Hepatoprotective Effect of Leaves of Morinda tinctoria Roxb. Against Non-alcoholic Fatty Liver Disease in Rats

Aim: To study the hepatoprotective and antioxidant activity of 50% aqueous-alcoholic leaves extract of Morinda tinctoria (Rubiaceae) against non-alcoholic fatty liver disease. Study Design: All experiments involving animals complies with the ethical standards of animal handling and approved by institutional animal ethical and welfare committee of the Institute of Pharmacy, PSIT (1273/AC/09/ CPCSEA) and plant were collected from Ranan Nagar, Madurai, Tamil Nadu, India and was authenticated by Dr. Navin K. Ambasht, Head and Associate Professor, Botany Department, Christ Church College, Kanpur, Uttar Pradesh, India. Place and Duration of Study: The study was carried out at Institute of Pharmacy, PSIT, Kanpur, Uttar Pradesh, India, during 2018-21. Methodology: The hepatoprotective potential of Morinda tinctoria leaves extract (MTLE) 150 and 300 mg/kg body weight was studied on Methionine and Choline deficient diet, High Fat Diet, Cholesterol and Cholate diet, and Streptozotocin + HFD induced non-alcoholic fatty liver disease. At the end of the treatment blood sample was collected from direct cardiac puncture and analysed for various parameter like alanine aminotransferase, aspartate transaminase, low density lipoprotein, high density lipoprotein, triglycerides, total cholesterol, free fatty acid and malondialdehyde. Results: The phytochemical investigation of extract showed presence of alkaloids, flavonoids, amino acid, saponin, tannins, phenols, carbohydrate and for the first time the present study showed that Morinda tinctoria leaves extract reduced level of alanine aminotransferase, aspartate transaminase, triglycerides, total cholesterol, low density lipoprotein, free fatty acid, malondialdehyde and enhance the level of Superoxide dismutase, High Density Lipoprotein and it also returned hepatic damage towards normal which further supports hepatoprotective and antioxidant activity of M. tinctoria. leaves extracts. Conclusions: M. tinctoria leaves extract showed maximum curation in the dose 300 mg/kg body weight against non-alcoholic fatty liver disease.

Green Synthesis of Copper Oxide Nanoparticles Mediated by Aqueous Leaf Extracts of Leucas aspera and Morinda tinctoria

Copper oxide nanoparticles were successfully synthesized using the aqueous leaf extracts of Leucas aspera and Morinda tinctoria plant material with copper sulfate (CuSO4.5H2O) as the precursor. The crystalline nature and morphology of the synthesized sample were identified using XRD and SEM analytical instrumentation and found that the crystal was in the monoclinic phase, and the average particle size was estimated as 30.32nm and 18.72nm for both the samples. The functional groups were identified using FTIR spectroscopy, and the strong absorption peak at 620 cm-1 and 615cm-1 confirms the presence of Cu-O vibration. The optical bandgap of the plant leaf extract mediated CuO particles was calculated based on the results obtained from UV-Vis spectroscopy and found that the value of the energy gap is 5.6eV and 3.16eV. The antibacterial activity of plant samples was carried out by the disc diffusion method. The test compounds' concentrations were taken in DMSO and used in the concentration of 500 µg and 1000 µg /disc. The zone of inhibition formed by the prepared CuO nanoparticles was good and compared with Amikacin's standard value. The study reports the plant leaf extract mediated CuO nanoparticles might find suitable application in the field of nanotechnology.

Phytochemical and pharmacological properties of Morinda tinctoria – A review

Indian systems of medicine and alternative medical systems were very successful with the use and incorporation of herbs to treat diseases. The scientific evidence was not established in the traditional claims of the herbs. In this review extensive segregation of the research work that had been performed on the plant Morinda tinctoria is done. The Pharmacological activities, Phytochemical work and pharmacogostical work that was performed on the plant had been reviewed and documented in this paper.

EVALUATION OF BIOACTIVITIES OF MORINDA TINCTORIA LEAVES EXTRACT FOR PHARMACOLOGICAL APPLICATIONS.

Objective: The present study was aimed to prepare Morinda tinctoria leaves extracts with the different solvent system and to evaluate the bioactivities.Methods: The extracts of M. tinctoria were qualitatively analyzed for the primary phytochemical content. The functional groups of extract were determined by Fourier-transform infrared spectroscopy (FT-IR) analysis. The antimicrobial properties were determined by plate assays. The antioxidant and in vitro anti-inflammatory properties and membrane stabilizing nature of aqueous extract of M. tinctoria (AEM) were measured using a spectrophotometer.Results: The aqueous, ethanolic, and acetone extracts of M. tinctoria were prepared. AEM contains quinones, steroids, terpenoids, phenols, glycosides, and tannins. FTIR result showed that AEM comprises of alkyl halides, 1°, 2° amines, aromatics, aliphatic amines, alcohols, carboxylic acids, esters, ethers, and alkanes, saturated aliphatic, and phenolic groups. The antimicrobial property of M. tinctoria varied based on the solvent used for the extraction. About 86.90±0.36, 78.58±0.13, and 80.33±0.09% of total antioxidant capacity, reducing power, and hydrogen peroxide scavenging activity were observed in AEM, respectively. The 1, 1- diphenyl 2-picrylhyorazyl and 2, 2-Azinobis-(3 ethylbenzothiazoline-6-sulfonic acids) assay results indicated 85.20±0.50 and 52.41±0.60% of free radical scavenging activity in AEM. The protease activity (44.10±0.26%) and protein degradation (44.38±0.58%) were proscribed by AEM. AEM prevents 69.36±0.20% of cell lysis.Conclusion: The results revealed that the AEM leaves were harmless and enriched with potent bioactive principles, which is further used for food and pharmacological applications.