The cell membrane as the barrier in the defense against nanoxenobiotics: Zinc oxide nanoparticles interactions with native and model membrane of melanoma cells

Author(s):  
Agnieszka Czyżowska ◽  
Anna Barbasz ◽  
Elżbieta Rudolphi‐Szydło ◽  
Barbara Dyba
2020 ◽  
Vol 3 (1) ◽  
pp. 15-27
Author(s):  
Priyankari Bhattacharya ◽  
Kasturi Chatterjee ◽  
Snehasikta Swarnakar ◽  
Sathi Banerjee

Application of metal oxide nanoparticles for treatment of melanoma cells and microbes is being investigated. Zinc oxide nanoparticles (ZnO NPs) deserve special mention where particles cause destruction of melanoma cells with minimal damage to healthy cells. In the present study, pure phase ZnO NPs with particle size of 3.1 nm were synthesized by green route using algal extract. Skin melanoma (B16F10) cells were treated with synthesized ZnO NP and compared with commercial ZnO NPs and analysed for ED50 for cellular viability using 3% (w/v) of the doses. Sensitivity of B16F10 cells towards green synthesized ZnO NP was found to be more than commercial ZnO NPs. Results showed greater reduction in viability of cells exposed to green synthesized ZnO NPs and with increasing dose of the ZnO NPs, percentage viability of cells gradually reduced. 50% decrease in cellular viability (ED50) was obtained for green synthesized ZnO NP at 3% dose while commercial ZnO exhibited ED50 at 6% of doses. The ZnO NP also showed antimicrobial activity against Pseudomonas sp. and Staphylococcus sp. Zone of inhibition (ZOI) exhibited by Pseudomonas aeruginosa and Staphylococcus aureus for disc diffusion and well diffusion assay was around 10-22 mm and 9-12mm respectively.


2014 ◽  
Vol 29 (10) ◽  
pp. 1039
Author(s):  
TAN Man-Lin ◽  
WANG Yan-Tao ◽  
ZHANG Wei-Li ◽  
FU Dong-Ju ◽  
LI Dong-Shuang ◽  
...  

Author(s):  
Hams H. H. Alfattli ◽  
Ghufran Zuhair Jiber ◽  
Ghaidaa Gatea Abbass

This study which designed to evaluate the inhibitory effect of Ethanolic extract of (Quercusrobur) and Zinc oxide nanoparticles on the growth of one genus of enterobacteriacae (Salmonella). In vitro. For this purpose graduate concentrates for plant extract (50, 100, 200, 400 )mg/ml which prepared and compared with Zinc oxide nanoparticles of different concentration (2, 1, 0.5, 0.25) μg/ml,and examined. The result showed that the studied medicinal plant has antibacterial activity against this bacteria which used. The result showed that the plant has good activity in decrease the growth of this bacteria. The results of the study also showed that the nano-ZnO has very effective antibacterial action against the studied bacteria which was Salmonella,nanoparticles concentrations lead to increasing in the inhibition zones of tested bacterial growth. We also study the effect of three antibiotics Lomefloxacin (LOM), Ciprofloxacin (SIP) and Rifampin (RA) and the result showed,in a comparison within the tested bacteria,Salmonella had a significant inhibition increase in Lomefloxacin ; the ciprofloxacin showed effect on tested bacteria. However,Rifampin does not show any effect on tested bacteria.


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