Complex dependence of substrate stiffness and serum concentration on cell-force generation

2006 ◽  
Vol 78A (2) ◽  
pp. 407-415 ◽  
Author(s):  
D. Karamichos ◽  
R.A. Brown ◽  
V. Mudera
Keyword(s):  
Serum Concentration ◽  
Force Generation ◽  
Substrate Stiffness ◽  
Complex Dependence ◽  
Cell Force

scholarly journals Hair cell force generation does not amplify or tune vibrations within the chicken basilar papilla

2016 ◽  
Vol 7 (1) ◽  
Author(s):  
Anping Xia ◽  
Xiaofang Liu ◽  
Patrick D. Raphael ◽  
Brian E. Applegate ◽  
John S. Oghalai
Keyword(s):  
Hair Cell ◽  
Force Generation ◽  
Basilar Papilla ◽  
Cell Force

scholarly journals Altered Smooth Muscle Cell Force Generation as a Driver of Thoracic Aortic Aneurysms and Dissections

2017 ◽  
Vol 37 (1) ◽  
pp. 26-34 ◽  
Author(s):  
Dianna M. Milewicz ◽  
Kathleen M. Trybus ◽  
Dong-chuan Guo ◽  
H. Lee Sweeney ◽  
Ellen Regalado ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Smooth Muscle Cell ◽  
Muscle Cell ◽  
Aortic Aneurysms ◽  
Force Generation ◽  
Thoracic Aortic Aneurysms ◽  
Cell Force

Microposts With Embedded Nanowires to Control Substrate Stiffness

2012 ◽  
Author(s):  
Kevin S. Bielawski ◽  
Nathan J. Sniadecki
Keyword(s):  
Mechanical Response ◽  
Substrate Stiffness ◽  
Promising Trend ◽  
Nickel Nanowires ◽  
A Cell ◽  
Cell Force

Cells can sense the stiffness of their substrate and respond through mechanotransduction pathways that lead to changes in cell contractility1. Studies to measure the mechanical response of cells have been primarily performed on passive substrates that do not change stiffness as a cell matures. This paper presents a novel substrate that has local regions of higher stiffness. In the current setup, polymer microposts are stiffened with nickel nanowires in random locations. An experiment was run with cells seeded onto the posts which resulted in a promising trend of an increase in cell force per post as the percentage of stiff posts seen by the cell increased.

Piperazine Derivatives Enhance Epithelial Cell Monolayer Permeability by Increased Cell Force Generation and Loss of Cadherin Structures

2019 ◽  
Vol 6 (1) ◽  
pp. 367-374
Author(s):  
Shiyuan Zheng ◽  
Kirill Lavrenyuk ◽  
Nicholas G. Lamson ◽  
Katherine C. Fein ◽  
Kathryn A. Whitehead ◽  
...  
Keyword(s):  
Epithelial Cell ◽  
Cell Monolayer ◽  
Force Generation ◽  
Piperazine Derivatives ◽  
Monolayer Permeability ◽  
Cell Force

scholarly journals Micropipette force probe to quantify single-cell force generation: application to T-cell activation

2017 ◽  
Vol 28 (23) ◽  
pp. 3229-3239 ◽  
Author(s):  
Anna Sawicka ◽  
Avin Babataheri ◽  
Stéphanie Dogniaux ◽  
Abdul I. Barakat ◽  
David Gonzalez-Rodriguez ◽  
...  
Keyword(s):  
T Cells ◽  
T Cell ◽  
T Cell Activation ◽  
Cell Activation ◽  
Force Generation ◽  
Activation Process ◽  
Cellular Processes ◽  
Pulling Forces ◽  
A New Technique ◽  
Cell Force

In response to engagement of surface molecules, cells generate active forces that regulate many cellular processes. Developing tools that permit gathering mechanical and morphological information on these forces is of the utmost importance. Here we describe a new technique, the micropipette force probe, that uses a micropipette as a flexible cantilever that can aspirate at its tip a bead that is coated with molecules of interest and is brought in contact with the cell. This technique simultaneously allows tracking the resulting changes in cell morphology and mechanics as well as measuring the forces generated by the cell. To illustrate the power of this technique, we applied it to the study of human primary T lymphocytes (T-cells). It allowed the fine monitoring of pushing and pulling forces generated by T-cells in response to various activating antibodies and bending stiffness of the micropipette. We further dissected the sequence of mechanical and morphological events occurring during T-cell activation to model force generation and to reveal heterogeneity in the cell population studied. We also report the first measurement of the changes in Young’s modulus of T-cells during their activation, showing that T-cells stiffen within the first minutes of the activation process.

scholarly journals Presenilin 1 Affects Focal Adhesion Site Formation and Cell Force Generation via c-Src Transcriptional and Posttranslational Regulation

2009 ◽  
Vol 284 (15) ◽  
pp. 10138-10149 ◽  
Author(s):  
Dieter Waschbüsch ◽  
Simone Born ◽  
Verena Niediek ◽  
Norbert Kirchgessner ◽  
Irfan Y. Tamboli ◽  
...  
Keyword(s):  
Focal Adhesion ◽  
Force Generation ◽  
Presenilin 1 ◽  
Site Formation ◽  
Posttranslational Regulation ◽  
Cell Force ◽  
Focal Adhesion Site ◽  
Adhesion Site

scholarly journals A Novel 3D Fibril Force Assay Implicates Src in Tumor Cell Force Generation in Collagen Networks

PLoS ONE ◽  
2013 ◽  
Vol 8 (3) ◽  
pp. e58138 ◽  
Author(s):  
Robert J. Polackwich ◽  
Daniel Koch ◽  
Richard Arevalo ◽  
Anne M. Miermont ◽  
Kathleen J. Jee ◽  
...  
Keyword(s):  
Tumor Cell ◽  
Force Generation ◽  
Cell Force

Fibronexus-Like Adhesion Sites are Present at the Invasive Zones of Human Epidermoid Carcinomas

1982 ◽  
Vol 40 ◽  
pp. 106-109
Author(s):  
Irwin I. Singer
Keyword(s):  
Cell Cycle ◽  
Serum Concentration ◽  
Substrate Binding ◽  
High Serum ◽  
Adhesion Sites ◽  
Substrate Adhesion ◽  
Focal Contacts ◽  
Adhesion Complex ◽  
Low Serum

Our previous results indicate that two types of fibronectin-cytoskeletal associations may be formed at the fibroblast surface: dorsal matrixbinding fibronexuses generated in high serum (5% FBS) cultures, and ventral substrate-adhering units formed in low serum (0.3% FBS) cultures. The substrate-adhering fibronexus consists of at least vinculin (VN) and actin in its cytoplasmic leg, and fibronectin (FN) as one of its major extracellular components. This substrate-adhesion complex is localized in focal contacts, the sites of closest substratum approach visualized with interference reflection microscopy, which appear to be the major points of cell-tosubstrate adhesion. In fibroblasts, the latter substrate-binding complex is characteristic of cultures that are arrested at the G1 phase of the cell cycle due to the low serum concentration in their medium. These arrested fibroblasts are very well spread, flattened, and immobile.

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