Combating multidrug‐resistant bacteria by integrating a novel target site penetration and receptor binding assay platform into translational modeling

Background: Multidrug-resistant bacteria can lead to treatment failure, resulting in infectious diseases being transferred through nonpotable water. Aminoglycosides are an important class of antibiotics that are abused in Nigeria. Few studies have investigated aminoglycoside-modifying genes (AMGs) that are likely responsible for resistance in Nigeria bacteria isolates. Therefore, we aimed to characterize AMGs from isolates in drinking water distribution systems (DWDS) in southwestern Nigeria. Methods: Multidrug-resistant bacteria (n = 181) that had been previously characterized by 16S rDNA sequencing and that were positive for resistance to at least 1 aminoglycoside antibiotic were selected from 6 treated and untreated water distribution systems. Strains were PCR genotyped for 3 AMGs: aph(3)c, ant(3)b and aph(6)-1dd. Results: Of 181 MDR bacteria tested, 69 (38.12%) were positive for at least 1 of the AMGs. The most common was ant(3)c (27.6%), followed by aph(3")c (18.23%). Both aph(3)c and ant(3")b were found in 7.73% of tested isolates, ant(3)b was most commonly found in Alcaligenes spp (50%). Furthermore, aph(3")c was most commonly detected in Proteus spp (50%). Other genera positive for AMGs included Acinetobacter, Aeromonas, Bordetella, Brevundimonas, Chromobacterium, Klebsiella, Leucobacter, Morganella, Pantoae, Proteus, Providencia, Psychrobacter, and Serratia. Conclusions: High occurrence of ant(3)c and aph(3)c among these bacteria call for urgent attention among public health workers because these genes can be easily disseminated to consumers if present on mobile genetic elements like plasmids, integrons, and transposons.Funding: NoneDisclosures: None

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Pharmacological Properties of Biogenically Synthesized Silver Nanoparticles using Endophyte Bacillus cereus extract of Berberis lyceum against Oxidative Stress and Pathogenic Multidrug-Resistant Bacteria

Saudi Journal of Biological Sciences ◽

10.1016/j.sjbs.2021.07.009 ◽

2021 ◽

Author(s):

Neelam Mujaddidi ◽

Sobia Nisa ◽

Samha Al Ayoubi ◽

Yamin Bibi ◽

Salman Khan ◽

...

Keyword(s):

Oxidative Stress ◽

Silver Nanoparticles ◽

Bacillus Cereus ◽

Multidrug Resistant ◽

Resistant Bacteria ◽

Pharmacological Properties ◽

Multidrug Resistant Bacteria

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The Antibacterial Activity of Human Amniotic Membrane against Multidrug-Resistant Bacteria Associated with Urinary Tract Infections: New Insights from Normal and Cancerous Urothelial Models

Biomedicines ◽

10.3390/biomedicines9020218 ◽

2021 ◽

Vol 9 (2) ◽

pp. 218

Author(s):

Taja Železnik Ramuta ◽

Larisa Tratnjek ◽

Aleksandar Janev ◽

Katja Seme ◽

Marjanca Starčič Erjavec ◽

...

Keyword(s):

Antibacterial Activity ◽

Urinary Tract ◽

Urinary Tract Infections ◽

Amniotic Membrane ◽

Multidrug Resistant ◽

Basic Knowledge ◽

Resistant Bacteria ◽

Human Amniotic Membrane ◽

Multidrug Resistant Bacteria ◽

Tract Infections

Urinary tract infections (UTIs) represent a serious global health issue, especially due to emerging multidrug-resistant UTI-causing bacteria. Recently, we showed that the human amniotic membrane (hAM) could be a candidate for treatments and prevention of UPEC and Staphylococcus aureus infections. However, its role against multidrug-resistant bacteria, namely methicillin-resistant S. aureus (MRSA), extended-spectrum beta-lactamases (ESBL) producing Escherichia coli and Klebsiella pneumoniae, vancomycin-resistant Enterococci (VRE), carbapenem-resistant Acinetobacter baumannii, and Pseudomonas aeruginosa has not yet been thoroughly explored. Here, we demonstrate for the first time that the hAM homogenate had antibacterial activity against 7 out of 11 tested multidrug-resistant strains, the greatest effect was on MRSA. Using novel approaches, its activity against MRSA was further evaluated in a complex microenvironment of normal and cancerous urinary bladder urothelia. Even short-term incubation in hAM homogenate significantly decreased the number of bacteria in MRSA-infected urothelial models, while it did not affect the viability, number, and ultrastructure of urothelial cells. The hAM patches had no antibacterial activity against any of the tested strains, which further exposes the importance of the hAM preparation. Our study substantially contributes to basic knowledge on the antibacterial activity of hAM and reveals its potential to be used as an antibacterial agent against multidrug-resistant bacteria.

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