Enhanced schwann cell adhesion and elongation on a topographically and chemically modified poly(L-lactic acid) film surface

Poly(L-lactic acid) (PLLA) has been used as a biodegradable vascular scaffold (BVS) material due to high mechanical property, biodegradability, and biocompatibility. However, acidic byproducts from hydrolysis of PLLA reduce the pH after the surrounding implanted area and cause inflammatory responses. As a result, severe inflammation, thrombosis, and in-stent restenosis can occur after implantation by using BVS. Additionally, polymers such as PLLA could not find on X-ray computed tomography (CT) because of low radiopacity. To this end, here, we fabricated PLLA films as the surface of BVS and divided PLLA films into two coating layers. At the first layer, PLLA film was coated by 2,3,5-triiodobenzoic acid (TIBA) and magnesium hydroxide (MH) with poly(D,L-lactic acid) (PDLLA) for radiopaque and neutralization of acidic environment, respectively. The second layer of coated PLLA films is composed of polydopamine (PDA) and then cystamine (Cys) for the generation of nitric oxide (NO) release, which is needed for suppression of smooth muscle cells (SMCs) and proliferation of endothelial cells (ECs). The characterization of the film surface was conducted via various analyses. Through the surface modification of PLLA films, they have multifunctional abilities to overcome problems of BVS effectively such as X-ray penetrability, inflammation, thrombosis, and neointimal hyperplasia. These results suggest that the modification of biodegradable PLLA using TIBA, MH, PDA, and Cys will have important potential in implant applications.

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Cell adhesion behavior of poly(ε-caprolactone)/poly( L -lactic acid) nanofibers scaffold

Materials Letters ◽

10.1016/j.matlet.2016.02.061 ◽

2016 ◽

Vol 171 ◽

pp. 178-181 ◽

Cited By ~ 12

Author(s):

Zeeshan Khatri ◽

Abdul Wahab Jatoi ◽

Farooq Ahmed ◽

Ick-Soo Kim

Keyword(s):

Lactic Acid ◽

Cell Adhesion ◽

Adhesion Behavior

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Adhesion of Phytophthora palmivora zoospores: electron microscopy of cell attachment and cyst wall fibril formation

Journal of Cell Science ◽

10.1242/jcs.18.1.123 ◽

1975 ◽

Vol 18 (1) ◽

pp. 123-132

Author(s):

V.O. Sing ◽

S. Bartnicki-Garcia

Keyword(s):

Electron Microscopy ◽

Cell Adhesion ◽

Cyst Wall ◽

Cell Attachment ◽

Sodium Dodecyl ◽

Film Surface ◽

Phytophthora Palmivora ◽

Thin Sections ◽

Electron Dense Deposit ◽

Dense Deposit

Zoospores of Phytophthora palmivora adhered to a plastic film surface were examined by electron microscopy. Three stages of adhesion were compared: (1) non-adhesive, unencysted zoospores, (2) adhered incipient cysts, and (3) adhered mature cysts. Thin sections of incipient cysts revealed cells attached to the film surface through the partially discharged contents of the so-called peripheral vesicles; this seems to be the first step in cell adhesion. In mature cysts, the adhesive appeared to have been compacted into an electron-dense deposit binding the cyst wall to the plastic surface. The adhesion zone was also examined in face view after lysing attached incipient cysts with sodium dodecyl sulphate. Cyst wall microfibrils were seen together with an amorphous substance (presumably the adhesive material). The microfibrils were in various stages of formation. Seemingly, adhesion and microfibril formation take place concurrently. The possibility was considered that the material contained in the peripheral vesicles serves in both cell adhesion and microfibril elaboration.

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Lactic Acid Downregulates Viral MicroRNA To Promote Epstein-Barr Virus-Immortalized B Lymphoblastic Cell Adhesion and Growth

Journal of Virology ◽

10.1128/jvi.00033-18 ◽

2018 ◽

Vol 92 (9) ◽

Cited By ~ 6

Author(s):

Xiaohui Mo ◽

Fang Wei ◽

Yin Tong ◽

Ling Ding ◽

Qing Zhu ◽

...

Keyword(s):

Lactic Acid ◽

Cell Adhesion ◽

B Cells ◽

B Cell ◽

Cancer Progression ◽

Epstein Barr Virus ◽

Lactate Production ◽

Barr Virus ◽

Viral Microrna ◽

Epstein Barr

ABSTRACT High plasma lactate is associated with poor prognosis of many malignancies, but its role in virally mediated cancer progression and underlying molecular mechanisms are unclear. Epstein-Barr virus (EBV), the first human oncogenic virus, causes several cancers, including B-cell lymphoma. Here, we report that lactate dehydrogenase A (LDH-A) expression and lactate production are elevated in EBV-immortalized B lymphoblastic cells, and lactic acid (LA; acidic lactate) at low concentration triggers EBV-infected B-cell adhesion, morphological changes, and proliferation in vitro and in vivo . Moreover, LA-induced responses of EBV-infected B cells uniquely occurs in viral latency type III, and it is dramatically associated with the inhibition of global viral microRNAs, particularly the miR-BHRF1 cluster, and the high expression of SMAD3 , JUN , and COL1A genes. The introduction of miR-BHRF1-1 blocks the LA-induced effects of EBV-infected B cells. Thus, this may be a novel mechanism to explain EBV-immortalized B lymphoblastic cell malignancy in an LA microenvironment. IMPORTANCE The tumor microenvironment is complicated, and lactate, which is created by cell metabolism, contributes to an acidic microenvironment that facilitates cancer progression. However, how LA operates in virus-associated cancers is unclear. Thus, we studied how EBV (the first tumor virus identified in humans; it is associated with many cancers) upregulates the expression of LDH-A and lactate production in B lymphoma cells. Elevated LA induces adhesion and the growth of EBV-infected B cells by inhibiting viral microRNA transcription. Thus, we offer a novel understanding of how EBV utilizes an acidic microenvironment to promote cancer development.

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An Amperometric Electrode Based on Carbon Paste, Chemically Modified with D-Lactate Dehydrogenase, NAD+ and Mediator Containing Polymer for D-Lactic Acid Analysis

Biosensors '94 ◽

10.1016/b978-1-85617-242-4.50147-6 ◽

1994 ◽

pp. 182-183

Author(s):

Hun-Chi Shu ◽

Bo Mattiasson ◽

Geza Nagy ◽

Lo Gorton ◽

Björn Persson ◽

...

Keyword(s):

Lactic Acid ◽

Lactate Dehydrogenase ◽

Carbon Paste ◽

Chemically Modified

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Epithelial cell adhesion on films mimicking surface of polymeric scaffolds of artificial urethra compared to molecular modeling of integrin binding

Journal of Bioactive and Compatible Polymers ◽

10.1177/0883911519843309 ◽

2019 ◽

Vol 34 (3) ◽

pp. 280-290 ◽

Cited By ~ 1

Author(s):

Jakub Kollar ◽

Andrea Morelli ◽

Federica Chiellini ◽

Stanislav Miertus ◽

Dusan Bakos ◽

...

Keyword(s):

Hyaluronic Acid ◽

Cell Adhesion ◽

Epithelial Cell ◽

Polymer Films ◽

Rational Design ◽

Computational Study ◽

Transmembrane Protein ◽

Molecular Mechanic ◽

Three Dimensional ◽

Film Surface

In this study, a combined experimental and computational study of long-term human bladder epithelial cell line HBLAK adhesion and proliferation on five different polymeric surfaces, namely hyaluronic acid, amylose, collagen, polyhydroxybutyrate, and polylactic acid, was performed with the goal to understand the nature of the attraction between various surface materials and a simplified model of the cell surface (transmembrane protein integrin). These biodegradable polymers are frequently used as scaffolds for tissue engineering. During formation of the new tissue, the scaffold polymers are gradually replaced by the natural extracellular matrix of the proliferating cells. Cell adhesion and proliferation experiments were carried out employing thin polymer films prepared by solvent casting while for the computational approach three-dimensional molecular models of layers of ordered polymeric fibers were used as quasi-planar nano-sized models of polymeric surface patches. Experimental results indicated a good capability of amylose, polyhydroxybutyrate, and hyaluronic acid polymer films to foster cell adhesion. Proliferation experiment, carried out by incubating cells with the investigated polymer films for 72 h, showed that all the investigated polymers are able to sustain a good proliferation of HBLAK cells almost comparable to plain glass. Computational estimate of molecular mechanic interaction energies between three-dimensional models of polymeric films and the collagen-binding α2 I domain of the cell adhesion receptor integrin α2β1 confirmed elevated affinity to amylose and polyhydroxybutyrate that is related to higher polarity of function groups on the film surface as documented by the maps of molecular electrostatic potential. This combined experimental and modeling approach can contribute to rational design and surface modifications of polymeric material suitable for forming the scaffolds of human urethra that can be effectively colonized by stem cells.

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