First Extensive Polyphenolic Profile of Erodium cicutarium with Novel Insights to Elemental Composition and Antioxidant Activity

SummaryThe beneficial effects of walnuts and walnut products on human health, due primarily to their rich polyphenolic content, have been appreciated as an empirical fact for centuries. The purpose of this study is to determine the polyphenolic contents of liqueurs made from the walnut selections ‘Rasna’ and ‘Sava’ and the walnut cultivar ‘Šampion’ (all harvested at three different times), as well as the polyphenolic contents of their green husks. The walnut liqueurs were prepared according to two traditional recipes using young walnut fruits. The highest antioxidant capacity was recorded in the liqueur made from the ‘Rasna’ walnut selection (89.94%), whereas the highest contents of phenols (83.28 mg GAE/g FW), flavonoids (0.83 mg QE/g FW) and proanthocyanidins were found in the liqueur made from the ‘Šampion’ cultivar (14.75 mg CE/g FW). The youngest ‘Rasna’ walnuts, harvested at the first experimental time point, exhibited the highest phenolic and tannin contents, whereas the highest flavonoid content was observed in the ‘Šampion’ cultivar. The biochemical results obtained indicate a decrease in the polyphenolic content of walnut fruits with their growth and development. Moreover, the polyphenolic profiles of the walnut liqueurs considered were found to be greatly affected by the method of preparation and the cultivar/selection of walnuts.

Download Full-text

Polyphenolic profile and antioxidant activity of olive mill wastewater from two Sicilian olive cultivars: Cerasuola and Nocellara etnea

European Food Research and Technology ◽

10.1007/s00217-017-2893-3 ◽

2017 ◽

Vol 243 (11) ◽

pp. 1895-1903 ◽

Cited By ~ 12

Author(s):

Maria Domenica Di Mauro ◽

Roberta Carla Giardina ◽

Giovanni Fava ◽

Emanuele Francesco Mirabella ◽

Rosaria Acquaviva ◽

...

Keyword(s):

Antioxidant Activity ◽

Olive Mill Wastewater ◽

Olive Cultivars ◽

Polyphenolic Profile ◽

Olive Mill

Download Full-text

Polyphenolic Composition and Antioxidant Activity of Uncaria tomentosa Commercial Bark Products

Antioxidants ◽

10.3390/antiox8090339 ◽

2019 ◽

Vol 8 (9) ◽

pp. 339 ◽

Cited By ~ 6

Author(s):

Mirtha Navarro ◽

Elizabeth Arnaez ◽

Ileana Moreira ◽

Alonso Hurtado ◽

Daniela Monge ◽

...

Keyword(s):

Antioxidant Activity ◽

Costa Rica ◽

Biological Activities ◽

Pearson Correlation ◽

Commercial Products ◽

Uncaria Tomentosa ◽

Average Value ◽

Polyphenolic Composition ◽

Significant Difference ◽

Polyphenolic Profile

Uncaria tomentosa, which is widely commercialized as an herbal medicine, constitutes an important source of secondary metabolites with diverse biological activities. For instance, we have previously reported, for the first time, of a polyphenolic profile rich in proanthocyanidins from extracts of U. tomentosa plants, as well as their antioxidant capacity, antimicrobial activity on aerial bacteria, and cytotoxicity on cancer cell lines. These promising results prompted this research to evaluate the polyphenolic contents of U. tomentosa commercial products. We report a detailed study on the polyphenolic composition of extracts from U. tomentosa bark products (n = 18) commercialized in Costa Rica and Spain. Using HPLC-DAD/TQ-ESI-MS, a total of 25 polyphenolic compounds were identified, including hydroxybenzoic and hydroxycinnamic acids, flavan-3-ol monomers, procyanidin dimers, procyanidin trimers, as well as propelargonidin dimers. Our findings on the polyphenolic profile for all commercial samples show analogous composition to previous reports on U. tomentosa bark material, for instance a 41–49% content of procyanidin dimers and the presence of propelargonidin dimers (8–15%). However, most of the 18 commercial samples exhibit low proanthocyanidin contents (254.8–602.8 µg/g), more similar to previous U. tomentosa inner bark reports, while some exhibit better results, with one sample (SP-2) showing the highest contents (2386.5 µg/g) representing twice the average value of all 18 commercial products. This sample also exhibits the highest total phenolics (TP) and total proanthocyanidins (PRO) contents, as well as the highest Oxygen Radical Absorbance Capacity (ORAC) value (1.31 µg TE/g). One-way Analysis of Variance (ANOVA) with a Tukey post hoc test indicated significant difference (p < 0.05) between products from Costa Rica and Spain for TP and PRO findings, with samples from Spain exhibiting a higher average value. In addition, Pearson correlation analysis results showed a positive correlation (p < 0.05) between TP, PRO, and ORAC results, and an especially important correlation between ORAC antioxidant values and procyanidin dimers (r = 0.843, p < 0.05), procyanidin trimers (r = 0.847, p < 0.05), and propelargonidin dimers (r = 0.851, p < 0.05) contents. Finally, Principal Component Analysis (PCA) results indicated some variability in the composition regardless of their origin. However, only one sample (SP-2) stands out significatively, showing the highest PC1 because of its particularly high proanthocyanidins contents, which could be attributed to the 15% bark polyphenolic extract labeled in this commercial product, which differentiate this sample from all other 17 commercial samples. Therefore, our findings confirmed previous results on the value of extracts in the elaboration of potential commercial products from U. tomentosa, rich in proanthocyanidins and exhibiting high antioxidant activity.

Download Full-text

Characterization of antioxidant activity, vitamins, and elemental composition of ciris (Asphodelus aestivus L.) from Tunceli, Turkey

Instrumentation Science & Technology ◽

10.1080/10739149.2016.1272463 ◽

2016 ◽

Vol 45 (5) ◽

pp. 469-478 ◽

Cited By ~ 1

Author(s):

Ilkay Unal ◽

Olcay Kaplan Ince

Keyword(s):

Antioxidant Activity ◽

Elemental Composition ◽

Asphodelus Aestivus

Download Full-text

Effect of Plasma Exposure Time on the Polyphenolic Profile and Antioxidant Activity of Fresh-Cut Apples

Applied Sciences ◽

10.3390/app8101939 ◽

2018 ◽

Vol 8 (10) ◽

pp. 1939 ◽

Cited By ~ 7

Author(s):

Silvia Tappi ◽

Ileana Ramazzina ◽

Federica Rizzi ◽

Giampiero Sacchetti ◽

Luigi Ragni ◽

...

Keyword(s):

Antioxidant Activity ◽

Exposure Time ◽

Metabolic Response ◽

Atmospheric Plasma ◽

Plasma Exposure ◽

Fresh Tissue ◽

Apple Slices ◽

Fresh Cut ◽

Polyphenolic Profile ◽

The Impact

Cold atmospheric plasma (CAP) has shown good potentiality for the decontamination and stabilization of fresh fruit and vegetable products; however, information about its effect on nutritional quality is still scarce. The aim of this research was to evaluate the impact of a form of indirect treatment known as Dielectric Barrier Discharge (DBD) on apple slices—more specifically, the polyphenolic profile and antioxidant activity of fresh-cut Pink Lady apples. Atmospheric plasma was generated using air as feed gas, and directed to apple slices for up to 30 min. The effect of plasma treatment on physico-chemical parameters was mainly observed as a slight acidification of the tissue and reduction of browning after an extended period of exposure. The samples’ phenolic profile was significantly affected after 10 min of treatment, both in quantitative (an approximately 20% increase) and qualitative terms, while with increasing exposure time a progressive decrease of all polyphenol classes was observed. The antioxidant activity, evaluated by different in-vitro methods, followed a similar trend, increasing after 10 min of processing and then decreasing. Results highlighted how plasma exposure promotes a metabolic response of the fresh tissue, and the importance of carefully controlling the exposure time in order to minimize the loss of nutritional properties.

Download Full-text

The Effect of Adding the Leaves and Fruits of Morus alba to Rape Honey on Its Antioxidant Properties, Polyphenolic Profile, and Amylase Activity

Molecules ◽

10.3390/molecules25010084 ◽

2019 ◽

Vol 25 (1) ◽

pp. 84 ◽

Cited By ~ 2

Author(s):

Monika Tomczyk ◽

Michał Miłek ◽

Ewelina Sidor ◽

Ireneusz Kapusta ◽

Wojciech Litwińczuk ◽

...

Keyword(s):

Antioxidant Activity ◽

Phenolic Content ◽

Antioxidant Properties ◽

Inhibitory Effect ◽

Flavonoid Glycosides ◽

Total Phenolic ◽

Antioxidant Compounds ◽

Mulberry Leaves ◽

Hydrolyzing Enzymes ◽

Polyphenolic Profile

Two various species of mulberry (Morus sp.) were selected to enrich rape honey with dried leaves or lyophilized fruits (4% w/v). Finally, fruits and leaves of the ‘Ukraińska’ clone were introduced into the honey during creaming in concentrations from 1 to 4% w/v. The total phenolic content, antioxidant activity, anthocyanins content, and polyphenolic profile were tested in plant extracts and enriched honeys. Moreover, α-glucosidase, β-galactosidase, and diastase activities were investigated in honeys. For mulberry extracts, chlorogenic acid isomers and rutin were considered main antioxidant compounds. The antioxidant activity of honey enriched with mulberry leaves increased even more than 50 times, due to introducing numerous phenolic acids and flavonoid glycosides. A significant decrease in the diastase activity in honey depending on the content of added mulberry leaves (almost 50% decrease in the case of 4% addition) was found, suggesting the inhibitory effect of honey with mulberry leaves against carbohydrate hydrolyzing enzymes.

Download Full-text

Polyphenolic Composition and In Vitro Antioxidant Activity of Red Grape Seeds as Byproducts of Short and Medium-Long Fermentative Macerations

Foods ◽

10.3390/foods9101451 ◽

2020 ◽

Vol 9 (10) ◽

pp. 1451 ◽

Cited By ~ 1

Author(s):

Antonella Bosso ◽

Claudio Cassino ◽

Silvia Motta ◽

Loretta Panero ◽

Christos Tsolakis ◽

...

Keyword(s):

Antioxidant Activity ◽

High Performance ◽

Grape Seeds ◽

Antioxidant Power ◽

Polyphenolic Composition ◽

Polyphenolic Content ◽

Red Grape ◽

Polyphenolic Profile ◽

In Vitro Antioxidant Activity

The polyphenolic composition and antioxidant activity of grape seeds, as byproducts of red winemaking, depend on various factors, such as grape cultivar, vintage effect, grape maturity and winemaking methods. In the present work, the influence of the maceration length on the polyphenolic and antioxidant characteristics of the seeds of four Italian red grape cultivars (‘Barbera’, ‘Grignolino’, ‘Nebbiolo’, and ‘Uvalino’), sampled from the fermentation tanks after short (two days) and medium-long (7–21 days) macerations, was studied with spectrophotometric methods, high-performance liquid chromatography (HPLC), and three different antioxidant assays (2,2′-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS), ferric reducing antioxidant power (FRAP) and 2,2 diphenyl-1-picrylhydrazyl (DPPH)). The total polyphenolic content (gallic acid equivalent (GAE)) of the seeds sampled after short macerations ranged between 24.5 and 60.1 mg/g dry weight (DW), and it dropped to 20.0–37.5 mg/g DW after medium-long macerations. The polyphenolic profile of the shortly macerated seeds was related to the varietal characteristics, while, after longer macerations, the influence of the maceration length prevailed on the cultivar effect. The multiple in vitro antioxidant activity tests (ABTS, FRAP and DPPH), although based on different mechanisms capable of highlighting behavioral differences between the different polyphenolic compounds, were highly correlated with each other and with the polyphenolic parameters; the qualitative differences between the matrices in the polyphenolic profile were probably less important than the quantitative differences in the polyphenolic content. The relations with the polyphenolic content were linear, except for the Efficient Concentration (EC20) parameter, whose relation was better described by a hyperbolic equation.

Download Full-text