Purification, characterization and antifungal activity of Bacillus cereus strain NK91 chitinase from rhizospheric soil samples of Himachal Pradesh

2021 ◽  
Author(s):  
Nirja Thakur ◽  
Amarjit K Nath ◽  
Anjali Chauhan ◽  
Rakesh Gupta
Keyword(s):  
Antifungal Activity ◽  
Bacillus Cereus ◽  
Himachal Pradesh ◽  
Soil Samples ◽  
Rhizospheric Soil

Sidrophore Production and Phosphate Solubilization by Bacillus cereus and Pseudomonas fluorescens Isolated from Iraqi Soils and Soil Characterization

2018 ◽  
Vol 9 (4) ◽  
Author(s):  
Zaid Raad Abbas ◽  
Aqeel Mohammed Majeed Al-Ezee ◽  
Sawsan H
Keyword(s):  
Bacillus Cereus ◽  
Pseudomonas Fluorescens ◽  
Phosphate Solubilization ◽  
Bacterial Species ◽  
Nutrient Status ◽  
Bacterial Count ◽  
Soil Samples ◽  
Phosphate Solubilizing Bacteria ◽  
Clear Zone ◽  
Zone Formation

This study was conducted to explore the ability of Pseudomonas fluorescens and Bacillus cereus to solubilizing a phosphate in soil for enhancing the planting growth and, its relation with soill characterization. The isolates were identified as P.fluorescens and B. cereus using convential analysis and, its phosphate solubilization ability and sidrophore was shown by the clear zone formation on National Botanical Research Institute���s Phosphate medium. Moreover, Pseudomonas fluorescens isolates (n = 9) and three of B. cereus isolated from agricultural area in Baghdad university, Mustansiriyah university and Diyala bridge. Results displayed that bacterial count were varied in soil samples according to their region, and ranging from 30 to 60 *10 2 CFU/g in Baghdad university soil to 10���20 *10 2 CFU/g in Mustansiriyah university soil, the Baghdad soil macronutrient which included: NH4, NO3, P, and K were, 8.42, 20.53, 19.09, 218.73 respectively, While the physio analysis revealed that the mean of pH was 7.3 and EC was 8.63. on the other hand the micronutrient analysis indicated that the soil samples were included Ca, Fe, Mn, Zn and Cu which gave their mean 5025.9, 8.9, 4.9, 0.5 and 1.5 respectevily. Results revealed that all isolated bacteria (9 isolates of P.fluorescens and three isolates of B. cereus gave ahalo zone which mean their ability to be phosphate solubilizing bacteria at 100%. Results revealed that all isolated bacteria were detected a ability to produce high levels from chelating agents (siderophores)) by P.fluorescens and. B cereus at 100%, when appeared ahalo clear zone. Furthermore, the high levels of phosphate solubilization and siderophore production were grouped in bacterial species isolated from Iraqi soils. might be attributed to many soil factors such as soil nutrient status, soil acidity, water content, organic matter and soil enzyme activities.

scholarly journals Antifungal activity of several isolates of Trichoderma against Cladosporium and Botrytis

2018 ◽  
Vol 8 (1) ◽  
pp. 88-91 ◽  
Author(s):  
M. Skaptsov ◽  
S. Smirnov ◽  
M. Kutsev ◽  
O. Uvarova ◽  
T. Sinitsyna ◽  
...  
Keyword(s):  
Antifungal Activity ◽  
Trichoderma Harzianum ◽  
Its Region ◽  
Botanical Garden ◽  
Antagonistic Activity ◽  
Antagonistic Effect ◽  
Soil Samples ◽  
Morphological Observation ◽  
The South

<p><em>Trichoderma</em> isolates (SSBGT07, SSBGT08, SSBGT09, SSBGT10) were isolated from the soil samples of the South-Siberian Botanical Garden and identified using morphological observation and ITS region analysis as <em>Trichoderma harzianum</em>, <em>T. asperellum, T. ghanense</em>, and <em>T. longibranchiatum</em>. Antagonistic activity against <em>Cladosporium </em>sp. and<em> Botrytis </em>sp. was evaluated <em>in vitro</em>. All isolates showed antagonistic effect by competition against <em>Cladosporium </em>sp. <em>T. asperellum </em>and <em>T. longibranchiatum</em> showed antagonism against <em>Botrytis </em>sp. All isolates showed hyper sporulation on the sclerotia of <em>Botrytis</em> sp. (except the <em>T. ghanense</em>) and colonies of the <em>Cladosporium</em> sp. Our study provides new isolates that affect the <em>Cladosporium </em>sp. and<em> Botrytis </em>sp.</p>

scholarly journals The Possible Transmission and Potential Enterotoxicity of Bacillus cereus on Lettuce Farms in Five Chinese Provinces

2021 ◽  
Vol 12 ◽  
Author(s):  
Yang Qu ◽  
Chao Wei ◽  
Xiaohang Dai ◽  
Yalong Bai ◽  
Xin Zhao ◽  
...  
Keyword(s):  
Bacillus Cereus ◽  
Potential Risk ◽  
Trace Analysis ◽  
Multilocus Sequence Typing ◽  
Soil Samples ◽  
Foodborne Diseases ◽  
Human Pathogen ◽  
Toxin Genes ◽  
Positive Rate ◽  
Sequence Types

Bacillus cereus is a well-characterized human pathogen that produces toxins associated with diarrheal and emetic foodborne diseases. To investigate the possible transmission of B. cereus on lettuce farms in China and determine its enterotoxicity, (I) a total of 524 samples (lettuce: 332, soil: 69, water: 57, manure: 57, pesticide: 9) were collected from 46 lettuce farms in five Chinese provinces, (II) multilocus sequence typing (MLST) was used to classify B. cereus isolates and for trace analysis, and (III) the presence of toxin genes and enterotoxins (Hbl and Nhe) was detected in 68 strains. The results showed that one hundred and sixty-one lettuce samples (48.5%) tested positive for B. cereus at levels ranging from 10 to 5.3 × 104 CFU/g. Among the environmental sample categories surveyed, the highest positive rate was that of the pesticide samples at 55.6%, followed by soil samples at 52.2% and manure samples at 12.3%. Moreover, one hundred isolates of B. cereus yielded 68 different sequence types (STs) and were classified into five phylogenetic clades. Furthermore, Nhe toxin genes (nheA, nheB, nheC) were broadly distributed and identified in all 68 strains (100%), while Hbl toxin genes (hblA, hblC, hblD) were present in 61 strains (89.7%), entFM was detected in 62 strains (91.2%), and cytK was found in 29 strains (42.6%). All strains were negative for ces. As for the enterotoxin, Nhe was observed in all 68 isolates carrying nheB, while Hbl was present in 76.5% (52/68) of the strains harboring hblC. This study is the first report of possible B. cereus transmission and of its potential enterotoxicity on lettuce farms in China. The results showed that soil and pesticides are the main sources of B. cereus on lettuce farms in China, and the possible transmission routes are as follows: soil-lettuce, manure-lettuce, pesticide-lettuce, manure-soil-lettuce, and water-manure-soil-lettuce. Furthermore, the B. cereus isolates, whether from lettuce or the environment, pose a potential risk to health.

Nutrition carbonée et étude taxonomique de Bacillus megaterium et B. cereus

1980 ◽  
Vol 26 (7) ◽  
pp. 778-786 ◽  
Author(s):  
F. Pichinoty ◽  
M. Durand ◽  
M. Mandel
Keyword(s):  
Bacillus Cereus ◽  
Bacillus Megaterium ◽  
Numerical Taxonomy ◽  
Soil Samples ◽  
Mean Values ◽  
Enrichment Cultures ◽  
Group A ◽  
Group B ◽  
Peptone Medium ◽  
Selective Technique

Ten of the 14 strains of Bacillus cereus studied were isolated from pasteurized soil samples by a new selective technique which consists of the use of anaerobic enrichment cultures in peptone medium containing 0.5% KNO3. The 19 strains of B. megaterium use the following 21 sources of carbon and energy: D-glucose, D-galactose, D-fructose, D-mannose, D-glucosamine, D-gluconate, D-ribose, sucrose, cellobiose, maltose, raffinose, D-mannitol, glycerol, glycerate, pyruvate, fumarate, trans-aconitate, DL-aspartate, asparagine, L-glutamate, and L-glutamine. The 14 strains of B. cereus use the following 11 compounds: D-glucose, D-fructose, D-mannose, D-glucosamine, D-ribose, maltose, trehalose, glycerol, acetate, pyruvate, and L-glutamine. The eight strains of B. cereus var. mycoides use the following nine compounds: D-glucose, salicin, D-ribose, cellobiose, maltose, trehalose, glycerol, acetate, and pyruvate. Numerical taxonomy based on 151 characters shows that the 41 strains form two distinct groups. Group A includes the 19 strains of B. magateriurn; group B includes the 14 strains of B. cereus and the 8 strains of B. cereus var. mycoides. The guanine + cytosine content of the DNA of each strain was determined. The following mean values were obtained: B. megaterium, 40.6% ± 0.8; B. cereus, 38% ± 0.9; B. cereus var. mycoides, 38.4% ± 1.1.

Radopholoides japonicus n. sp. (Nematoda: Pratylenchidae) found in rhizosphere soil associated with Podocarpus macrophyllus from Japan

Nematology ◽  
2017 ◽  
Vol 19 (9) ◽  
pp. 1095-1105
Author(s):  
Munawar Maria ◽  
Jianfeng Gu ◽  
Yiwu Fang ◽  
Jie He ◽  
Pablo Castillo ◽  
...  
Keyword(s):  
Rhizosphere Soil ◽  
Soil Samples ◽  
Molecular Characterisation ◽  
Maximum Likelihood Analysis ◽  
Rhizospheric Soil ◽  
Long Tail ◽  
Likelihood Analysis ◽  
Lateral Lines ◽  
Podocarpus Macrophyllus ◽  
Partial 18S

Radopholoides japonicus n. sp. isolated from rhizospheric soil samples associated with Podocarpus macrophyllus imported from Japan into Ningbo Port of China is described and illustrated. It is characterised by four lateral lines, lip region low, convex with three annuli. Stylet 16.5-18.5 μm long with anteriorly sloping rounded knobs. Excretory pore at the level of pharyngo-intestinal junction. Vulva posteriorly located, vulval lips not protruding, spermatheca squarish. Post-vulval uterine sac 35-44 μm long. Tail conoid with broadly rounded terminus, phasmid 6-8 annuli posterior to anus. Among five species of genus Radopholoides, the new species is close to R. antoni but can be differentiated from it by lip and tail morphology. This is the first Radopholoides species that provided detailed morphological and molecular characterisation. Maximum Likelihood analysis using the Shimodaira-Hasegawa test for the validity of Radopholoides was performed and showed the validity of the genus using the partial 18S and D2-D3 expansion segments of 28S.

scholarly journals Bacillus cereus: public health burden associated with ready-to-eat foods in Himachal Pradesh, India

2020 ◽  
Vol 57 (6) ◽  
pp. 2293-2302
Author(s):  
Neha Rana ◽  
Ashok Kumar Panda ◽  
Nina Pathak ◽  
Tania Gupta ◽  
Sidharath Dev Thakur
Keyword(s):  
Public Health ◽  
Bacillus Cereus ◽  
Himachal Pradesh ◽  
Public Health Burden ◽  
Health Burden
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