scholarly journals Apolipoprotein C‐II induces EMT to promote gastric cancer peritoneal metastasis via PI3K/AKT/mTOR pathway

2021 ◽  
Vol 11 (8) ◽  
Author(s):  
Chao Wang ◽  
Zhi Yang ◽  
En Xu ◽  
Xiaofei Shen ◽  
Xingzhou Wang ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Peritoneal Metastasis ◽  
Mtor Pathway ◽  
Apolipoprotein C

scholarly journals Apolipoprotein C-II induces EMT to Promote Gastric Cancer Peritoneal Metastasis via PI3K/AKT/mTOR Pathway

2021 ◽  
Author(s):  
Chao Wang ◽  
Zhi Yang ◽  
En Xu ◽  
Xingzhou Wang ◽  
Zijian Li ◽  
...  
Keyword(s):  
Gene Expression ◽  
Gastric Cancer ◽  
Lipid Metabolism ◽  
Signaling Pathway ◽  
Peritoneal Metastasis ◽  
Mtor Signaling ◽  
Tandem Mass ◽  
Mtor Signaling Pathway ◽  
Apolipoprotein C

Abstract Background: Peritoneal metastasis (PM) occurs frequently in patients with gastric cancer (GC) and confers poor survival. Lipid metabolism and epithelial-mesenchymal transition (EMT) play an important role in GC metastasis. As Apolipoprotein C-II (APOC2) is a key protein in lipid metabolism, few studies have investigated the role of APOC2 in PM. This study aims to elucidate the potential molecular mechanism of APOC2 in the PM of GC.Methods: The Tandem Mass Tagging (TMT) method followed by liquid chromatography-tandem mass spectrometry-based proteomics analysis was used to compare the levels of differentially expressed proteins between human PM and GC tissues. APOC2 expression was evaluated by immunoblotting, and immunohistochemistry analysis (n = 111). APOC2 over-expression and knock-down expression cell models were developed and tested in vitro. RNA sequencing analysis evaluated the changes in gene expression after APOC2 knockdown in GC cells. The Agilent Seahorse XF platform and lipid staining assay were used to evaluate the role of APOC2 in lipid metabolism of GC cells. Spheroid cell invasion assay, apoptosis assay, colony formation assay, wound-healing assays, and transwell assays were performed and peritoneal implants into nude mice were done to assess the biological effects of APOC2. The underlying mechanisms were investigated using Western blot, inhibitor or activator treatment assays.Results: APOC2 was highly abundant in GC cells and PM tissues. And high APOC2 levels in GC tissues correlated with poor patient prognosis. Knockdown of APOC2 inhibited the malignant phenotype of cancer cells and EMT significantly. Massive gene expression alterations after APOC2 knockdown, which were associated with various signaling pathways, especially the PI3K/AKT signaling pathway and lipid metabolism. Furthermore, the regulatory effects of APOC2 on the EMT were partially attributed to the PI3K/AKT/mTOR signaling pathway. The results in vivo also showed that APOC2 modulated GC PM.Conclusions: We verified that knockdown of APOC2 suppressed GC cell Lipid metabolism, proliferation, migration, invasion, and EMT, accompanied by inactivation of PI3K/AKT/mTOR signaling pathway. APOC2 overexpression had the opposite effects GC cell phenotypes and mechanisms. Collectively, our results identified APOC2 in PM as a potential therapeutic target.

Identification of Significant Genes with Peritoneal Metastasis After Gastric Cancer Surgery via Bioinformatical Analysis

2019 ◽  
Author(s):  
Shengjie Lin ◽  
Tao Jiang ◽  
Guizhen Wen ◽  
Wenyu Gao ◽  
Xuan Gao ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Peritoneal Metastasis ◽  
Cancer Surgery ◽  
Gastric Cancer Surgery ◽  
Bioinformatical Analysis

scholarly journals The Value of Serum Immunoglobulin G Glycome in the Preoperative Discrimination of Peritoneal Metastasis from Advanced Gastric Cancer

2019 ◽  
Vol 10 (12) ◽  
pp. 2811-2821 ◽  
Author(s):  
Ruihuan Qin ◽  
Yupeng Yang ◽  
Wenjun Qin ◽  
Jing Han ◽  
Hao Chen ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Advanced Gastric Cancer ◽  
Immunoglobulin G ◽  
Peritoneal Metastasis ◽  
Serum Immunoglobulin

Identification of novel autoantibodies in ascites of relapsed paclitaxel-resistant gastric cancer with peritoneal metastasis using immunome protein microarrays and proteomics

2021 ◽  
pp. 1-10
Author(s):  
Zhongyin Yang ◽  
Chao Yan ◽  
Wentao Liu ◽  
Wei Xu ◽  
Chen Li ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Effective Treatment ◽  
Peritoneal Metastasis ◽  
Quantitative Proteomics ◽  
Poor Prognosis ◽  
Protein Microarrays ◽  
Tandem Mass ◽  
Tandem Mass Tag ◽  
Resistant Group ◽  
Potential Biomarkers

BACKGROUND: Gastric cancer (GC) patients with peritoneal metastasis usually have extremely poor prognosis. Intraperitoneal infusion of paclitaxel (PTX) provides an effective treatment, but relapse and PTX-resistance are unavoidable disadvantages, and it is difficult to monitor the occurrence of PTX-resistance. OBJECTIVE: The aim of this study was to explore novel autoantibodies in the ascites of individuals with relapsed PTX-resistant GC with peritoneal metastasis. METHODS: Ascites samples were collected before PTX infusion and after the relapse in 3 GC patients. To determine the expression of significantly changed proteins, we performed autoantibody profiling with immunome protein microarrays and tandem mass tag (TMT) quantitative proteomics, and then, the overlapping proteins were selected. RESULTS: Thirty-eight autoantibodies that were differentially expressed between the ascites in the untreated group and relapsed PTX-resistant group were identified. For confirmation of the results, TMT quantitative proteomics was performed, and 842 dysregulated proteins were identified. Four proteins, TPM3, EFHD2, KRT19 and vimentin, overlapped between these two assays. CONCLUSIONS: Our results first revealed that TPM3, EFHD2, KRT19 and vimentin were novel autoantibodies in the ascites of relapsed PTX-resistant GC patients. These autoantibodies may be used as potential biomarkers to monitor the occurrence of PTX-resistance.

scholarly journals Matrix metalloprotease–14 is a Target Enzyme for Detecting Peritoneal Metastasis in Gastric Cancer

2021 ◽  
pp. 102420
Author(s):  
Soichiro Ogawa ◽  
Hidemasa Kubo ◽  
Yasutoshi Murayama ◽  
Takeshi Kubota ◽  
Masayuki Yubakami ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Peritoneal Metastasis ◽  
Matrix Metalloprotease ◽  
Target Enzyme

Genetic variants in PI3K/Akt/mTOR pathway genes contribute to gastric cancer risk

Gene ◽  
2018 ◽  
Vol 670 ◽  
pp. 130-135 ◽  
Author(s):  
Yuqiu Ge ◽  
Hanting Liu ◽  
Xiaonan Qiu ◽  
Gaoxiang Ma ◽  
Haixiao Wang ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Cancer Risk ◽  
Genetic Variants ◽  
Mtor Pathway ◽  
Gastric Cancer Risk

A Bounding Box-Based Radiomic Model for Detecting Occult Peritoneal Metastasis in Advanced Gastric Cancer: A Multicenter Study

2021 ◽  
Author(s):  
Dan Liu ◽  
Weihan Zhang ◽  
Fubi Hu ◽  
Pengxin Yu ◽  
Lanqing Yang ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Advanced Gastric Cancer ◽  
Multicenter Study ◽  
Peritoneal Metastasis ◽  
Bounding Box

Destruction of gastric cancer cells to mesothelial cells by apoptosis in the early peritoneal metastasis

2009 ◽  
Vol 29 (2) ◽  
pp. 163-168 ◽  
Author(s):  
Di Na ◽  
Funan Liu ◽  
Zhifeng Miao ◽  
Zongmin Du ◽  
Huimian Xu
Keyword(s):  
Gastric Cancer ◽  
Cancer Cells ◽  
Peritoneal Metastasis ◽  
Mesothelial Cells ◽  
Gastric Cancer Cells
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