A study on Hydrocarbon Degradation by Biosurfactant Producing Bacillus cereus in Oil Contaminated Soil Samples

2016 ◽  
Vol 2 (4) ◽  
Author(s):  
S. Janaki ◽  
S. Thenmozhi ◽  
R Muthumari
Keyword(s):  
Bacillus Cereus ◽  
Contaminated Soil ◽  
Soil Samples ◽  
Hydrocarbon Degradation

scholarly journals Biodegradation of used Engine Oil Contaminated Site using Individual and Mixed bacterial isolates

2020 ◽  
pp. 38-45
Author(s):  
Muh'd A. J. ◽  
Sa'adatu A.Y. ◽  
Surayya M. M. ◽  
Sa'adatu M. I. ◽  
Nafisa B. ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Bacillus Cereus ◽  
Contaminated Soil ◽  
Bacterial Species ◽  
Soil Samples ◽  
Engine Oil ◽  
Contaminated Site ◽  
Bacterial Isolates ◽  
The World ◽  
Degradation Ability

Oil is most widely distributed source of energy in the world and large-scaled environmental pollutant. Oil, oil products, and oil containing industrial waste pollution is ranked second place after radioactive pollution on account of their harmful action to ecosystems. Contamination of soil by organic chemicals (mostly hydrocarbons) is prevalent in oil producing and industrialized countries of the world. Biodegradation, a strategy that uses biological means (i.e microbes) to degrade, stabilize and remove soil contaminants is an alternative green technology remediation of hydrocarbon contaminated soil. This study was carried out to isolate and screen Bacteria capable of degrading used engine oil from oil contaminated site. Soil samples were collected from oil contaminated site. Bacterial species were isolated from the collected soil samples by serial dilution and agar methods. Different bacterial species were isolated but only four were oil degrading isolates. The identity of the various genera of bacterial contaminants were determined by a combination of cultural, morphological as well as preliminary biochemical characteristics of the isolates. The four oil degrading bacteria (AJ1, AJ2, AJ3 and AJ4) were preliminarily identified as Bacillus cereus, Pseudomonas aeruginosa, Bacillus subtillis and Micrococcus spp respectively. The degradation ability of the bacterial isolates was screened and maximum degradation was recorded by AJ 5 (Mixed culture) with 66.9 %, followed by Bacillus cereus (50.3 %), Bacillus subtilis (44 %), Pseudomonas aeruginosa (37.9 %) and the least was seen in Micrococcus spp (35.3 %). These findings revealed that some bacteria species are capable of utilizing the oil and used it as sole source of carbon and energy and the mixed consortia of the bacteria have rapid degradation ability. Biological degradation of hydrocarbon contaminated soil offers a better and more environmentally friendly technique that if properly explored can bring our environment into a better place for both plant and animal.

Sidrophore Production and Phosphate Solubilization by Bacillus cereus and Pseudomonas fluorescens Isolated from Iraqi Soils and Soil Characterization

2018 ◽  
Vol 9 (4) ◽  
Author(s):  
Zaid Raad Abbas ◽  
Aqeel Mohammed Majeed Al-Ezee ◽  
Sawsan H
Keyword(s):  
Bacillus Cereus ◽  
Pseudomonas Fluorescens ◽  
Phosphate Solubilization ◽  
Bacterial Species ◽  
Nutrient Status ◽  
Bacterial Count ◽  
Soil Samples ◽  
Phosphate Solubilizing Bacteria ◽  
Clear Zone ◽  
Zone Formation

This study was conducted to explore the ability of Pseudomonas fluorescens and Bacillus cereus to solubilizing a phosphate in soil for enhancing the planting growth and, its relation with soill characterization. The isolates were identified as P.fluorescens and B. cereus using convential analysis and, its phosphate solubilization ability and sidrophore was shown by the clear zone formation on National Botanical Research Institute���s Phosphate medium. Moreover, Pseudomonas fluorescens isolates (n = 9) and three of B. cereus isolated from agricultural area in Baghdad university, Mustansiriyah university and Diyala bridge. Results displayed that bacterial count were varied in soil samples according to their region, and ranging from 30 to 60 *10 2 CFU/g in Baghdad university soil to 10���20 *10 2 CFU/g in Mustansiriyah university soil, the Baghdad soil macronutrient which included: NH4, NO3, P, and K were, 8.42, 20.53, 19.09, 218.73 respectively, While the physio analysis revealed that the mean of pH was 7.3 and EC was 8.63. on the other hand the micronutrient analysis indicated that the soil samples were included Ca, Fe, Mn, Zn and Cu which gave their mean 5025.9, 8.9, 4.9, 0.5 and 1.5 respectevily. Results revealed that all isolated bacteria (9 isolates of P.fluorescens and three isolates of B. cereus gave ahalo zone which mean their ability to be phosphate solubilizing bacteria at 100%. Results revealed that all isolated bacteria were detected a ability to produce high levels from chelating agents (siderophores)) by P.fluorescens and. B cereus at 100%, when appeared ahalo clear zone. Furthermore, the high levels of phosphate solubilization and siderophore production were grouped in bacterial species isolated from Iraqi soils. might be attributed to many soil factors such as soil nutrient status, soil acidity, water content, organic matter and soil enzyme activities.

High Parasitic Contamination of Soil Samples in the North of Iran: a Potential Risk of Parasitic Infection for Tourists

2020 ◽  
Vol 20 ◽  
Author(s):  
Beheshteh Haghparast-kenari ◽  
Tooran Nayeri ◽  
Shahabeddin Sarvi ◽  
Mohammad Taghi Rahimi ◽  
Ehsan Ahmadpour ◽  
...  
Keyword(s):  
Contaminated Soil ◽  
Urban Areas ◽  
Intestinal Parasites ◽  
Parasitic Infection ◽  
Animal Husbandry ◽  
Soil Samples ◽  
Human Society ◽  
Public Parks ◽  
The North ◽  
North Of Iran

Background: Soil-transmitted parasites (STPs) are significant intestinal parasites that infect humans and animals and impose considerable burdens on human society and animal husbandry industries. Therefore, the present study aimed to determine the prevalence of parasitic elements of soil samples collected from the north of Iran. Methods: A total of 256 soil samples were collected from public parks, public places, vegetable gardens, sand heaps, and shadow areas near houses in the north of Iran and examined using the sucrose flotation method. Results: Out of 256 examined samples, 131 (51.2%) ones showed parasitic contamination including larvae (43%), oocysts (14.1%), and different eggs (6.6%). According to the results, the most and least common parasites observed in the samples were larvae (43%), as well as Toxascaris leonina, and Trichuris trichiura (0.4%), respectively. Moreover, the most and least contaminated locations were sand heaps (62.5%) and shadow areas near houses (45.6%), respectively. Regarding cities, Behshahr (68.3%) and Sari (67.5%) had the highest contaminated soil samples, whereas Chalus (37.5%) showed the lowest contamination. On the other hand, rural samples showed more contamination, compared to urban areas (P< 0.05). Conclusion: The findings of the present study indicate that the overall prevalence of STPs in examined areas and highly contaminated soil samples can be considered as a potential source of human contamination particularly tourists with STPs.

Evaluation of Nanotechniques and Conventional Techniques for the Removal of Dioxins

2018 ◽  
Vol 9 (1) ◽  
pp. 79-84
Author(s):  
Vaishali V. Shahare ◽  
Rajni Grover ◽  
Suman Meena
Keyword(s):  
Soil Sample ◽  
Human Health ◽  
Surface Area ◽  
Contaminated Soil ◽  
Conventional Treatment ◽  
Volume Ratio ◽  
Soil Samples ◽  
Dioxins And Furans ◽  
Palladized Iron ◽  
The Impact

Background: The persistent dioxins/furans has caused a worldwide concern as they influence the human health. Recent research indicates that nonmaterial may prove effective in the degradation of Dioxins/furans. The nanomaterials are very reactive owing to their large surface area to volume ratio and large number of reactive sites. However, nanotechnology applications face both the challenges and the opportunities to influence the area of environmental protection. Objective: i) To study the impact of oil mediated UV-irradiations on the removal of 2,3,7,8-TCDD, 2,3,7,8-TCDF, OCDD and OCDF in simulated soil samples. ii) To compare the conventional treatment methods with the modern available nanotechniques for the removal of selected Dioxins/furans from soil samples. Methods: The present work has investigated an opportunity of the degradation of tetra and octachlorinated dioxins and furans by using oil mediated UV radiations with subsequent extraction of respective dioxins/furans from soils. The results have been compared with the available nanotechniques. Results: The dioxin congeners in the simulated soil sample showed decrease in concentration with the increase in the exposure time and intensity of UV radiations. The dechlorination of PCDD/Fs using palladized iron has been found to be effective. Conclusion: Both the conventional methods and nanotechnology have a dramatic impact on the removal of Dioxins/furans in contaminated soil. However, the nanotechniques are comparatively costlier and despite the relatively high rates of PCDDs dechlorination by Pd/nFe, small fraction of the dioxins are recalcitrant to degradation over considerable exposure times.

scholarly journals Factorial design study of total petroleum contaminated soil treatment using land farming technique

2021 ◽  
Vol 31 (1) ◽  
Author(s):  
Ehizonomhen S. Okonofua ◽  
Kayode H. Lasisi ◽  
Eguakhide Atikpo
Keyword(s):  
Moisture Content ◽  
Factorial Design ◽  
Contaminated Soil ◽  
Soil Samples ◽  
Optimization Techniques ◽  
Cow Dung ◽  
Optimum Point ◽  
Input Parameters ◽  
Substrate Moisture ◽  
Land Farming

AbstractLand farming technique was used to treat hydrocarbon contaminated soil collected from a crude oil spill sites in Edo State, Nigeria. Calibrated standard auger was used to collect soil samples from the site at depth below 30 cm. The samples were characterized and classified. Cow dung and NPK fertilizer were added as additives to complement the nutriments of the soil samples before total petroleum hydrocarbon (TPH) quantification and remediation procedures. Factorial design was applied to vary the input parameters such as pH, mass of substrate, moisture content and turning times of land farming so to ascertain the optimal conditions for the procedure. The result revealed that the in-situ TPH value was 5000 mg kg− 1 on the average and after 90 d of treatment, TPH reduced to 646 mg kg− 1. The turning rate, pH, moisture content and mass of substrate hade 83, 4.36, 0.48 and 0.046% contribution, respectively, for the degradation process using land farming treatment. Numerical optimization techniques applied in the optimum point for land farming input parameters to achieve predicted maximum removal of 99% were evaluated as pH, mass of substrate, moisture content and turning rate to be 6.01, 1 kg, 10% and 5 times in a week, respectively. TPH removed at this optimum point was 98% reducing from 5000 to 636 mg kg− 1. The high coefficient of determination (r2 = 0.9865) as observed in the closeness of predicted and experimental values reflects the reliability of the model and hence, land farming practice with close attention on turning rate as revealed by this study, is recommended for TPH contaminated soil remediation.

scholarly journals Behavior of Eucalyptus grandis and E. cloeziana seedlings grown in arsenic-contaminated soil

2010 ◽  
Vol 34 (3) ◽  
pp. 985-992 ◽  
Author(s):  
Roseli Freire Melo ◽  
Luiz Eduardo Dias ◽  
Jaime Wilson Vargas de Mello ◽  
Juraci Alves Oliveira
Keyword(s):  
Root System ◽  
Plant Height ◽  
Contaminated Soil ◽  
Dry Matter ◽  
Eucalyptus Grandis ◽  
Soil Samples ◽  
Mining Activities ◽  
Polluted Soils ◽  
Interveinal Chlorosis ◽  
Apical Bud

Arsenic has been considered the most poisonous inorganic soil pollutant to living creatures. For this reason, the interest in phytoremediation species has been increasing in the last years. Particularly for the State of Minas Gerais, where areas of former mining activities are prone to the occurrence of acid drainage, the demand is great for suitable species to be used in the revegetation and "cleaning" of As-polluted areas. This study was carried out to evaluate the potential of seedlings of Eucalyptus grandis (Hill) Maiden and E. cloeziana F. Muell, for phytoremediation of As-polluted soils. Soil samples were incubated for a period of 15 days with different As (Na2HAsO4) doses (0, 50, 100, 200, and 400 mg dm-3). After 30 days of exposure the basal leaves of E. cloeziana plants exhibited purple spots with interveinal chlorosis, followed by necrosis and death of the apical bud at the 400 mg dm-3 dose. Increasing As doses in the soil reduced root and shoot dry matter, plant height and diameter in both species, although the reduction was more pronounced in E. cloeziana plants. In both species, As concentrations were highest in the root system; the highest root concentration was found in E. cloeziana plants (305.7 mg kg-1) resulting from a dose of 400 mg dm-3. The highest As accumulation was observed in E. grandis plants, which was confirmed as a species with potential for As phytoextraction, tending to accumulate As in the root system and stem.

scholarly journals MICROBIAL DIVERSITY OF PESTICIDE CONTAMINATED SOIL SAMPLES FROM TALGAR REGION

2021 ◽  
Vol 86 (1) ◽  
Author(s):  
G. Zh. Abdieva ◽  
P. S. Ualieva ◽  
А. М. Мalik ◽  
N. S. Akimbekov ◽  
А. N. Eshmukhanbet ◽  
...  
Keyword(s):  
Microbial Diversity ◽  
Contaminated Soil ◽  
Soil Samples
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