Ultrasound‐Assisted Enzyme‐Catalyzed Hydrolysis of Collagen to Produce Peptides With Biomedical Potential: Collagenase From Aspergillus terreus UCP1276

2019 ◽  
Vol 41 (2) ◽  
pp. 113-120
Author(s):  
Romualdo B. Costa‐Junior ◽  
Romero M. P. Brandão‐Costa ◽  
Wendell W. C. Albuquerque ◽  
Juanize M. S. Batista ◽  
Raquel B. Pedrosa ◽  
...  
Keyword(s):  
Aspergillus Terreus ◽  
Ultrasound Assisted ◽  
Hydrolysis Of ◽  
Biomedical Potential

Ultrasound assisted enzymatic hydrolysis of starch catalyzed by glucoamylase: Investigation on starch properties and degradation kinetics

2017 ◽  
Vol 175 ◽  
pp. 47-54 ◽  
Author(s):  
Danli Wang ◽  
Xiaobin Ma ◽  
Lufeng Yan ◽  
Thunthacha Chantapakul ◽  
Wenjun Wang ◽  
...  
Keyword(s):  
Enzymatic Hydrolysis ◽  
Degradation Kinetics ◽  
Starch Properties ◽  
Ultrasound Assisted ◽  
Hydrolysis Of

Detection of cellulase inhibitor in the wheat bran culture of Aspergillus terreus

1981 ◽  
Vol 27 (12) ◽  
pp. 1334-1340 ◽  
Author(s):  
S. N. Sinha ◽  
B. L. Ghosh ◽  
S. N. Ghose
Keyword(s):  
Molecular Weight ◽  
Wheat Bran ◽  
Filter Paper ◽  
Aspergillus Terreus ◽  
Defined Medium ◽  
Low Molecular Weight ◽  
Chemically Defined Medium ◽  
Plant Origin ◽  
First Time ◽  
Hydrolysis Of

The presence of a cellulase inhibitor in the wheat bran culture of a fungus is reported for the first time. The inhibitor has a low molecular weight and is relatively stable to heat. It is absent from wheat bran and is not produced in a chemically defined medium. Unlike cellulase inhibitors of plant origin, this inhibitor is not a polyphenol. It inhibits the hydrolysis of cotton to a greater degree than that of filter paper or carboxymethylcellulose. In addition to inhibiting Aspergillus terreus cellulase, it also inhibits a variety of commercial cellulases.

Ultrasound-assisted enzymatic hydrolysis of cassava waste to obtain fermentable sugars

2013 ◽  
Vol 115 (1) ◽  
pp. 1-6 ◽  
Author(s):  
Eloisa X. Leaes ◽  
Eduardo Zimmermann ◽  
Matheus Souza ◽  
Adriana P. Ramon ◽  
Ethiane T. Mezadri ◽  
...  
Keyword(s):  
Enzymatic Hydrolysis ◽  
Fermentable Sugars ◽  
Ultrasound Assisted ◽  
Hydrolysis Of ◽  
Cassava Waste

scholarly journals Ultrasound Assisted Pretreatments Applied to Cupuaçu Husk (Theobroma grandiflorum) from Brazilian Legal Amazon for Biorefinery Concept

2022 ◽  
Author(s):  
Nicole Marasca ◽  
Isabella Cardoso ◽  
Magale Rambo ◽  
Daniel Bertuol ◽  
Michele Rambo ◽  
...  
Keyword(s):  
Native Species ◽  
Liquid Fraction ◽  
Value Added ◽  
X Ray Diffraction ◽  
X Ray ◽  
Aqueous Acid ◽  
Ultrasound Assisted ◽  
Hydrolysis Of ◽  
Pretreated Biomass ◽  
Value Added Products

Cupuaçu husk (CH) is the waste of a common fruit from a native species of the Brazilian Legal Amazon. The current study investigated the influence of ultrasound (US) combined with aqueous, acid, alkaline, and ionic liquid (IL) pretreatments on the chemical and physical aspects of CH and the yield of chemical platforms production, 5-hydroxymethylfurfural (HMF) and furfural (FF), using IL. Scanning electron microscopy, X-ray diffraction (XRD) and Fourier transform infrared spectroscopy were used to feature the raw and pretreated biomass. The highest levels of glucose (9.90 g L-1) were observed in the liquid fraction resulting from the acid + US pretreatment followed by acid hydrolysis. The IL + US pretreatment recorded the best performance in removing lignin. Based on XRD analyses, ultrasound increased crystallinity of all pretreated samples as a result of the removal of cellulose’s amorphous fraction. However, it promoted accessibility to adopted reagents by increasing biomass exposure due to cavitation. The best yields of HMF and FF were recorded from hydrolysis of the solid fraction resulting from the acid + US (12.94%) and alkaline + US (48.84%) pretreatment, respectively. These results indicate satisfactory performance of ultrasound assisted pretreatments to the simplified and economic conversion of biomass into value-added products.

scholarly journals Ultrasound-Assisted Ultra-Mild-Acid Hydrolisis of -Carrageenan

REAKTOR ◽  
2018 ◽  
Vol 17 (4) ◽  
pp. 191
Author(s):  
Ratnawati Ratnawati ◽  
Nita Indriyani
Keyword(s):  
Molecular Weight ◽  
Acid Hydrolysis ◽  
Average Molecular Weight ◽  
Weight Fraction ◽  
Number Average Molecular Weight ◽  
Hydrolysis Process ◽  
Higher Temperature ◽  
Ultrasound Assisted ◽  
Hydrolysis Of ◽  
Mild Acid

The low molecular weight fraction of κ-carrageenan is useful in biomedical applications. An ultrasound-assisted acid hydrolysis of κ-carrageenan has been studied. κ-carrageenan with an initial number-average molecular weight of 629 kDa was dispersed in distilled water to form a 5 g/l solution. The pH (3 and 6) of the solution was adjusted by adding HCl solution. The depolymerization reaction was carried out in an ultrasonic device at various temperatures (30, 40, 50, and 60°C) and times (8, 16, 24, and 32 min). The experimental results showed that ultrasound positively contributed to acid hydrolysis process. The number-average molecular weight of the treated k-carrageenan was lower or the percentage of reduction was higher at lower pH, longer reaction time, and higher temperature. The lowest number-average molecular weight (14 kDa) or the highest percent of molecular weight reduction reduction (97.7%) was achieved after ultrasonic irradiation at 60°C and pH 3 for 32 min. Keywords: depolymerization; midpoint scission; ultrasonication

scholarly journals The usage of enzyme in ultrasound-assisted enzymatic extraction method and its effect on yield extract from Keji Beling (Strobilanthes crispus.) leaves

2018 ◽  
Vol 67 ◽  
pp. 03002
Author(s):  
Angelina ◽  
Mauhibah Yumna ◽  
Abdullah ◽  
Rita Arbianti ◽  
Tania Surya Utami ◽  
...  
Keyword(s):  
Extraction Method ◽  
Maximum Yield ◽  
Extraction Process ◽  
Enzymatic Extraction ◽  
Bioactive Substances ◽  
Green Extraction ◽  
Strobilanthes Crispus ◽  
Ultrasound Assisted ◽  
Effect On Yield ◽  
Hydrolysis Of

Green extraction process is a method which is used to obtain various plant extracts with minimum impact on the environment. Green extraction will reduce energy consumption, allow use of alternative solvents and renewable natural products, and ensure a safe and high quality extract. Ultrasound-assisted enzymatic extraction (UAEE) is one of green extraction method. UAEE is a mild, efficient and environmental friendly extraction method and it has been adopted for extracting various kinds of compounds. The use of enzyme will catalyze hydrolysis of the cytoderm and glycoproteins, therefore enhancing the release of bioactive substances by disrupting plant cells. The optimum extraction conditions with a maximum yield extract of 48.63% are as follows: the concentration of ethanol is 50% and the amount of added enzyme is 70 mg/g. Crude extract from keji beling leaves is tested using Gas Chromatography and Mass Spectrometry (GC-MS) to identify components that have anti-hypercholesterolemic activity, which are hexadecanoic acid, octadecanoic acid and demethyl squalene.

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