Overexpression of PD‐L1 in gingival basal keratinocytes reduces periodontal inflammation in a ligature‐induced periodontitis model

The effectiveness of the immunomodulator Polymuramil (PM) was determined in the treatment of 40 patients with severe chronic generalized periodontitis based on the dynamics of the values of periodontal indices and the content of periodontal pathogens in the periodontal pockets. Patients of the main group (20 people) were treated with professional hygiene (PGO) in combination with daily intramuscular PM for 5 days at a dose of 200 mcg. Patients of the control group (20 people) were performed only PGO. The effectiveness of treatment was evaluated based on the dynamics of the clinical indices of Muehlemann and PMA and the presence or absence of major periodontal pathogens in the periodontal pockets. Results: in patients of the main group, the phenomena of periodontal inflammation and the main periodontal pathogens were eliminated in a shorter time and to a greater extent in comparison with patients of the control group

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Association between periodontal inflammation and serum lipid profile in a healthy population: A cross‐sectional study

Journal of Periodontal Research ◽

10.1111/jre.12917 ◽

2021 ◽

Author(s):

Risako Mikami ◽

Koji Mizutani ◽

Yusuke Matsuyama ◽

Takanori Matsuura ◽

Daisuke Kido ◽

...

Keyword(s):

Lipid Profile ◽

Serum Lipid ◽

Cross Sectional Study ◽

Serum Lipid Profile ◽

Healthy Population ◽

Sectional Study ◽

Cross Sectional ◽

Periodontal Inflammation

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A deficiency of Dec2 triggers periodontal inflammation and pyroptosis

Journal of Periodontal Research ◽

10.1111/jre.12849 ◽

2021 ◽

Author(s):

Shunichi Oka ◽

Xiaoyan Li ◽

Fuyuki Sato ◽

Fengzhu Zhang ◽

Nitesh Tewari ◽

...

Keyword(s):

Periodontal Inflammation

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AT1 and AT2 Receptor Knockout Changed Osteonectin and Bone Density in Mice in Periodontal Inflammation Experimental Model

International Journal of Molecular Sciences ◽

10.3390/ijms22105217 ◽

2021 ◽

Vol 22 (10) ◽

pp. 5217

Author(s):

Maria Laura de Souza Lima ◽

Caroline Addison Carvalho Xavier de Medeiros ◽

Gerlane Coelho Bernardo Guerra ◽

Robson Santos ◽

Michael Bader ◽

...

Keyword(s):

Bone Density ◽

Bone Loss ◽

Experimental Model ◽

At2 Receptor ◽

Micro Ct ◽

Rt Pcr ◽

Osteoblast Cells ◽

Periodontal Inflammation ◽

Bone Trabeculae

Background: The aim of this study was to evaluate the role of AT1 and AT2 receptors in a periodontal inflammation experimental model. Methods: Periodontal inflammation was induced by LPS/Porphyromonas gingivalis. Maxillae, femur, and vertebra were scanned using Micro-CT. Maxillae were analyzed histopathologically, immunohistochemically, and by RT-PCR. Results: The vertebra showed decreased BMD in AT1 H compared with WT H (p < 0.05). The femur showed increased Tb.Sp for AT1 H and AT2 H, p < 0.01 and p < 0.05, respectively. The Tb.N was decreased in the vertebra (WT H-AT1 H: p < 0.05; WT H-AT2 H: p < 0.05) and in the femur (WT H-AT1 H: p < 0.01; WT H-AT2 H: p < 0.05). AT1 PD increased linear bone loss (p < 0.05) and decreased osteoblast cells (p < 0.05). RANKL immunostaining was intense for AT1 PD and WT PD (p < 0.001). OPG was intense in the WT H, WT PD, and AT2 PD when compared to AT1 PD (p < 0.001). AT1 PD showed weak immunostaining for osteocalcin compared with WT H, WT PD, and AT2 PD (p < 0.001). AT1 H showed significantly stronger immunostaining for osteonectin in fibroblasts compared to AT2 H (p < 0.01). Conclusion: AT1 receptor knockout changed bone density, the quality and number of bone trabeculae, decreased the number of osteoblast cells, and increased osteonectin in fibroblasts.

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Lipid, fatty acid, carnitine- and choline derivative profiles in rheumatoid arthritis outpatients with different degrees of periodontal inflammation

Scientific Reports ◽

10.1038/s41598-021-84122-y ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Kathrin Beyer ◽

Stein Atle Lie ◽

Bodil Bjørndal ◽

Rolf K. Berge ◽

Asbjørn Svardal ◽

...

Keyword(s):

Rheumatoid Arthritis ◽

Fatty Acid ◽

Mitochondrial Dysfunction ◽

Inflammatory Diseases ◽

Whole Body ◽

Cross Sectional ◽

Chronic Inflammatory Diseases ◽

Periodontal Inflammation ◽

Inflammatory Status ◽

Lipid Fatty Acid

AbstractRheumatoid arthritis (RA) and periodontitis are chronic inflammatory diseases with several pathogenic pathways in common. Evidence supports an association between the diseases, but the exact underlying mechanisms behind the connection are still under investigation. Lipid, fatty acid (FA) and metabolic profile alterations have been associated with several chronic inflammatory diseases, including RA and periodontitis. Mitochondria have a central role in regulating cellular bioenergetic and whole-body metabolic homeostasis, and mitochondrial dysfunction has been proposed as a possible link between the two disorders. The aim of this cross-sectional study was to explore whole-blood FA, serum lipid composition, and carnitine- and choline derivatives in 78 RA outpatients with different degrees of periodontal inflammation. The main findings were alterations in lipid, FA, and carnitine- and choline derivative profiles. More specifically, higher total FA and total cholesterol concentrations were found in active RA. Elevated phospholipid concentrations with concomitant lower choline, elevated medium-chain acylcarnitines (MC-AC), and decreased ratios of MC-AC and long-chain (LC)-AC were associated with prednisolone medication. This may indicate an altered mitochondrial function in relation to the increased inflammatory status in RA disease. Our findings may support the need for interdisciplinary collaboration within the field of medicine and dentistry in patient stratification to improve personalized treatment. Longitudinal studies should be conducted to further assess the potential impact of mitochondrial dysfunction on RA and periodontitis.

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MicroRNAs: Harbingers and shapers of periodontal inflammation

Seminars in Cell and Developmental Biology ◽

10.1016/j.semcdb.2021.05.030 ◽

2021 ◽

Author(s):

Xianghong Luan ◽

Xiaofeng Zhou ◽

Pooria Fallah ◽

Mirali Pandya ◽

Huling Lyu ◽

...

Keyword(s):

Periodontal Inflammation

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Interaction of periodontitis and orthodontic tooth movement—an in vitro and in vivo study

Clinical Oral Investigations ◽

10.1007/s00784-021-03988-4 ◽

2021 ◽

Author(s):

Birgit Rath-Deschner ◽

Andressa V. B. Nogueira ◽

Svenja Beisel-Memmert ◽

Marjan Nokhbehsaim ◽

Sigrun Eick ◽

...

Keyword(s):

Alveolar Bone ◽

Tooth Movement ◽

Mechanical Strain ◽

Orthodontic Tooth Movement ◽

Fusobacterium Nucleatum ◽

In Vivo Study ◽

Rt Pcr ◽

Periodontal Inflammation

Abstract Objectives The aim of this in vitro and in vivo study was to investigate the interaction of periodontitis and orthodontic tooth movement on interleukin (IL)-6 and C-X-C motif chemokine 2 (CXCL2). Materials and methods The effect of periodontitis and/or orthodontic tooth movement (OTM) on alveolar bone and gingival IL-6 and CXCL2 expressions was studied in rats by histology and RT-PCR, respectively. The animals were assigned to four groups (control, periodontitis, OTM, and combination of periodontitis and OTM). The IL-6 and CXCL2 levels were also studied in human gingival biopsies from periodontally healthy and periodontitis subjects by RT-PCR and immunohistochemistry. Additionally, the synthesis of IL-6 and CXCL2 in response to the periodontopathogen Fusobacterium nucleatum and/or mechanical strain was studied in periodontal fibroblasts by RT-PCR and ELISA. Results Periodontitis caused an increase in gingival levels of IL-6 and CXCL2 in the animal model. Moreover, orthodontic tooth movement further enhanced the bacteria-induced periodontal destruction and gingival IL-6 gene expression. Elevated IL-6 and CXCL2 gingival levels were also found in human periodontitis. Furthermore, mechanical strain increased the stimulatory effect of F. nucleatum on IL-6 protein in vitro. Conclusions Our study suggests that orthodontic tooth movement can enhance bacteria-induced periodontal inflammation and thus destruction and that IL-6 may play a pivotal role in this process. Clinical relevance Orthodontic tooth movement should only be performed after periodontal therapy. In case of periodontitis relapse, orthodontic therapy should be suspended until the periodontal inflammation has been successfully treated and thus the periodontal disease is controlled again.

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