Contrasting effects of midazolam on induction of heat shock protein 27 by vasopressin and heat in aortic smooth muscle cells

2001 ◽  
Vol 84 (1) ◽  
pp. 39-46 ◽  
Author(s):  
Kumiko Tanabe ◽  
Osamu Kozawa ◽  
Masayuki Niwa ◽  
Takuji Yamomoto ◽  
Hiroyuki Matsuno ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Heat Shock ◽  
Heat Shock Protein ◽  
Smooth Muscle Cells ◽  
Muscle Cells ◽  
Heat Shock Protein 27 ◽  
Aortic Smooth Muscle Cells ◽  
Aortic Smooth Muscle

scholarly journals Aspirin relieves the calcification of aortic smooth muscle cells by enhancing the heat shock response

2021 ◽  
Vol 60 (1) ◽  
pp. 17-24
Author(s):  
Quanquan Shen ◽  
Qian Chen ◽  
Yang Liu ◽  
Xiang Xue ◽  
Xiaogang Shen ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Heat Shock ◽  
Smooth Muscle Cells ◽  
Heat Shock Response ◽  
Muscle Cells ◽  
Aortic Smooth Muscle Cells ◽  
Aortic Smooth Muscle ◽  
Shock Response

Differential effect of simvastatin on activation of Rac1 vs. activation of the heat shock protein 27-mediated pathway upon oxidative stress, in human smooth muscle cells

2002 ◽  
Vol 64 (10) ◽  
pp. 1483-1491 ◽  
Author(s):  
Pascale Nègre-Aminou ◽  
Rick E.W van Leeuwen ◽  
G.Christa F van Thiel ◽  
Paul van den IJssel ◽  
Wilfried W de Jong ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Smooth Muscle ◽  
Heat Shock ◽  
Heat Shock Protein ◽  
Smooth Muscle Cells ◽  
Differential Effect ◽  
Muscle Cells ◽  
Heat Shock Protein 27

Pentobarbital, but not Propofol, Suppresses Vasopressin-stimulated Heat Shock Protein 27 Induction in Aortic Smooth Muscle Cells

2000 ◽  
Vol 92 (6) ◽  
pp. 1807-1813 ◽  
Author(s):  
Osamu Kozawa ◽  
Kumiko Tanabe ◽  
Hiroyuki Matsuno ◽  
Masayuki Niwa ◽  
Takuji Yamamoto ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Smooth Muscle ◽  
Protein Kinase C ◽  
Heat Shock ◽  
Protein Kinase ◽  
Heat Shock Protein ◽  
Smooth Muscle Cells ◽  
Muscle Cells ◽  
Aortic Smooth Muscle ◽  
Kinase C

Background Although it is known that systemic blood pressure decreases after the administration of pentobarbital or propofol, the mechanisms underlying the cardiovascular effects of these anesthetics are still poorly understood. The authors previously showed that vasopressin stimulates the induction of heat shock protein (HSP) 27, a low-molecular-weight HSP, by a protein kinase C-dependent manner in aortic smooth muscle A10 cells. It is recognized that HSP27 may act as a chaperone like high-molecular-weight HSPs such as HSP70. HSP27 is reportedly associated with agonist-induced contraction of vascular smooth muscle cells. The authors examined the effects of pentobarbital and propofol on the vasopressin-stimulated HSP27 induction in A10 cells. Methods Cultured A10 cells were pretreated with pentobarbital or propofol and then stimulated by vasopressin or 12-o-tetradecanoylphorbol 13-acetate (TPA). The effect of vasopressin on HSP70 was evaluated by Western blot analysis and compared with its effect on HSP27. The concentrations of HSP27 were determined by a specific immunoassay. The effect of pentobarbital on the expression levels of mRNA for HSP27 by vasopressin was evaluated by Northern blot analysis. Results Vasopressin induced HSP27 but had little effect on HSP70. At concentrations used clinically, pentobarbital inhibited the accumulation of HSP27 by vasopressin or TPA. Pentobarbital reduced the levels of mRNA for HSP27 induced by vasopressin. In contrast, propofol affected neither the vasopressin- nor TPA-induced HSP27 accumulation. Conclusions These results suggest that pentobarbital suppresses the vasopressin-stimulated HSP27 induction in vascular smooth muscle cells. This inhibitory effect is probably exerted at a point downstream from protein kinase C.

Sorghum Fermented by Aspergillus oryzae NK Enhances Inhibition of Vascular Inflammation in TNF-α-stimulated Human Aortic Smooth Muscle Cells

2018 ◽  
Vol 88 (5-6) ◽  
pp. 309-318
Author(s):  
Hae Seong Song ◽  
Jung-Eun Kwon ◽  
Hyun Jin Baek ◽  
Chang Won Kim ◽  
Hyelin Jeon ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Inflammatory Response ◽  
Smooth Muscle Cells ◽  
Adhesion Molecule ◽  
Vascular Inflammation ◽  
Muscle Cells ◽  
Aortic Smooth Muscle Cells ◽  
Aortic Smooth Muscle ◽  
Tnf Α ◽  
Cox 2

Abstract. Sorghum bicolor L. Moench is widely grown all over the world for food and feed. The effects of sorghum extracts on general inflammation have been previously studied, but its anti-vascular inflammatory effects are unknown. Therefore, this study investigated the anti-vascular inflammation effects of sorghum extract (SBE) and fermented extract of sorghum (fSBE) on human aortic smooth muscle cells (HASMCs). After the cytotoxicity test of the sorghum extract, a series of experiments were conducted. The inhibition effects of SBE and fSBE on the inflammatory response and adhesion molecule expression were measured using treatment with tumor necrosis factor-α (TNF-α), a crucial promoter for the development of atherosclerotic lesions, on HASMCs. After TNF-α (10 ng/mL) treatment for 2 h, then SBE and fSBE (100 and 200 μg/mL) were applied for 12h. Western blotting analysis showed that the expression of vascular cell adhesion molecule-1 (VCAM-1) (2.4-fold) and cyclooxygenase-2 (COX-2) (6.7-fold) decreased, and heme oxygenase-1 (HO-1) (3.5-fold) increased compared to the TNF-α control when treated with 200 μg/mL fSBE (P<0.05). In addition, the fSBE significantly increased the expression of HO-1 and significantly decreased the expression of VCAM-1 and COX-2 compared to the TNF-α control in mRNA level (P<0.05). These reasons of results might be due to the increased concentrations of procyanidin B1 (about 6-fold) and C1 (about 30-fold) produced through fermentation with Aspergillus oryzae NK for 48 h, at 37 °C. Overall, the results demonstrated that fSBE enhanced the inhibition of the inflammatory response and adherent molecule expression in HASMCs.

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