A strategy for liquid chromatography/tandem mass spectrometric assays of intracellular drugs: application to the validation of the triphosphorylated anabolite of antiretrovirals in peripheral blood mononuclear cells

2003 ◽  
Vol 38 (8) ◽  
pp. 879-890 ◽  
Author(s):  
Fran�ois Becher ◽  
Alain Pruvost ◽  
Jane Gale ◽  
Philippe Couerbe ◽  
C�cile Goujard ◽  
...  
Keyword(s):  
Liquid Chromatography ◽  
Peripheral Blood Mononuclear Cells ◽  
Peripheral Blood ◽  
Mononuclear Cells ◽  
Mass Spectrometric ◽  
Tandem Mass ◽  
Peripheral Blood Mononuclear ◽  
Blood Mononuclear Cells ◽  
Liquid Chromatography Tandem Mass ◽  
Tandem Mass Spectrometric

scholarly journals Intracellular Efavirenz Levels in Peripheral Blood Mononuclear Cells from Human Immunodeficiency Virus-Infected Individuals

2007 ◽  
Vol 52 (2) ◽  
pp. 782-785 ◽  
Author(s):  
Rie Tanaka ◽  
Hideji Hanabusa ◽  
Ei Kinai ◽  
Naoki Hasegawa ◽  
Masayoshi Negishi ◽  
...  
Keyword(s):  
Human Immunodeficiency Virus ◽  
Peripheral Blood Mononuclear Cells ◽  
Peripheral Blood ◽  
Plasma Levels ◽  
Mononuclear Cells ◽  
Peripheral Blood Mononuclear ◽  
Immunodeficiency Virus ◽  
Blood Mononuclear Cells ◽  
Liquid Chromatography Tandem Mass ◽  
Novel Method

ABSTRACT We describe a novel method for isolating plasma-free peripheral blood mononuclear cells retaining intracellular efavirenz. Quantification of efavirenz in 13 human immunodeficiency virus-infected patients by liquid chromatography-tandem mass spectrometry showed a higher correlation of intracellular levels with unbound plasma levels (accumulation ratio, 1,190) than with total plasma levels.

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