scholarly journals The usage of enzyme in ultrasound-assisted enzymatic extraction method and its effect on yield extract from Keji Beling (Strobilanthes crispus.) leaves

2018 ◽  
Vol 67 ◽  
pp. 03002
Author(s):  
Angelina ◽  
Mauhibah Yumna ◽  
Abdullah ◽  
Rita Arbianti ◽  
Tania Surya Utami ◽  
...  
Keyword(s):  
Extraction Method ◽  
Maximum Yield ◽  
Extraction Process ◽  
Enzymatic Extraction ◽  
Bioactive Substances ◽  
Green Extraction ◽  
Strobilanthes Crispus ◽  
Ultrasound Assisted ◽  
Effect On Yield ◽  
Hydrolysis Of

Green extraction process is a method which is used to obtain various plant extracts with minimum impact on the environment. Green extraction will reduce energy consumption, allow use of alternative solvents and renewable natural products, and ensure a safe and high quality extract. Ultrasound-assisted enzymatic extraction (UAEE) is one of green extraction method. UAEE is a mild, efficient and environmental friendly extraction method and it has been adopted for extracting various kinds of compounds. The use of enzyme will catalyze hydrolysis of the cytoderm and glycoproteins, therefore enhancing the release of bioactive substances by disrupting plant cells. The optimum extraction conditions with a maximum yield extract of 48.63% are as follows: the concentration of ethanol is 50% and the amount of added enzyme is 70 mg/g. Crude extract from keji beling leaves is tested using Gas Chromatography and Mass Spectrometry (GC-MS) to identify components that have anti-hypercholesterolemic activity, which are hexadecanoic acid, octadecanoic acid and demethyl squalene.

scholarly journals A green extraction design for enhancing flavonoid compounds from Ixora javanica flowers using a deep eutectic solvent

2020 ◽  
Vol 7 (10) ◽  
pp. 201116
Author(s):  
Nina Dewi Oktaviyanti ◽  
Kartini Kartini ◽  
Mochammad Arbi Hadiyat ◽  
Ellen Rachmawati ◽  
Andre Chandra Wijaya ◽  
...  
Keyword(s):  
Extraction Method ◽  
Choline Chloride ◽  
Deep Eutectic Solvent ◽  
Extraction Process ◽  
Raw Material ◽  
Molar Ratio ◽  
Flavonoid Compound ◽  
Total Flavonoids ◽  
Green Extraction ◽  
Optimum Extraction

In this study, an environmentally friendly extraction method for flavonoid compound from Ixora javanica , as a new raw material candidate for herbal medicine and cosmetics, was developed. The objectives of the present work were to provide recommendations for the optimal extraction conditions and to investigate the effects of any extraction parameters on flavonoid yields from the I. javanica flower. The extraction process was performed using deep eutectic solvent (DES) (choline chloride and propylene glycol at molar ratio of 1 : 1) and the ultrasound-assisted extraction method. Both single-factor and response surface analyses using three-level and three-factor Box Behnken designs were conducted to obtain the optimum flavonoid concentrations. The results showed that the optimum extraction conditions for total flavonoids featured an extraction time of 40 min, 25% water content in DES and a solid-to-liquid ratio of 1 : 25 g ml −1 . An extract obtained under optimum extraction conditions showed higher total flavonoid yields than an ethanolic extract which was used for comparison. Scanning electron microscope images demonstrated that both of the solvents also showed different effects on the outer surface of the I. javanica flower during the extraction process. In summary, our work succeeded in determining the optimum conditions for total flavonoids in the I. javanica flower using a green extraction method.

scholarly journals Optimization of Ultrasonic-Assisted Enzymatic Extraction of Tannic Acid from Chromolaena Odorota sp.

2021 ◽  
Vol 2129 (1) ◽  
pp. 012081
Author(s):  
AAA Kamal ◽  
M Mohamad ◽  
KA Sulaiman ◽  
NA Mohidem ◽  
NF Shoparwe ◽  
...  
Keyword(s):  
Tannic Acid ◽  
Duty Cycle ◽  
Extraction Method ◽  
Extraction Process ◽  
Enzyme Concentration ◽  
Extraction Time ◽  
Sonication Time ◽  
Chromolaena Odorata ◽  
Enzymatic Extraction ◽  
Ultrasonic Assisted

Abstract Previous study only implemented the time consuming and low amount of yield technique for extraction from Chromolaena odorata which is conventional method. Nonconventional extraction method with short extraction time and high amount of yield was applied in this study by applying ultrasound-assisted enzymatic extraction (UAEE). UAEE was used to extract tannic acid from Chromolaena odorata. The extraction parameters involved were enzyme concentration, sonication time and duty cycle at constant temperature of 50°C, solid to liquid ratio of 1:10 and sonication power at 60% amplitude. The optimum extraction process was found at cellulase enzyme concentration of 4%, sonication time of 60 minutes and duty cycle of 50% with the obtained concentration of tannic acid at 1.6152 mg/mL. The study showed that the UAEE could be employed to enhance yield of tannic acid, reduce the extraction time and ensuring green extraction method were applied in the study.

Ultrasound-Assisted Enzymatic Extraction Method for Multi-element Analysis of Rice

2020 ◽  
Vol 13 (8) ◽  
pp. 1549-1555
Author(s):  
Yaobaixue Qu ◽  
Zhao-Guang Yang ◽  
Haipu Li ◽  
Jie Yao ◽  
Bo Qiu
Keyword(s):  
Extraction Method ◽  
Enzymatic Extraction ◽  
Element Analysis ◽  
Ultrasound Assisted ◽  
Multi Element Analysis

scholarly journals EKSTRAKSI 1,8-CINEOLE DARI MINYAK DAUN EUCALYPTUS UROPHYLLA DENGAN METODE SOXHLETASI

2015 ◽  
Vol 4 (3) ◽  
pp. 52-57
Author(s):  
Irvan ◽  
Putra B. Manday ◽  
Januar Sasmitra
Keyword(s):  
Content Analysis ◽  
Essential Oil ◽  
Essential Oils ◽  
Filter Paper ◽  
Extraction Method ◽  
Maximum Yield ◽  
Soxhlet Extraction ◽  
Extraction Process ◽  
Pulp And Paper ◽  
Eucalyptus Urophylla

Eucalyptus urophylla plants (family: Myrtaceae, order: Myrtales ) usually are used only wooden parts for the manufacture of frames, pulp and paper, whereas their leaves can be used to produce essential oils in the pharmaceutical usage. The aim of this research is to extract the essential oils from leaves of Eucalyptus urophylla with soxhlet extraction method, in order to obtain maximum recovery of essential oils with good quality. Prior to the extraction process, the fresh leaves of Eucalyptus urophylla were reduced so that its size become 1 × 1 cm, and then dried in an oven at a temperature of 70 °C for 1 hour after being wrapped in a filter paper, after that followed by the extraction of the leaves using a variety of solvents, namely 96 % ethanol, n-hexane and dichloromethane, then proceed with the analysis of essential oil products (cineol content analysis). In this study, experiments were conducted with a variety of treatments such as the ratio of the volume of solvent to yield, the ratio of the mass of leaves of Eucalyptus urophylla to the yield on 5 cycles , and temperature of the solvent to yield. The conclusions of this research are the best extraction results with maximum cineol value (29.17 %) was obtained using dichloromethane solvent, while using 96 % ethanol produced the maximum yield of extract.

scholarly journals Optimization of Ultrasound-Assisted Extraction of Phloretin and Other Phenolic Compounds from Apple Tree Leaves (Malus domestica Borkh.) and Comparison of Different Cultivars from Estonia

Antioxidants ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 189
Author(s):  
Sana Ben-Othman ◽  
Hedi Kaldmäe ◽  
Reelika Rätsep ◽  
Uko Bleive ◽  
Alar Aluvee ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Apple Tree ◽  
Maximum Yield ◽  
Plant Secondary Metabolites ◽  
Polyphenolic Compounds ◽  
Tree Leaves ◽  
Ultrasound Assisted Extraction ◽  
Assisted Extraction ◽  
Ultrasound Assisted ◽  
Promising Source

Polyphenolic compounds, plant secondary metabolites essential for plant survival, are known for their high antioxidant and anti-inflammatory activity. In addition, several polyphenols, such as phloretin, also have potential antiviral effects, making these compounds potential ingredients of biofunctional foods. A promising source for the extraction of phloretin is a by-product of apple production—apple tree leaves. Focusing on green technologies, the first aim of the present study was to optimize the direct ultrasound-assisted extraction conditions to gain the maximum yield of phloretin from air-dried apple leaves. For the optimization of process parameters, we applied the response surface method with Box–Behnken design. The optimal extraction conditions were extraction time 14.4 min, sonication amplitude 10% and 10 g of sample per 100 mL solvent (70% ethanol, w/w). Using these conditions, we assessed the content of individual and total polyphenolic compounds along with antioxidant activity in the leaves of different autumn and winter apple cultivars grown in Estonia. The analyses were carried out with chromatographic (HPLC-DAD-MS/MS) and spectrophotometric methods. The phloretin concentration ranged from 292 to 726 µg/g and antioxidant activity from 6.06 to 11.42 mg GA eq./g, these being the highest in the local winter cultivars ‘Paide taliõun’ and ‘Tellissaare’, respectively.

scholarly journals Enzymatic Extraction and Characterization of Pectin from Cocoa Pod Husks (Theobroma cacao L.) Using Celluclast® 1.5 L

Molecules ◽  
2021 ◽  
Vol 26 (5) ◽  
pp. 1473
Author(s):  
Licelander Hennessey-Ramos ◽  
Walter Murillo-Arango ◽  
Juliana Vasco-Correa ◽  
Isabel Cristina Paz Astudillo
Keyword(s):  
Acid Content ◽  
Galacturonic Acid ◽  
Extraction Process ◽  
Chemical Extraction ◽  
Enzymatic Extraction ◽  
Acid Yield ◽  
Physicochemical Features ◽  
Enzyme Dosage ◽  
Cocoa Pod Husks

Cocoa pod husks are a waste generated during the processing of cocoa beans. We aimed to explore the enzymatic extraction of pectin using cellulases. The extraction process was optimized using a central composite design (CCD) and analyzed by response surface methodology (RSM). The parameters optimized were feedstock concentration (%), enzyme dosage (µL/g), and time (h). Three dependent variables were studied: pectin yield (g/100 g dry husk) (R2 = 97.02), galacturonic acid content (g/100 g pectin) (R2 = 96.90), and galacturonic acid yield (g/100 g feedstock) (R2 = 95.35). The optimal parameters were 6.0% feedstock concentration, 40 µL g−1 of enzyme, and 18.54 h, conditions that produced experimentally a pectin yield of 10.20 g/100 g feedstock, 52.06 g galacturonic acid/100 g pectin, and a yield 5.31 g galacturonic acid/100 g feedstock. Using the chemical extraction method, a yield of 8.08 g pectin/100 g feedstock and a galacturonic acid content of 60.97 g/100 g pectin were obtained. Using assisted sonication, a pectin yield of 8.28 g/100 g feedstock and a galacturonic acid content of 42.77 g/100 g pectin were obtained. Enzymatically optimized pectin has rheological and physicochemical features typical of this biomaterial, which provides an interesting alternative for the valorization of cocoa husks.

Microwave Assisted Multi-Stage Countercurrent Extraction of Dihydromyricetin from Ampelopsis grossedentataa

2011 ◽  
Vol 7 (4) ◽  
Author(s):  
Wei Li ◽  
Cheng Zheng ◽  
Jian Zhao ◽  
Zhengxiang Ning
Keyword(s):  
Extraction Efficiency ◽  
Extraction Process ◽  
Extraction Time ◽  
Countercurrent Extraction ◽  
Bioactive Substances ◽  
Extraction Solvent ◽  
Microwave Assisted ◽  
Multi Stage ◽  
The Matrix ◽  
Substantial Concentration

A novel microwave assisted multi-stage countercurrent extraction (MAMCE) technique was developed for the extraction of dihydromyricetin from Chinese rattan tea, Ampelopsis grossedentata. The technique combined the advantages of microwave heating and dynamic multi-stage countercurrent extraction and achieved marked improvement in extraction efficiency over microwave assisted batch extraction. Analysis of dihydromyricetin concentrations in the solvent and matrix throughout the extraction process showed that by dividing the extraction into multiple stages and exchanging of solvents between stages, steady and substantial concentration gradients were established between the matrix and solvent, thus enabling the achievement of high extraction efficiency. The yield of dihydromyricetin was significantly affected by temperature, pH, solvent/material ratio and extraction time, and optimal extraction conditions were found to be 80-100°C, at acidic pH with a solvent/material ratio of 25-30 to 1 and extraction time of 5-10 min. With the high extraction efficiency and low usage of extraction solvent, MAMCE could prove to be a promising extraction technique which can be applied to the extraction of dihydromyricentin and other bioactive substances from natural materials.

scholarly journals Integrated Metabolomics and Transcriptomics Using an Optimised Dual Extraction Process to Study Human Brain Cancer Cells and Tissues

Metabolites ◽  
2021 ◽  
Vol 11 (4) ◽  
pp. 240
Author(s):  
Alison Woodward ◽  
Alina Pandele ◽  
Salah Abdelrazig ◽  
Catherine A. Ortori ◽  
Iqbal Khan ◽  
...  
Keyword(s):  
Metabolic Pathways ◽  
Extraction Method ◽  
Limit Of Detection ◽  
Rna Extraction ◽  
Extraction Process ◽  
Extraction Methods ◽  
Serum Starvation ◽  
Extraction Techniques ◽  
Metabolic Genes ◽  
Dual Extraction

The integration of untargeted metabolomics and transcriptomics from the same population of cells or tissue enhances the confidence in the identified metabolic pathways and understanding of the enzyme–metabolite relationship. Here, we optimised a simultaneous extraction method of metabolites/lipids and RNA from ependymoma cells (BXD-1425). Relative to established RNA (mirVana kit) or metabolite (sequential solvent addition and shaking) single extraction methods, four dual-extraction techniques were evaluated and compared (methanol:water:chloroform ratios): cryomill/mirVana (1:1:2); cryomill-wash/Econospin (5:1:2); rotation/phenol-chloroform (9:10:1); Sequential/mirVana (1:1:3). All methods extracted the same metabolites, yet rotation/phenol-chloroform did not extract lipids. Cryomill/mirVana and sequential/mirVana recovered the highest amounts of RNA, at 70 and 68% of that recovered with mirVana kit alone. sequential/mirVana, involving RNA extraction from the interphase of our established sequential solvent addition and shaking metabolomics-lipidomics extraction method, was the most efficient approach overall. Sequential/mirVana was applied to study a) the biological effect caused by acute serum starvation in BXD-1425 cells and b) primary ependymoma tumour tissue. We found (a) 64 differentially abundant metabolites and 28 differentially expressed metabolic genes, discovering four gene-metabolite interactions, and (b) all metabolites and 62% lipids were above the limit of detection, and RNA yield was sufficient for transcriptomics, in just 10 mg of tissue.

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