Green and ecofriendly biosynthesis of selenium nanoparticles using Urtica dioica (Stinging nettle) leaf extract: Antimicrobial and anticancer activity

2021 ◽  
pp. 2100432
Author(s):  
Amr H. Hashem ◽  
Salem S. Salem
Keyword(s):  
Anticancer Activity ◽  
Leaf Extract ◽  
Urtica Dioica ◽  
Selenium Nanoparticles ◽  
Stinging Nettle

Green synthesis and characterization of zero-valent iron nanoparticles using stinging nettle (Urtica dioica) leaf extract

2017 ◽  
Vol 6 (5) ◽  
Author(s):  
Alireza Ebrahiminezhad ◽  
Alireza Zare-Hoseinabadi ◽  
Aydin Berenjian ◽  
Younes Ghasemi
Keyword(s):  
Leaf Extract ◽  
Iron Nanoparticles ◽  
Urtica Dioica ◽  
Zero Valent Iron ◽  
Synthesis And Characterization ◽  
Stinging Nettle ◽  
Aqueous Leaf Extract ◽  
Zero Valent Iron Nanoparticles ◽  
First Time

AbstractFor the first time, aqueous leaf extract of

Bis (Isothiocyanatomethyl) Benzene, A Plant Derived Anti-Neoplastic Compound: Purified from Moringa Oleifera Leaf Extract

2019 ◽  
Vol 19 (5) ◽  
pp. 677-686 ◽  
Author(s):  
Samrat Paul ◽  
Piyali Basak ◽  
Namrata Maity ◽  
Chayan Guha ◽  
Nandan Kumar Jana
Keyword(s):  
Anticancer Activity ◽  
Leaf Extract ◽  
Moringa Oleifera ◽  
Folk Medicine ◽  
Drug Formulation ◽  
Indigenous Species ◽  
Cancer Cell Growth ◽  
Apoptotic Pathway ◽  
Dimeric Form ◽  
Mcf 7

Background: Moringa oleifera lam, commonly known as “Sajina”, is an indigenous species to India. In our folk medicine, it is used for the treatment of Canker (cancer). The Moringa oleifera leaf extract contains many phyto-compounds, with some being anti-neoplastic in nature. Objective: Our preliminary study showed that the leaf extract significantly kills cancer cells compared to normal cells. On searching for the new phyto-compound, Bis-isothiocyanatomethyl) benzene was purified and isolated. Methods: The sequential process of fractional distillation, column chromatography, followed by TLC and HPLC is performed for purification. Every fraction from each step was tested on HeLa cell line for evaluating the presence of the phyto-compound. Results and Conclusion: FTIR peak analysis of a single phyto-compound shows the presence of thiocyanate group, aromatic carbon group. 1H & 13C NMR peak analysis along with High-resolution mass spectroscopy (HRMS) calculation confirm the chemical structure with IUPAC name [Bis (Isothiocyanatomethyl) benzene]. Previously, Isothiocyanatomethyl- benzene solely or in conjugation with sugar molecule has been reported, but its dimeric form in nature hasnot yet been published anywhere. It shows anticancer activity by retarding cancer cell growth & inhibits carcinogenesis on HeLa, MCF-7, and MDA-MB-231 cell lines by caspase 3 apoptotic pathway and showed comparatively less cytotoxicity to PBMC cell. It shows anticancer activity almost the same as the market available drug Cis-Platin. Therefore, further extrapolating its activity with different concentrations may result in its use as a drug formulation for the treatment of cancer.

Enhanced Anticancer Activity and Apoptosis Effect of Bioactive Compound Quercetin Extracted from Ocimum sanctum Leaves

2020 ◽  
Vol 10 ◽  
Author(s):  
Amutha Santhanam ◽  
Naveen Kumar Chandrasekharan ◽  
Rajangam Ilangovan
Keyword(s):  
Cell Cycle ◽  
Anticancer Activity ◽  
Cancer Cells ◽  
Leaf Extract ◽  
Nickel Nanoparticles ◽  
Bioactive Compound ◽  
Cell Cycle Analysis ◽  
Ocimum Sanctum ◽  
Sem Images ◽  
Ft Ir

Background: The occurrence of Cancer results in cellular changes that causes the uncontrolled growth and division of cells. Apoptosis removes cells during development and eliminates the potentially cancerous cells. The bioactive compounds present in the herbal plant shows cytotoxic activity that result in apoptosis. The traditional herbal plants are used world-wide both in allopathy and other traditional ways. Objective: The main objective of this study is to extract the bioactive compound Quercetin from the medicinally significant plant Ocimum sanctum and also to develop nanomedicine as Qu-PEG-NiGs. Materials and Methods: Leaf extract of the medicinally significant plant Ocimum sanctum (O. sanctum) has been used for the synthesis of nickel nanoparticles (NiGs) and extraction of quercetin (Qu). The ethanolic extract of Ocimum sanctum is added to 1 mM Nickel Nitrate (Ni(NO3)2) and stirred for 3 hrs at RT and dried at 60°C for 3hrs and calcinated at 400°C for 2hrs and characterized using Uv-Vis Spectrophotometer, FT-IR, SEM, DLS and Zeta potential. The Quercetin is isolated from Ocimum sanctum leaf extract using the reflux condenser method. The bio-polymer is being PEG-coated over NiGs and Quercetin is loaded into it. The apoptosis activity using MCF-7 cells is performed with Qu-PEG-NiGs. The purity of Quercetin is characterized using HPLC. In order to analyse apoptosis efficiency, MTT assay, Reactive Oxygen Species (ROS), Cell cycle analysis has been performed. Results: The NiGs absorption spectrum gives a peak at 408nm. The FT-IR confirms the presence of particular functional groups shifting from the compound NiGs and then coated with PEG-Qu-NiGs. The SEM images show the size of NiGs ranging from 27.3 nm to 40.4 nm with varied morphology such as hexagonal and other irregular shapes. The presence of Quercetin extracted from the leaf powder is approximately 1.5 mg/g. The ROS results show the Qu-PEG-NiGs induced efficiency of the apoptosis, while the increased concentrations promote ROS and lead to activation of the apoptosis. The cell cycle analysis has shown the cytotoxic effect. Conclusion: PEG-coated nickel nanoparticles can be used as a promising chemotherapeutic agent against MCF7 breast cancer cells. It is the evidence to further studies for evaluating Qu-PEG-NiGs anticancer activity on different types of cancer cells.

scholarly journals An unusual lectin from stinging nettle (Urtica dioica ) rhizomes

FEBS Letters ◽  
1984 ◽  
Vol 177 (1) ◽  
pp. 99-103 ◽  
Author(s):  
Willy J. Peumans ◽  
Marc De Ley ◽  
Willem F. Broekaert
Keyword(s):  
Urtica Dioica ◽  
Stinging Nettle

scholarly journals Urtica dioica (Stinging Nettle) – Properties, Uses and Applications

2021 ◽  
pp. 154-159
Author(s):  
Anjali, Sushma ◽  
Reena Sharma
Keyword(s):  
Fungal Infections ◽  
Database Search ◽  
Urtica Dioica ◽  
Pharmacological Properties ◽  
Electronic Database ◽  
Stinging Nettle ◽  
Wide Range ◽  
The World ◽  
Electronic Database Search ◽  
Pharmacological Potential

Utricaceae is a family of herbs and shrubs that can be found in a variety of habitats around the world. A lot of research has been carried out till date targeted for close understanding of this medicinal plant. The botanical distribution, Ethnomedicinal applications, Traditional uses as well as Pharmacological properties of the Urtica genus, are discussed in this study. The composition such as flavonoids and array of phenolic compounds which includes alcohols, Diocanol, Diol glucosides, Terpenes diols, and sugars as well are an inclusion in the genus Urtica. A wide range of research reports have been published representing its biological and pharmacological potential against cancer, tumors, bacterial, viral or fungal infections significantly. The information about the Urtica genus has been extracted using electronic database search such as Google Scholar and Pubmed as well as a library search for peer-reviewed journal publications.

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