Identification of genital human papillomaviruses in cervical biopsy specimens: Segregation of specific virus types in specific clinicopathologic lesions

1995 ◽  
Vol 61 (1) ◽  
pp. 13-22 ◽  
Author(s):  
Toshihiko Matsukura ◽  
Motoyasu Sugase
Keyword(s):  
Human Papillomaviruses ◽  
Cervical Biopsy ◽  
Biopsy Specimens ◽  
Specific Virus

scholarly journals Variability of Pathologists' Utilization of p16 and Ki-67 Immunostaining in the Diagnosis of Cervical Biopsies in Routine Pathology Practice and Its Impact on the Frequencies of Cervical Intraepithelial Neoplasia Diagnoses and Cytohistologic Correlations

2014 ◽  
Vol 138 (1) ◽  
pp. 76-87 ◽  
Author(s):  
Charanjeet Singh ◽  
J. Carlos Manivel ◽  
Alexander M. Truskinovsky ◽  
Kay Savik ◽  
Samy Amirouche ◽  
...  
Keyword(s):  
Cervical Intraepithelial Neoplasia ◽  
Intraepithelial Neoplasia ◽  
Lower Range ◽  
Ki 67 ◽  
Detection Rates ◽  
Cervical Biopsy ◽  
Biopsy Specimens ◽  
Frequency Of Use ◽  
Lower Variability ◽  
The Impact

Context.—The use of p16 in cervical biopsies improves the accuracy of cervical intraepithelial neoplasia (CIN) diagnosis and grading and decreases its interpathologist variability. Objective.—To determine the impact of the frequency of use of p16 immunostains in cervical biopsies on pathologists' diagnoses of CIN grade 1 and grade 2 or above (CIN1 and CIN2+) and on cytohistologic correlations. Design.—We identified all cervical biopsy specimens with cytologic correlations subjected or not to p16 staining from January 1, 2005, to September 30, 2010; calculated each pathologist's percentage of p16 use; and correlated it with their major cytohistologic discrepancy rates, CIN2+ diagnoses, and CIN1/CIN2+ ratios. Results.—During the study period, each of the 23 pathologists interpreted 59 to 1811 (mean, 518) of 11 850 cervical biopsy specimens, used p16 for 0% to 21.31% (mean, 10.14%) of these, had CIN2+ detection rates of 9.5% to 24.1% (mean, 18.9%), and CIN1/CIN2+ ratios of 0.7 to 4.5 (mean, 1.5). Compared to the 12 “low users” of p16, who used p16 fewer times than the institution's mean for p16 use, the 11 “high users” of p16 diagnosed more biopsies (8391 versus 3459), had a lower rate of major cytohistologic discrepancies (12.62% versus 14.92%, P < .001), a higher rate of CIN2+ diagnoses (19.9% versus 16.4%, P < .001), a lower range of CIN2+ rates (15.0%–23.1% versus 9.5%–24.1%), and lower CIN1/CIN2+ ratios (1.2 versus 2.3). Conclusions.—We found a high intrainstitutional variability of p16 use in cervical biopsies, CIN2+ rates, and CIN1/CIN2+ ratios. Use of p16 for greater than 10% of cervical biopsies was associated with improved cytohistologic correlation rates and with lower variability in the frequencies of histologic diagnoses.

Review of Cervical Carcinoma Screening Program in Tamil Nadu – Current Trend and Recommendations from a Histopathologist’s Viewpoint

2017 ◽  
Vol 5 (1) ◽  
pp. 1-7
Author(s):  
Shifa S Ibrahim ◽  
Kamaleshwari Kesavaraj ◽  
Muthumani Arun ◽  
SA Mohamed Ameen ◽  
Raasi Sankar
Keyword(s):  
Cervical Carcinoma ◽  
Hpv Vaccine ◽  
Tamil Nadu ◽  
Visual Inspection ◽  
Screening Program ◽  
Current Trend ◽  
High Grade Dysplasia ◽  
High Grade ◽  
Cervical Biopsy ◽  
Biopsy Specimens

ABSTRACT Objectives Cervical carcinoma, the commonest carcinoma affecting Indian females, is caused by human papilloma virus (HPV) infection. Primordial prevention and primary prevention with HPV vaccine and cancer screening respectively, can go a long way in preventing this carcinoma. The health system project in Tamil Nadu has done a commendable job in reducing the disease burden by introducing screening programs for cervical carcinoma at the grassroots level, way back in 2005. This study was done to evaluate the cervical biopsy specimens received as a part of this program to compute its incidence, compare the incidence among various districts, and suggest future directions based on our observations. Materials and methods From visual inspection with acetic acid/visual inspection with Lugol’s iodine positive cervical biopsy specimens, 506 were chosen randomly from various districts. Based on histopathological examination, incidence of individual lesions and district-wise incidence were calculated. Predictive factors that determine the progression of these lesions were analyzed based on the literature. Results Out of the 506 cervical biopsy specimens, 34 were unsatisfactory. The incidence of high-grade dysplasia peaked around 31 to 40 years, and squamous cell carcinoma peaked among 51 to 60 years. Madurai ranked high in the incidence of both high-grade dysplasia and carcinoma. Conclusion Incidence of dysplasia and carcinoma in our study was comparable to those seen in the literature. Integration of HPV deoxyribonucleic acid studies into the program can increase the detection rate, detect the progressors, help to identify the HPV species prevalent in an area, and aid in formulating cost-effective HPV vaccine cocktail. How to cite this article Ibrahim SS, Kesavaraj K, Arun M, Ameen SAM, Sankar R. Review of Cervical Carcinoma Screening Program in Tamil Nadu – Current Trend and Recommendations from a Histopathologist’s Viewpoint. J South Asian Feder Menopause Soc 2017;5(1):1-7.

scholarly journals Variance in the Interpretation of Cervical Biopsy Specimens Obtained for Atypical Squamous Cells of Undetermined Significance

2000 ◽  
Vol 114 (5) ◽  
pp. 735-740 ◽  
Author(s):  
Ronald T. Grenko ◽  
Catherine S. Abendroth ◽  
Elizabeth E. Frauenhoffer ◽  
Francesca M. Ruggiero ◽  
Richard J. Zaino
Keyword(s):  
Squamous Cells ◽  
Cervical Biopsy ◽  
Biopsy Specimens ◽  
Undetermined Significance

scholarly journals Observer variability in histopathological reporting of cervical biopsy specimens.

1989 ◽  
Vol 42 (3) ◽  
pp. 231-238 ◽  
Author(s):  
A J Robertson ◽  
J M Anderson ◽  
J S Beck ◽  
R A Burnett ◽  
S R Howatson ◽  
...  
Keyword(s):  
Observer Variability ◽  
Cervical Biopsy ◽  
Biopsy Specimens

Downregulation of IL-2 and IL-23 in Cervical Biopsies of Cervical Intraepithelial Lesions: A Cross-Sectional Study

2020 ◽  
Vol 64 (5) ◽  
pp. 442-451
Author(s):  
Caetano Galvão Petrini ◽  
Larissa Brito Bastos ◽  
Geraldo Duarte ◽  
Patricia Pereira Dos Santos Melli ◽  
José Carlos Alves-Filho ◽  
...  
Keyword(s):  
Immune Response ◽  
Cross Sectional Study ◽  
Low Grade ◽  
Sectional Study ◽  
Cross Sectional ◽  
Cervical Biopsy ◽  
Biopsy Specimens ◽  
Cytokine Levels ◽  
Intraepithelial Lesion ◽  
Intraepithelial Lesions

Introduction: Persistent infection with high-risk human papillomavirus (HPV) types is associated with high-grade intraepithelial lesions (HSILs) and invasive cervical cancer. The host immune response plays a key role in whether HPV clears or persists. Most studies on local immune response to HPV collect cervical mucus in order to quantify secreted cytokines; however, cells located inside the tissue can release different cytokines associated with HPV infection. Objective: This study compared the cytokine levels in cervical biopsy specimens of women with abnormal colposcopic findings according to the histopathological results: low-grade intraepithelial lesion (LSIL), HSIL, and no intraepithelial lesion (NSIL). Methods: A cross-sectional study enrolling 141 cervical biopsy specimens examined the cytokine profile for interleukin (IL-) 2, IL-4, IL-10, IL-12, IL-17, and IL-23 and interferon-γ, using the Luminex assay/ELISA. Differences in cytokine levels among the cervical lesion groups were assessed using the Kruskal-Wallis test. Results: The 141 specimens included 90 HSILs, 22 LSILs, and 29 NSILs. IL-2 levels were significantly higher in NSIL samples than in LSIL or in HSIL samples (p = 0.0001) and IL-23 levels were significantly higher in NSIL than in HSIL samples (p = 0.003). Conclusions: Our study shows that in samples from the lesion site point, 2 important pro-inflammatory cytokines, IL-2 and IL-23, are downregulated in HPV lesions.

Detection of Human Papillomavirus in Cervical Swab Samples by ViraPap and in Cervical Biopsy Specimens byIn SituHybridization

1993 ◽  
Vol 100 (1) ◽  
pp. 12-17 ◽  
Author(s):  
Michael P. Meyer ◽  
Rafael I. Carbonell ◽  
Nancy A. Mauser ◽  
Anisa I. Kanbour ◽  
Antonio J. Amortegui
Keyword(s):  
Human Papillomavirus ◽  
Cervical Biopsy ◽  
Biopsy Specimens ◽  
Cervical Swab

Detection of HPV in Norwegian cervical biopsy specimens with type-specific PCR and reverse line blot assays

2006 ◽  
Vol 36 (4) ◽  
pp. 277-282 ◽  
Author(s):  
Christine C. Roberts ◽  
Amha S. Tadesse ◽  
Jeffrey Sands ◽  
Tore Halvorsen ◽  
Timothy L. Schofield ◽  
...  
Keyword(s):  
Reverse Line Blot ◽  
Specific Pcr ◽  
Cervical Biopsy ◽  
Biopsy Specimens

scholarly journals P16INK4a Expression of Uterine Cervical Biopsy Specimen in SBMCH

2021 ◽  
pp. 1-11
Author(s):  
Tongbram Soni Devi ◽  
Mary Lilly
Keyword(s):  
Biopsy Specimen ◽  
Suppressor Gene ◽  
Tumour Suppressor Gene ◽  
Tumour Suppressor ◽  
Premalignant Lesions ◽  
P16ink4a Expression ◽  
Cervical Biopsy ◽  
Biopsy Specimens ◽  
Neoplastic Lesions ◽  
Endocervical Canal

Introduction: The cervix is the lower portion of the uterus which connects this organ to the vagina through the endocervical canal. It is divided into the vagina (portiovaginalis) and one that lies above the vaginal vault (supravaginal portion). The outer surface of the portiovaginalis is known as the  ectocervix or exocervix and the portion related to the endocervical canal corresponds to the endocervix. Carcinoma cervix accounts for the most common form of cancer by 13% of all the women affected cancers. The P16INK 4a is  a tumour suppressor protein which is a CDKN2A gene product, an  inhibitor  of  cyclin dependent kinase (CDK) 4 and 6 which is encoded  by  tumour suppressor gene INK 4a. Objective: The present study is to evaluate the P16INK 4a expression in cervical biopsy specimens and to correlate it with the histopatholgical findings. Materials and Methods: Cervical biopsy specimens were processed using rotary microtome and tissue blocks cut in sections of 3-5 μm was taken. Results: P16INK4a expression on cervical biopsy specimen showed that those with inflammatory cervical histology, benign lesions of the cervix along with premalignant lesions of cervix are negative for P 16INK4a and all malignant lesions are P 16INK4a positive. Conclusion: Hence P16INK4a immunoreactivity may be used for the diagnosis of neoplastic lesions of the cervix. But in our study, only a small size sample was positive. So, P16INK4a is used as one of the valuable markers for diagnosing neoplastic lesions of the cervix.

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