Detection ofCFTRtransgene mRNA expression in respiratory epithelium isolated from the murine nasal cavity

Aim: to study the morphological patterns of polypous tissue according to cytology and histology. Pateints and methods: 78 patients with a confirmed diagnosis of polypoid rhinosinusitis (PRS) were selected for the study, including 50 men and 28 women aged 25 to 74 years. The material for cytology was scraped from the surface of nasal cavity and paranasal sinus (PNS) polyps, as well as touch smears from the cut surface of removed polyps, and the material for histology was pieces of removed polypous tissue. Results: in the course of the study, the following comparative analysis of the obtained results was made: a) with polyps with a fibrous component in touch smears, there are expressed signs of chronic inflammation along with signs of respiratory epithelial cells proliferation; b) edematous and myxoid polyps are commonly combined with an allergic condition; c) analysis of cytomorphological signs of the respiratory epithelium of the nasal cavity and PNS in PRS indicates a structural alteration of the mucous membrane in this disease. The epithelial proliferation is associated with the activation of stroma components — fibroblasts and leukocytes. At the light-optical level, the characteristics of compensatory and adaptive changes that contribute to the morphofunctional cell unity preservation of the mucous membrane that counteract the pathological process are revealed. Conclusions: according to the method of sampling material for cytology, the most informative are touch smears from the cut surface of the removed polyp, which help to identify more morphological patterns of the polypous tissue. The analysis of cytomorphological features of the respiratory epithelium of the nasal cavity and PNS in PRS indicates the remodeling of the mucous membrane in this disease. The epithelial proliferation is associated with the activation of stroma components — fibroblasts and leukocytes. At the light-optical level, the characteristics of compensatory and adaptive changes that contribute to the morphofunctional cell unity preservation of the mucous membrane that counteract the pathological process are revealed. The obtained results will help to understand the mechanisms of structural and functional alteration in the nasal mucosa and PNS in PRS. KEYWORDS: polypoid rhinosinusitis, cytology, histology, paranasal sinuses, smears, fibroblasts, eosinophils, leukocytes, respiratory epithelium. FOR CITATION: Zakharova N.M., Shabalova I.P., Egorov V.I. et al. Structural reconstruction of the nasal mucosa and paranasal sinuses in patients with polypous rhinosinusitis according to cytological and histological studies. Russian Medical Inquiry. 2021;5(7): 479–485 (in Russ.). DOI: 10.32364/2587-6821-2021-5-7-479-485.

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Structure of Masera's Septal Olfactory Organ in Cat (Felis silvestris f. catus) - Light Microscopy in Selected Stages of Ontogeny

Acta Veterinaria Brno ◽

10.2754/avb200675040471 ◽

2006 ◽

Vol 75 (4) ◽

pp. 471-475

Author(s):

I. Kociánová ◽

A. Gorošová ◽

F. Tichý ◽

P. Čížek ◽

M. Machálka

Keyword(s):

Nasal Cavity ◽

Developmental Stage ◽

Vomeronasal Organ ◽

Sensory Epithelium ◽

Respiratory Epithelium ◽

Domestic Cat ◽

Olfactory Organ ◽

Felis Silvestris ◽

Typical Structure ◽

Surface Appearance

The septal organ /SO/ (Masera's organ /MO/) is a chemoreceptor presently considered one of three types of olfactory organs (along with the principal olfactory region and vomeronasal organ). Notwithstanding the septal organ having been first described by Rodolfo Masera in 1943, little is known of the properties of sensory neurons or of its functional significance in chemoreception. Until now the septal organ has been described only in laboratory rodents and some marsupials. This work refers to its existence in the domestic cat (Felis silvestris f. catus). The septal organ can be identified at the end of embryonic period - 27 or 28 days of ontogenesis in cats (the 6th developmental stage of Štěrba) - coincident with formation of the principal olfactory region in nasal cavity. At 45 days of ontogenesis (the 9th developmental stage of Štěrba), this septal olfactory organ is of circular or oval shape, 120 μm in diameter, in ventral part of septum nasi, lying caudally to the opening of ductus incisivus. The structure of the epithelium of septal olfactory organ is clearly distinct from the respiratory epithelium of the nasal cavity. It varies in thickness, cellular composition, as well as free surface appearance, and even lack the typical structure of sensory epithelium, in this developmental period. Nerve bundles and glandular acini are lacking in the lamina propria mucosae of the septal organ and in the adjacent tissues. Glands appear as the single non-luminized cords of epithelia extending from the surface. The adjacent respiratory epithelium contains numerous goblet cells.

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Early stages of ciliogenesis in the respiratory epithelium of the nasal cavity of rabbit embryos

Cell and Tissue Research ◽

10.1007/bf00218795 ◽

1989 ◽

Vol 255 (3) ◽

Cited By ~ 6

Author(s):

G.P. Loots ◽

P.P.C. Nel

Keyword(s):

Nasal Cavity ◽

Respiratory Epithelium ◽

Early Stages ◽

Rabbit Embryos

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Nonolfactory surface epithelium of the nasal cavity of the bonnet monkey: A morphologic and morphometric study of the transitional and respiratory epithelium

American Journal of Anatomy ◽

10.1002/aja.1001800308 ◽

1987 ◽

Vol 180 (3) ◽

pp. 266-279 ◽

Cited By ~ 46

Author(s):

Jack R. Harkema ◽

Charles G. Plopper ◽

Dallas M. Hyde ◽

Dennis W. Wilson ◽

Judith A. St. George ◽

...

Keyword(s):

Nasal Cavity ◽

Respiratory Epithelium ◽

Morphometric Study ◽

Surface Epithelium ◽

Bonnet Monkey

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Morphogenesis of the rhea (Rhea americana) respiratory system in different embryonic and foetal stages

Pesquisa Veterinária Brasileira ◽

10.1590/1678-5150-pvb-5310 ◽

2018 ◽

Vol 38 (1) ◽

pp. 154-166

Author(s):

Renata P. Sousa ◽

Hatawa M. de A. Monteiro ◽

Dayseanny de O. Bezerra ◽

Leticya L. da S. Soares ◽

Antônio C. Assis Neto ◽

...

Keyword(s):

Nasal Cavity ◽

Respiratory System ◽

Production Systems ◽

Hyaline Cartilage ◽

Goblet Cells ◽

Respiratory Epithelium ◽

Ciliated Epithelium ◽

Foetal Development ◽

Organic System ◽

Rhea Americana

ABSTRACT: The rhea (Rhea americana) is an important wild species that has been highlighted in national and international livestock. This research aims to analyse embryo-foetal development in different phases of the respiratory system of rheas. Twenty-three embryos and foetuses were euthanized, fixed and dissected. Fragments of the respiratory system, including the nasal cavity, larynx, trachea, syrinx, bronchi and lungs, were collected and processed for studies using light and scanning electron microscopy. The nasal cavity presented cubic epithelium in the early stages of development. The larynx exhibited typical respiratory epithelium between 27 and 31 days. The trachea showed early formation of hyaline cartilage after 15 days. Syrinx in the mucous membrane of 18-day foetuses consisted of ciliated epithelium in the bronchial region. The main bronchi had ciliated epithelium with goblet cells in the syringeal region. In the lung, the parabronchial stage presented numerous parabronchi between 15 and 21 days. This study allowed the identification of normal events that occur during the development of the rhea respiratory system, an important model that has not previously been described. The information generated here will be useful for the diagnosis of pathologies that affect this organic system, aimed at improving captive production systems.

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The Presence of Melanocytes in the Nasal Cavity

Annals of Otology Rhinology & Laryngology ◽

10.1177/000348947408300414 ◽

1974 ◽

Vol 83 (4) ◽

pp. 515-519 ◽

Cited By ~ 72

Author(s):

Frederick G. Zak ◽

William Lawson

Keyword(s):

Dendritic Cells ◽

Nasal Cavity ◽

Respiratory Epithelium ◽

Silver Impregnation ◽

Melanin Pigment ◽

Nasal Cavities ◽

Primary Neoplasms ◽

Nasal Glands

Dendritic cells containing melanin pigment have been demonstrated with silver impregnation and bleaching techniques in the nasal cavities of both Caucasians and Negroes. They were found in the respiratory epithelium, nasal glands and commonly in the superficial and deep stroma of the septum and middle and inferior turbinates, particularly in adult Negroes. The presence of melanocytes explains the histogenesis of melanomas of the nasal cavity as primary neoplasms at this site.

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The experience of using Frinosole in the early postoperative period in patients after endonasal endoscopic operations

Russian otorhinolaryngology ◽

10.18692/1810-4800-2020-3-100-104 ◽

2020 ◽

Vol 19 (3) ◽

pp. 100-104

Author(s):

T. A. Mashkova ◽

◽

A. B. Maltsev ◽

Keyword(s):

Nasal Cavity ◽

Mucous Membrane ◽

Nasal Mucosa ◽

Postoperative Period ◽

Respiratory Epithelium ◽

Early Postoperative Period ◽

Absorption Capacity ◽

Postoperative Treatment ◽

Surgical Trauma ◽

Postoperative Changes

The effectiveness of the drug Frinosole in the complex treatment of early postoperative changes in the nasal mucosa after endonasal endoscopic surgery was studied. It has been confirmed that during surgical trauma of the mucous membrane of the nasal cavity, inhibition of the main functions of the respiratory epithelium occurs with a sharp increase in the absorption capacity of the mucous membrane of the nasal cavity. These changes correlate with the clinical picture of the early postoperative period. The data presented indicate a high efficacy of the drug in complex postoperative treatment and make it possible to recommend it for wider use in order to minimize the duration of the postoperative healing stages and prevent possible complications associated with excessive granulation and scarring of the nasal mucosa in the surgical areas.

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Dexamethasone Effect on Embryonic Chick Respiratory Epithelium

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100053905 ◽

1982 ◽

Vol 40 ◽

pp. 248-249

Author(s):

M.R. Richter ◽

R.V. Blystone

Keyword(s):

Lung Development ◽

Critical Role ◽

Respiratory Epithelium ◽

Chick Embryos ◽

Embryonic Chick ◽

White Leghorn ◽

Lung Maturation ◽

Synthetic Analogs ◽

Lung Physiology ◽

Avian Lung

Dexamethasone and other synthetic analogs of corticosteroids have been employed clinically as enhancers of lung development. The mechanism(s) by which this steroid induction of later lung maturation operates is not clear. This study reports the effect on lung epithelia of dexamethasone administered at different intervals during development. White Leghorn chick embryos were used so as to remove possible maternal and placental influences on the exogenously applied steroid. Avian lung architecture does vary from mammals; however, respiratory surfactant produced by the lung epithelia serves an equally critical role in avian lung physiology.

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Bronchial stereocilia in the immotile cilia syndrome: A case report

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100110635 ◽

1984 ◽

Vol 42 ◽

pp. 52-53

Author(s):

George Price ◽

Lizardo Cerezo

Keyword(s):

Surface Modifications ◽

Respiratory Epithelium ◽

Immotile Cilia ◽

Kartagener's Syndrome ◽

Membrane Blebs ◽

Radial Spokes ◽

Males And Females ◽

Dynein Arms ◽

Ultrastructural Finding ◽

Ultrastructural Findings

Ultrastructural defects of ciliary structure have been known to cause recurrent sino-respiratory infection concurrent with Kartagener's syndrome. (1,2,3) These defects are also known to cause infertility in both males and females. (4) Overall, the defects are defined as the Immotile, or Dyskinetic Cilia Syndrome (DCS). Several ultrastructural findings have been described, including decreased number of cilia, multidirection orientation, fused and compound cilia, membrane blebs, excess matrix in the axoneme, missing outer tubular doublets, translocated doublets, defective radial spokes and dynein arms. A rare but noteworthy ultrastructural finding in DCS is the predominance of microvilli-like structures on the luminal surface of the respiratory epithelium. (5,6) These permanent surface modifications of the apical respiratory epithelium no longer resemble cilia but reflect the ultrastructure of stereocilia, similar to that found in the epidydimal epithelium. Like microvilli, stereocilia are devoid of microtubular ultrastructure in comparison with true cilia.

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