scholarly journals Asymmetric Disulfanylbenzamides as Irreversible and Selective Inhibitors of Staphylococcus aureus Sortase A

ChemMedChem ◽  
2020 ◽  
Vol 15 (10) ◽  
pp. 839-850 ◽  
Author(s):  
Fabian Barthels ◽  
Gabriella Marincola ◽  
Tessa Marciniak ◽  
Matthias Konhäuser ◽  
Stefan Hammerschmidt ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Sortase A ◽  
Selective Inhibitors

scholarly journals In situ regeneration of bioactive coatings enabled by an evolved Staphylococcus aureus sortase A

2016 ◽  
Vol 7 (1) ◽  
Author(s):  
Hyun Ok Ham ◽  
Zheng Qu ◽  
Carolyn A. Haller ◽  
Brent M. Dorr ◽  
Erbin Dai ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Sortase A ◽  
Bioactive Coatings ◽  
In Situ Regeneration

scholarly journals Combined Pharmacophore and 3D-QSAR Study on A Series of Staphylococcus aureus Sortase A inhibitors

2012 ◽  
Vol 80 (2) ◽  
pp. 300-314 ◽  
Author(s):  
Reaz Uddin ◽  
Mazhar U. Lodhi ◽  
Zaheer Ul-Haq
Keyword(s):  
Staphylococcus Aureus ◽  
3D Qsar ◽  
Sortase A ◽  
Qsar Study

Covalent Sortase A Inhibitor ML346 Prevents Staphylococcus aureus Infection of Galleria mellonella

2021 ◽  
Author(s):  
Xiang-Na Guan ◽  
Tao Zhang ◽  
Teng Yang ◽  
Ze Dong ◽  
Song Yang ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Cell Wall ◽  
Galleria Mellonella ◽  
Surface Proteins ◽  
Sortase A ◽  
Gram Positive ◽  
Gram Positive Bacteria ◽  
Aureus Infection ◽  
Cell Wall Peptidoglycan

The housekeeping sortase A (SrtA), a membrane-associated cysteine transpeptidase, is responsible for anchoring surface proteins to the cell wall peptidoglycan in Gram-positive bacteria. This process is essential for the regulation...

Discovery of natural naphthoquinones as sortase A inhibitors and potential anti‐infective solutions against Staphylococcus aureus

2019 ◽  
Vol 80 (8) ◽  
pp. 1136-1145 ◽  
Author(s):  
Georgiana Nitulescu ◽  
Dragos P. Mihai ◽  
Isabela M. Nicorescu ◽  
Octavian T. Olaru ◽  
Anca Ungurianu ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Sortase A

scholarly journals Sortase mutants with improved protein thermostability and enzymatic activity obtained by consensus design

2019 ◽  
Vol 32 (12) ◽  
pp. 555-564
Author(s):  
Magdalena Wójcik ◽  
Susana Vázquez Torres ◽  
Wim J Quax ◽  
Ykelien L Boersma
Keyword(s):  
Staphylococcus Aureus ◽  
Amino Acid ◽  
Enzymatic Activity ◽  
Sortase A ◽  
Wild Type ◽  
Protein Thermostability ◽  
Consensus Analysis ◽  
Gram Positive Bacteria ◽  
Covalently Linked

Abstract Staphylococcus aureus sortase A (SaSrtA) is an enzyme that anchors proteins to the cell surface of Gram-positive bacteria. During the transpeptidation reaction performed by SaSrtA, proteins containing an N-terminal glycine can be covalently linked to another protein with a C-terminal LPXTG motif (X being any amino acid). Since the sortase reaction can be performed in vitro as well, it has found many applications in biotechnology. Although sortase-mediated ligation has many advantages, SaSrtA is limited by its low enzymatic activity and dependence on Ca2+. In our study, we evaluated the thermodynamic stability of the SaSrtA wild type and found the enzyme to be stable. We applied consensus analysis to further improve the enzyme’s stability while at the same time enhancing the enzyme’s activity. As a result, we found thermodynamically improved, more active and Ca2+-independent mutants. We envision that these new variants can be applied in conjugation reactions in low Ca2+ environments.

scholarly journals Discovery and development of substituted thiadiazoles as inhibitors of Staphylococcus aureus Sortase A

2019 ◽  
Vol 27 (19) ◽  
pp. 115043 ◽  
Author(s):  
Patrick M. Wehrli ◽  
Ivana Uzelac ◽  
Thomas Olsson ◽  
Tomas Jacso ◽  
Daniel Tietze ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Sortase A

scholarly journals Inhibitory effect of a natural phenolic compound, 3-p-trans-coumaroyl-2-hydroxyquinic acid against the attachment phase of biofilm formation of Staphylococcus aureus through targeting sortase A

RSC Advances ◽  
2019 ◽  
Vol 9 (56) ◽  
pp. 32453-32461
Author(s):  
Yan-Ping Wu ◽  
Xiao-Yan Liu ◽  
Jin-Rong Bai ◽  
Hong-Chen Xie ◽  
Si-Liang Ye ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Phenolic Compound ◽  
Biofilm Formation ◽  
Inhibitory Effect ◽  
Sortase A ◽  
Initial Attachment ◽  
Biofilm Development

3-p-trans-Coumaroyl-2-hydroxyquinic acid (CHQA), a natural phenolic compound, prevented Staphylococcus aureus biofilm formation due to the inhibition of the initial attachment stage of biofilm development by targeting sortase A.

scholarly journals Design and Synthesis of Small Molecules as Potent Staphylococcus aureus Sortase A Inhibitors

Antibiotics ◽  
2020 ◽  
Vol 9 (10) ◽  
pp. 706
Author(s):  
Min Woo Ha ◽  
Sung Wook Yi ◽  
Seung-Mann Paek
Keyword(s):  
Staphylococcus Aureus ◽  
Bacterial Infections ◽  
Resistant Bacteria ◽  
Sortase A ◽  
Bacterial Survival ◽  
Promising Alternative ◽  
Design And Synthesis ◽  
New Antibiotics ◽  
Pathogenic Action ◽  
New Generation

The widespread and uncontrollable emergence of antibiotic-resistant bacteria, especially methicillin-resistant Staphylococcus aureus, has promoted a wave of efforts to discover a new generation of antibiotics that prevent or treat bacterial infections neither as bactericides nor bacteriostats. Due to its crucial role in virulence and its nonessentiality in bacterial survival, sortase A has been considered as a great target for new antibiotics. Sortase A inhibitors have emerged as promising alternative antivirulence agents against bacteria. Herein, the structural and preparative aspects of some small synthetic organic compounds that block the pathogenic action of sortase A have been described.

scholarly journals Molecular Mechanism of the Flavonoid Natural Product Dryocrassin ABBA against Staphylococcus aureus Sortase A

Molecules ◽  
2016 ◽  
Vol 21 (11) ◽  
pp. 1428 ◽  
Author(s):  
Bing Zhang ◽  
Xiyan Wang ◽  
Lin Wang ◽  
Shuiye Chen ◽  
Dongxue Shi ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Natural Product ◽  
Molecular Mechanism ◽  
Sortase A
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