Proliferation rate of stem cells derived from human dental pulp and identification of differentially expressed genes

2014 ◽  
Vol 38 (5) ◽  
pp. 582-590 ◽  
Author(s):  
Muhammad Fawwaz Abdullah ◽  
Siti Fadilah Abdullah ◽  
Nor Shamsuria Omar ◽  
Zuliani Mahmood ◽  
Siti Noor Fazliah Mohd Noor ◽  
...  
Keyword(s):  
Stem Cells ◽  
Differentially Expressed Genes ◽  
Dental Pulp ◽  
Proliferation Rate ◽  
Differentially Expressed ◽  
Human Dental Pulp

scholarly journals METTL3-mediated M6a Modification Regulates Cell Cycle Progression of Dental Pulp Stem Cells

2021 ◽  
Author(s):  
Haiyun Luo ◽  
Wenjing Liu ◽  
Yanli Zhang ◽  
Xiao Jiang ◽  
Shiqing Wu ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Tissue Engineering ◽  
Stem Cells ◽  
Flow Cytometry ◽  
Differentially Expressed Genes ◽  
Dental Pulp ◽  
Cell Cycle Control ◽  
Dental Pulp Stem Cells ◽  
Differentially Expressed ◽  
Expression Level

Abstract Background: Dental pulp stem cells (DPSCs) exhibited self-renewal, pluripotency capacity and served as promising cells source in endodontic regeneration and tissue engineering. Meanwhile, the regenerative capacity of DPSCs is limited and reduced in long lifespan. N6-methyladenosine (m6A) is the most prevalent, reversible internal modification in RNAs. The methyltransferases complex and demethylases mediated m6A methylation and cooperated to impact various biological processes associated with stem cell fate determination. However, the biological effect of m6A methylation in DPSCs remained unclear. Methods: Cell surface markers and differentiation potential of primary DPSCs were identified and m6A immunoprecipitation with deep sequencing (m6A RIP-seq) was used to uncover characteristics of m6A modifications in DPSCs transcriptome. Expression level of m6A-related genes were evaluated in immature/mature pulp tissues and cells. Lentiviral vectors were constructed to knockdown or overexpress methyltransferase like 3 (METTL3). Cell morphology, viability, senescence and apoptosis were further analyzed by β-galactosidase, TUNEL staining and flow cytometry. Bioinformatic analysis combing m6A RIP and shMETTL3 RNA-seq was used to functionally enrich overlapped genes and screen target of METTL3. Cell cycle distributions were assayed by flow cytometry and m6A RIP-qPCR was used to confirm METTL3 mediated m6A methylation in DPSCs. Results: Here, m6A peaks distribution, binding area and motif in DPSCs were first revealed by m6A RIP-seq. We also found a relative high expression level of METTL3 in immature DPSCs with superior regenerative potential and METTL3 knockdown induced cell apoptosis and senescence. Furthermore, Conjoint analysis of m6A RIP and RNA-sequencing showed differentially expressed genes affected by METTL3 depletion was mainly enriched in cell cycle, mitosis and alteration of METTL3 expression resulted in cell cycle arrest which indicated METTL3 make essential effect in cell cycle control. To further investigate underlying mechanisms, we explored proteins interaction network of differentially expressed genes and Polo-like Kinase 1 (PLK1), a critical cycle modulator was identified as target of METTL3-mediated m6A methylation in DPSCs. Conclusions: These results revealed m6A methylated hallmarks in DPSCs and a regulatory role of METTL3 in cell cycle control. Our study shed light on therapeutic approaches in vital pulp therapy and serve new insight in stem cells based tissue engineering.

scholarly journals THE EFFECT OF FETAL CALF SERUM ON HUMAN DENTAL PULP STEM CELLS

2013 ◽  
Vol 56 (4) ◽  
pp. 142-149 ◽  
Author(s):  
Jakub Suchánek ◽  
Tereza Suchánková Kleplová ◽  
Martin Kapitán ◽  
Tomáš Soukup
Keyword(s):  
Stem Cells ◽  
Side Effects ◽  
Chromosomal Aberrations ◽  
Dental Pulp ◽  
Fetal Calf Serum ◽  
Calf Serum ◽  
Dental Pulp Stem Cells ◽  
Proliferation Rate ◽  
Human Dental Pulp

Aims: Authors studied potential side effects of fetal calf serum (FCS) in cultivation media on human dental pulp stem cells (DPSC) during long term cultivation. Methods: Two lines of DPSC obtained healthy donors (male 22 years, female 23 years) were used. Both lines were cultivated under standard cultivation conditions in four different media containing 10% or 2% FCS and substituted with growth factors. During long term cultivation proliferation ability, karyotype and phenotype of DPSC were measured. Results: Both lines of DPSC cultivated in a media containing 2% FCS and ITS supplement showed the highest number of population doublings. On the other hand the proliferation rate of DPSC cultivated in a media with 2% FCS without ITS supplement was slowest. Proliferation rate of DPSC cultivated in 10% FCS media with or without FGF-2 was comparable. DPSC cultivated in a media with 10% FCS showed a significantly higher amount of chromosomal aberrations. These chromosomal aberrations do not seem to be clonal but surprisingly we found large amounts of tetraploid cells in the 9th passage in both media containing 10% FCS. Conclusions: Our study proved that cultivation of DPSC in media containing higher concentration of FCS has critical side effects on cell chromosomal stability.

scholarly journals Modulation of Cell Death and Promotion of Chondrogenic Differentiation by Fas/FasL in Human Dental Pulp Stem Cells (hDPSCs)

2020 ◽  
Vol 8 ◽  
Author(s):  
Alessandra Pisciotta ◽  
Giulia Bertani ◽  
Laura Bertoni ◽  
Rosanna Di Tinco ◽  
Sara De Biasi ◽  
...  
Keyword(s):  
Stem Cells ◽  
Cell Death ◽  
Dental Pulp ◽  
Chondrogenic Differentiation ◽  
Dental Pulp Stem Cells ◽  
Human Dental Pulp

Chrysin induces osteogenic differentiation of human dental pulp stem cells

2021 ◽  
Vol 400 (2) ◽  
pp. 112466
Author(s):  
J.F. Huo ◽  
M.L. Zhang ◽  
X.X. Wang ◽  
D.H. Zou
Keyword(s):  
Stem Cells ◽  
Osteogenic Differentiation ◽  
Dental Pulp ◽  
Dental Pulp Stem Cells ◽  
Human Dental Pulp

scholarly journals Human Dental Pulp Stem Cells (DPSCs) Therapy in Rescuing Photoreceptors and Establishing a Sodium Iodate-Induced Retinal Degeneration Rat Model

2021 ◽  
Author(s):  
Chenshen Lam ◽  
Hiba Amer Alsaeedi ◽  
Avin Ee-Hwan Koh ◽  
Mohd Hairul Nizam Harun ◽  
Angela Ng Min Hwei ◽  
...  
Keyword(s):  
Stem Cells ◽  
Retinal Degeneration ◽  
Rat Model ◽  
Dental Pulp ◽  
Dental Pulp Stem Cells ◽  
Sodium Iodate ◽  
Human Dental Pulp

scholarly journals Retinoic and ascorbic acids induce osteoblast differentiation from human dental pulp mesenchymal stem cells

2021 ◽  
Vol 11 (2) ◽  
pp. 143-148
Author(s):  
Lina M. Escobar ◽  
José Daniel Escobar ◽  
Zita Bendahan ◽  
Jaime E. Castellanos
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Dental Pulp ◽  
Osteoblast Differentiation ◽  
Human Dental Pulp ◽  
Ascorbic Acids

scholarly journals Methylglyoxal-Dependent Glycative Stress Is Prevented by the Natural Antioxidant Oleuropein in Human Dental Pulp Stem Cells through Nrf2/Glo1 Pathway

Antioxidants ◽  
2021 ◽  
Vol 10 (5) ◽  
pp. 716
Author(s):  
Simona Delle Delle Monache ◽  
Fanny Pulcini ◽  
Roberta Frosini ◽  
Vincenzo Mattei ◽  
Vincenzo Nicola Talesa ◽  
...  
Keyword(s):  
Stem Cells ◽  
Dental Pulp ◽  
Dental Pulp Stem Cells ◽  
Great Promise ◽  
Oral Diseases ◽  
Bioactive Component ◽  
Glycation End Products ◽  
Abnormal Accumulation ◽  
Synthetic Agents ◽  
Human Dental Pulp

Methylglyoxal (MG) is a potent precursor of glycative stress (abnormal accumulation of advanced glycation end products, AGEs), a relevant condition underpinning the etiology of several diseases, including those of the oral cave. At present, synthetic agents able to trap MG are known; however, they have never been approved for clinical use because of their severe side effects. Hence, the search of bioactive natural scavengers remains a sector of strong research interest. Here, we investigated whether and how oleuropein (OP), the major bioactive component of olive leaf, was able to prevent MG-dependent glycative stress in human dental pulp stem cells (DPSCs). The cells were exposed to OP at 50 µM for 24 h prior to the administration of MG at 300 µM for additional 24 h. We found that OP prevented MG-induced glycative stress and DPSCs impairment by restoring the activity of Glyoxalase 1 (Glo1), the major detoxifying enzyme of MG, in a mechanism involving the redox-sensitive transcription factor Nrf2. Our results suggest that OP holds great promise for the development of preventive strategies for MG-derived AGEs-associated oral diseases and open new paths in research concerning additional studies on the protective potential of this secoiridoid.

scholarly journals The effect of fibroblast growth factor 7 on human dental pulp stem cells for differentiation to AQP5 ‐positive and α SMA ‐positive cells in vitro and in vivo

2021 ◽  
Author(s):  
Yoshihiko Akashi ◽  
Atsushi Nemoto ◽  
Kei Nakajima ◽  
Katsutoshi Kokubun ◽  
Satoshi Murakami ◽  
...  
Keyword(s):  
Stem Cells ◽  
Growth Factor ◽  
Fibroblast Growth Factor ◽  
Dental Pulp ◽  
Dental Pulp Stem Cells ◽  
Fibroblast Growth ◽  
Human Dental Pulp

scholarly journals Cdc42 is essential for the polarized movement and adhesion of human dental pulp stem cells

2018 ◽  
Vol 85 ◽  
pp. 104-112 ◽  
Author(s):  
Mingwei Li ◽  
Liang Ma ◽  
Bing Song ◽  
Dingyi Yu ◽  
Min Xiao ◽  
...  
Keyword(s):  
Stem Cells ◽  
Dental Pulp ◽  
Dental Pulp Stem Cells ◽  
Human Dental Pulp
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