Synthesis and wound healing performance of new water‐soluble chitosan derivatives

Hyaluronic acid (HA) has been known to play an important role in wound healing process. However, the effect of molecular weight (MW) of exogenously administered HA on the wound healing process has not been fully understood. In this study, we investigated HA with different MWs on wound healing process using human epidermal keratinocytes and dermal fibroblasts. Cell proliferation and migration ability were assessed by water soluble tetrazolium (WST) assay and wound scratch assay. We examined the effect of HA addition in a full-thickness wound model in mice and the gene expression related to wound healing. Proliferation and migration of HaCaT cells increased with the increase of MW and concentration of HA. Interleukin (IL-1β), IL-8 and vascular endothelial growth factor (VEGF) as well as matrix metalloproteinase (MMP)-9 and MMP-13 were significantly upregulated by high molecular weight (HMW) HA in keratinocytes. Together with VEGF upregulation and the observed promotion of HaCaT migration, HA with the MW of 2290 kDa may hold potential to improve re-epithelialization, a critical obstacle to heal chronic wounds.

Download Full-text

Water-soluble chitosan derivatives as a BACE1 inhibitor

Bioorganic & Medicinal Chemistry ◽

10.1016/j.bmc.2005.07.004 ◽

2005 ◽

Vol 13 (23) ◽

pp. 6551-6555 ◽

Cited By ~ 61

Author(s):

Jae-Young Je ◽

Se-Kwon Kim

Keyword(s):

Water Soluble ◽

Chitosan Derivatives ◽

Bace1 Inhibitor

Download Full-text

Chitin and Chitosan Derivatives for Wound-Healing Applications

Chitin and Chitosan Derivatives ◽

10.1201/b15636-20 ◽

2013 ◽

pp. 259-274

Keyword(s):

Wound Healing ◽

Chitosan Derivatives ◽

Chitin And Chitosan

Download Full-text

Wound Healing-Promoting and Melanogenesis-Inhibiting Activities of Angelica polymorpha Maxim. Flower Absolute In Vitro and Its Chemical Composition

Molecules ◽

10.3390/molecules26206172 ◽

2021 ◽

Vol 26 (20) ◽

pp. 6172

Author(s):

Su-Yeon Lee ◽

Kyung-Jong Won ◽

Do-Yoon Kim ◽

Mi-Jung Kim ◽

Yu-Rim Won ◽

...

Keyword(s):

Wound Healing ◽

Collagen Synthesis ◽

Biological Activities ◽

Melanoma Cells ◽

Skin Wound ◽

Water Soluble ◽

Boyden Chamber ◽

Tetrazolium Salt ◽

Skin Wound Healing ◽

Skin Whitening

Angelica polymorpha Maxim. (APM) is used in traditional medicine to treat chronic gastritis, rheumatic pain, and duodenal bulbar ulcers. However, it is not known whether APM has epidermis-associated biological activities. Here, we investigated the effects of APM flower absolute (APMFAb) on responses associated with skin wound healing and whitening using epidermal cells. APMFAb was obtained by solvent extraction and its composition was analyzed by GC/MS. Water-soluble tetrazolium salt, 5-bromo-2′-deoxyuridine incorporation, Boyden chamber, sprouting, and enzyme-linked immunosorbent assays and immunoblotting were used to examine the effects of APMFAb on HaCaT keratinocytes and B16BL6 melanoma cells. APMFAb contained five compounds and induced keratinocyte migration, proliferation, and type IV collagen synthesis. APMFAb also induced the phosphorylations of ERK1/2, JNK, p38 mitogen-activated protein kinase, and AKT in keratinocytes. In addition, APMFAb decreased serum-induced B16BL6 cell proliferation and inhibited tyrosinase expression, melanin contents, and microphthalmia-associated transcription factor expression in α-melanocyte-stimulating hormone-stimulated B16BL6 cells. These findings demonstrate that APMFAb has beneficial effects on skin wound healing by promoting the proliferation, migration, and collagen synthesis of keratinocytes and on skin whitening by inhibiting melanin synthesis in melanoma cells. Therefore, we suggest that APMFAb has potential use as a wound healing and skin whitening agent.

Download Full-text

Searching for synergistic effects of low-molecular weight chitosan derivatives, chitosan and copper nanoparticles for wound healing ointment

Advances in Natural Sciences Nanoscience and Nanotechnology ◽

10.1088/2043-6262/ac2b98 ◽

2021 ◽

Vol 12 (3) ◽

pp. 035016

Author(s):

Natalya Nikolaevna Glushchenko ◽

Olga Alexandrovna Bogoslovskaya ◽

Balzhima Tsyrendorzhievna Shagdarova ◽

Alla Victorovna Il’ina ◽

Irina Pavlovna Olkhovskaya ◽

...

Keyword(s):

Molecular Weight ◽

Wound Healing ◽

Copper Nanoparticles ◽

Synergistic Effects ◽

Low Molecular Weight ◽

Chitosan Derivatives ◽

Low Molecular Weight Chitosan

Download Full-text

Fine particulate matter PM2.5 generated by building demolition increases the malignancy of breast cancer MDA-MB-231 cells

10.21203/rs.2.23043/v1 ◽

2020 ◽

Author(s):

Chun-Wen Cheng ◽

Gwo-Tarng Sheu ◽

Jing-Shiuan Chou ◽

Pei-Han Wang ◽

Yu-Chun Cheng ◽

...

Keyword(s):

Breast Cancer ◽

Wound Healing ◽

Cancer Cells ◽

Fine Particulate Matter ◽

Water Soluble ◽

Migration And Invasion ◽

Control Group ◽

Increased Risk ◽

Freeze Dried ◽

Building Demolition

Abstract Background PM2.5 is associated with increased risk of mortality for a variety of cancers and all subjects, including breast cancer in females, and lung cancer in males. This study investigates the effects of water-extracted PM2.5 on a triple-negative breast cancer (TNBC) cell line, MDA-MB-231, by sampling suspended particulates around a building demolition site.Methods PM2.5 particles were obtained using a high-flow TISCH sampler. Being water-soluble, they were extracted from sampled filters using an ultrasonic oscillator and then freeze-dried. The heavy metal components of soluble PM2.5 particle was analyzed by ICP-MS. Cell viability was evaluated by MTT assay for cells that were exposed to PM2.5. Wound healing and transwell cell migration and invasion assays were used to measure cell motility and the invasiveness of cancer cells that had been exposed to PM2.5 into a chemo-attractant substance. Possible mechanisms of cancer malignancy were analyzed by Western blot analysis.Results The results revealed that nearby PM2.5 concentrations increased significantly during the deconstruction of buildings, and the Cd, Cu, Pb, Zn and Cr contents of soluble PM2.5 also significantly increased. Following exposure to PM2.5, the survival rate of breast cancer cells was significantly higher than that of the control group. Soluble PM2.5-treated cells also had a higher migration capacity, as determined by wound healing and transwell migration assays. The signaling pathway of FAK/PI3K/AKT proteins was more activated in PM2.5-treated cells than the control group. The data show that increased levels of Aurora B and Bcl-2 were associated with cell proliferation. Elevated levels of cathepsins D, β-catenin, N-cadherin, vimentin and MMP-9 were associated with breast cancer cell metastasisConclusion Soluble PM2.5 that is generated in building demolition may have a role in the promotion/progression of surviving in TNBC cells, increasing the malignancy of breast cancer. The prevention of environmental PM2.5 from deconstruction is strongly recommended.

Download Full-text