Evaluation of single-tube osmotic fragility as a screening test for thalassemia

2005 ◽  
Vol 79 (3) ◽  
pp. 198-201 ◽  
Author(s):  
Jason Chow ◽  
Lorraine Phelan ◽  
Barbara J. Bain
Keyword(s):  
Screening Test ◽  
Osmotic Fragility ◽  
Single Tube

scholarly journals Evaluation of Naked Eye Single Tube Red Cell Osmotic Fragility Test (NESTROFT) in Screening and Diagnosis of Beta Thalassaemia Trait in Pakistani Population

2021 ◽  
Keyword(s):  
Screening Test ◽  
Complete Blood Count ◽  
Osmotic Fragility ◽  
Rapid Screening ◽  
Red Cell ◽  
Pakistani Population ◽  
Single Tube ◽  
Beta Thalassaemia ◽  
Naked Eye ◽  
Thalassaemia Trait

Background: Beta thalassaemia is an inherited disease that is a cause of continuous burden on the affected families and the society. The objective of the study was to evaluate the efficacy of Naked Eye Single Tube Red Cell Osmotic Fragility Test (NESTROFT) for the detection of beta thalassaemia trait when used for the screening in Pakistani population. Methods: All participants requesting a complete blood count were included in the study. Informed consent was obtained. Naked Eye Single Tube Red Cell Osmotic Fragility Test was performed with freshly prepared 0.36% buffered saline. Turbidity in the tubes was an indication for individuals to be positive for beta thalassaemia trait. Hemoglobin electrophoresis was performed on all positive cases to confirm the sensitivity and the reliability of the screening test. Chi-Square test was used to test for any significant correlation between the different hematological parameters and other variables. Results: A total of 812 patients were included in the study from January 2017 to June 2017. The mean age of participants was 35.5 + 13.6 years. There were 351 (43.2%) male and 461 (56.7%) female participants. A 100% Naked Eye Single Tube Red Cell Osmotic Fragility Test sensitivity was obtained with 65 (8%) positive cases having HbA2 levels of more than 3.2% indicating their true positivity whereas, all 747 (92%) negative cases having HbA2 levels of less than 3.2% indicating their true negativity. Conclusion: NESTROFT was found and a rapid screening test for the identification of carriers of beta thalassaemia trait in our population.

scholarly journals Role of Naked Eye Single Tube Red Cell Osmotic Fragility Test (NESTROFT) in Detecting Beta-Thalassemia Trait

2018 ◽  
Vol 36 (4) ◽  
pp. 145-152 ◽  
Author(s):  
Asma Ferdousi ◽  
Mainuddin Ahmad ◽  
Jhulan Das Sharma ◽  
Rasheda Samad ◽  
AKM Zafar Ullah
Keyword(s):  
Predictive Value ◽  
Disease Incidence ◽  
Osmotic Fragility ◽  
Beta Thalassemia ◽  
Red Cell ◽  
College Hospital ◽  
Single Tube ◽  
Naked Eye ◽  
Thalassemia Trait

Thalassemia is one of the commonest inherited diseases in Bangladesh. The birth of a thalassemic child places considerable strain, not only on the affected child and its family but also on the community and the nation at large. To reduce the burden of the society and to reduce the disease incidence by providing genetic counseling, detection of carrier is important. The present study evaluates the role of ‘Naked Eye Single Tube Red Cell Osmotic Fragility Test’ (NESTROFT) in detecting ß-thalassemia trait. The current study is a cross sectional study done during the period of September 2008 to August 2009. The study subjects were sibs, parents and relatives of thalassemia patients of age more than 1 year attending Pediatric department of Chittagong Medical College Hospital. Sample size was 50. Here subjects with lowered osmotic fragility test were detected and later on Hb- electrophoresis was done. All the data were recorded and analyzed by SPSS programme. The Sensitivity, Specificity and predictive value of positive and negative tests were computed and they were 92.6%, 80%, 92.6% and 80% respectively. False positive cases were found. The present study found NESTROFT to be both sensitive and reasonably specific and of high negative predictive value. However, multicenter study with large sample is needed to recommend NESTROFT as a single screening test for detection of ß-thalassemia trait.J Bangladesh Coll Phys Surg 2018; 36(4): 145-152

scholarly journals Triple Lipid Screening Test: A Homogeneous Sequential Assay for HDL-Cholesterol, Total Cholesterol, and Triglycerides

2001 ◽  
Vol 47 (3) ◽  
pp. 532-539 ◽  
Author(s):  
Maureen L Sampson ◽  
Andrea Aubry ◽  
Gyorgy Csako ◽  
Alan T Remaley
Keyword(s):  
Total Cholesterol ◽  
Screening Test ◽  
Detection System ◽  
Hdl Cholesterol ◽  
Serum Lipoprotein ◽  
Single Tube ◽  
Lipid Screening ◽  
Homogeneous Assay ◽  
Artery Disease ◽  
Enzyme Detection

Abstract Background: The analysis of lipids in serum lipoprotein fractions is useful in assessing the risk for coronary artery disease, but it typically involves performing multiple tests. An automated single-tube assay, referred to as the triple lipid screening (TLS) test, can be used for measuring HDL-cholesterol (HDL-C), total cholesterol, and triglycerides (TGs) with no specimen pretreatment. Methods: The first part of the assay is based on a homogeneous assay for HDL-C that uses either an anti-apolipoprotein B antibody (TLS-A test) or a polyanion (TLS-B test) that blocks the enzymatic measurement of cholesterol on the non-HDL fraction. After the addition of deoxycholate, which solubilizes the unreacted cholesterol from the non-HDL fraction, the remaining cholesterol in the sample is subsequently measured enzymatically. Using the same enzyme detection system as the cholesterol assay, TGs are measured in the last step, after the addition of the enzymes for the TG assay. Results: The TLS assay (y) had acceptable analytic performance and compared favorably with standard tests (x) for each analyte: for HDL-C, TLS-A = 0.99x + 0.19 (R = 0.980); TLS-B = 1.00x − 0.15 (R = 0.974); for total cholesterol, TLS-A = 1.03x + 0.12 (R = 0.997); TLS-B = 1.07x − 0.30 (R = 0.965); and for TGs, TLS-A = 1.02x + 0.02 (R = 0.988); TLS-B = 1.04x − 0.28 (R = 0.980). Conclusions: The TLS test is a single-tube homogeneous assay for the analysis of all of the major serum lipoprotein fractions and can be used as a simple screening test for the detection of hyperlipidemia.

Validity of Naked Eye Single Tube Red Cell Osmotic Fragility Test for the Diagnosis of β-Thalassemia –A Case Control Study

2011 ◽  
Vol 24 (1) ◽  
pp. 1-5 ◽  
Author(s):  
MI Bari ◽  
LS Sharmin ◽  
PK Paul
Keyword(s):  
Negative Predictive Value ◽  
Predictive Value ◽  
Osmotic Fragility ◽  
Red Cell ◽  
College Hospital ◽  
Single Tube ◽  
Medical College ◽  
Naked Eye ◽  
Sensitivity Specificity ◽  
Control Study

This study was conducted at the department of paediatrics, Rajshahi Medical College Hospital over a period of 2 years to see the sensitivity, specificity, positive predictive value and negative predictive value of Naked Eye Single Tube Red cell Osmotic Fragility (NESTROF) test for the diagnosis of β-thalassemia. Fifty eight children with β- thalassemia were taken as case and 58 children were taken as control. Among the control 29 children were normal (without anaemia) and 29 children had anaemia other than β-thalassemia. Hemoglobin electrophoresis was done to all children to enroll them as case or control. Then NESTROF test was performed in all cases and controls. The data was analyzed by SPSS V.12. This study showed that sensitivity, specificity, positive and negative predictive value of NESTROF test was 96.55%, 72.41%, 77.77% and 95.45% respectively. TAJ 2011; 24(1): 1-5

Short-Form Application of the Screening Test of Spanish Grammar and the Northwestern Syntax Screening Test to Latino Children

1978 ◽  
Vol 9 (4) ◽  
pp. 220-235
Author(s):  
David L. Ratusnik ◽  
Carol Melnick Ratusnik ◽  
Karen Sattinger
Keyword(s):  
New York ◽  
Case Studies ◽  
Metropolitan Area ◽  
Screening Test ◽  
Normative Data ◽  
Short Form ◽  
Latino Children ◽  
Data Application ◽  
York Metropolitan Area ◽  
Multiple Regression Technique

Short-form versions of the Screening Test of Spanish Grammar (Toronto, 1973) and the Northwestern Syntax Screening Test (Lee, 1971) were devised for use with bilingual Latino children while preserving the original normative data. Application of a multiple regression technique to data collected on 60 lower social status Latino children (four years and six months to seven years and one month) from Spanish Harlem and Yonkers, New York, yielded a small but powerful set of predictor items from the Spanish and English tests. Clinicians may make rapid and accurate predictions of STSG or NSST total screening scores from administration of substantially shortened versions of the instruments. Case studies of Latino children from Chicago and Miami serve to cross-validate the procedure outside the New York metropolitan area.

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