti-adhesive and antibacterial capability, bare silver nanoparticles (AgNPs, ~15 nm) were generated in-situ on their surface without extra reducing and stabilizing agents. The main reason for this was that the SiO2 microspheres that are covalent bonded to the bulked PDMS could not only generate AgNPs spontaneously but also insure that no AgNPs were released to the environment. Meanwhile, the thiol-group-functionalized SiO2 microspheres self-assembled on the surface of PDMS by thiol-vinyl click reaction without any impact on their biomedical applications. After the modification of SiO2 microspheres with AgNPs, the surface of PDMS showed a smaller water contact angle than before, and the adhesion and growth of E. coli and Bacillus subtilis were effectively inhibited. When the monolayer of SiO2 microspheres with AgNPs was assembled completely on the surface of PDMS, they present improved bacterial resistance performance (living bacteria, 0%). This approach offers an antibacterial and anti-adhesive surface bearing small and well-defined quantities of in-situ generated AgNPs, and it is a novel, green, simple, and low-cost technique to generate AgNPs on soft biomedical substrates.

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Influence of Surface Texture of Implants on Microorganism – A Review

Journal of Evolution of Medical and Dental Sciences ◽

10.14260/jemds/2021/430 ◽

2021 ◽

Vol 10 (28) ◽

pp. 2104-2107

Author(s):

Gayathri Karan Rajpurohit ◽

Arvina Rajasekar

Keyword(s):

Dental Implants ◽

Surface Texture ◽

Bacterial Resistance ◽

Bacterial Load ◽

Surface Modifications ◽

Bacterial Attachment ◽

Microbial Load ◽

Implant Surface ◽

Microbial Resistance ◽

Technical Solutions

BACKGROUND The development of endosseous osseointegrated dental implants has been very rapid over the past 20 years. The present literature review focuses on evaluating the various modifications done on the surface of dental implant and its influence on microorganisms. We wanted to review the evidence on the surface texture of implants and its influence on microorganisms. METHODS A Medline research was done, and all the information was gathered from various research articles. The keywords on the search pad were “implant”, “surface texture”, “surface modifications “, “biofilm”, “bacterial attachment”, “adhesion”, “microbes”, “antibacterial”, “acid etching”, “subtractive” and “additive” changes. The research publications were searched on Google Scholar and PubMed. Screening of studies which were eligible for the review, quality assessment, inclusion criteria, exclusion criteria and data extraction for all the endosseous implants with various surface modifications were checked. Verification of the information was conducted by two reviewers independently to eliminate any bias. The review article included systemic analysis, retrospective study and randomised trials. The results were all initially tabulated comparing the surface modifications with their effect on implant including bacterial resistance, osteogenic, osteoconductive etc. Based on the evidence the results were formulated, and the conclusion was made. RESULTS It’s clear from the evidence that there was no constancy in the results obtained. Each study believes in different techniques and different ideologies of the researcher to improve the microbial resistance either by coating or by surface modification. Due to the varying pattern of results, it is difficult to identify a definite reason for the microbial load over the implant. CONCLUSIONS There was no constancy in the results obtained. Overall, there are many technical solutions to avoid implant failure due to the bacterial load. These technical solutions exhibit a great potential when tried on preclinical models but there is a lack of clinical trial which hinders the achievement of any proper conclusion to build a standard protocol for the manufacturing of dental implants with structural modification. As implants are considered to be the most effective way to replace a missing tooth, standard technique with better surface texture is required to have good strength and better microbial resistance. KEY WORDS Surface Texture, Implant, Biofilm, Microbial Load, Modifications, Antimicrobial, Osseoconductive, Osseointegration

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