Response of laying Japanese quails to graded levels of essential amino acids profile with reduced dietary protein

2007 ◽  
Vol 87 (5) ◽  
pp. 751-759 ◽  
Author(s):  
Sarabmeet Kaur ◽  
Asit B Mandal ◽  
Kunwer B Singh ◽  
Mukund M Kadam ◽  
Arumbackam V Elangovan
Keyword(s):  
Amino Acids ◽  
Dietary Protein ◽  
Essential Amino Acids ◽  
Japanese Quails ◽  
Amino Acids Profile

P–283 Combination between amino acids profile of the spent culture media and morphokinetics parameters of human embryos to determine its viability

2021 ◽  
Vol 36 (Supplement_1) ◽  
Author(s):  
N Adel ◽  
M Kadah ◽  
S Abdulghafar ◽  
M Elmahdy ◽  
D Ghareeb ◽  
...  
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Embryo Development ◽  
Culture Medium ◽  
Essential Amino Acids ◽  
Human Embryos ◽  
Data Set ◽  
Group A ◽  
Amino Acids Profile ◽  
Group D

Abstract Study question How to determine human embryo viability noninvasively before embryo transfer? Summary answer We propose that the combination of the amino acid profile of an individual embryo with its morphokinetics will provide noninvasive tool to determine its viability. What is known already It was already known that human embryos at early cleavage require non-essential amino acids, while at the 8-cell to blastocyst stages, a mixture of non-essential and essential amino acids. Amino acids have important roles during embryo development. Acting as biosynthetic precursors,buffers of intracellular pH in the embryo, antioxidants, energy sources and regulators of metabolic function and signaling pathways. Many studies have used time-lapse to analyze human embryonic development including the process of fertilization and assessment of early events and introducednoninvasive prognostic markers which predict embryo development and correlate it to IVF treatment outcomes. Study design, size, duration This study was a prospective cohort study approved by the Clinical Trial Ethical Committee of Faculty of Medicine, Alexandria University according to ethical standards of scientific research (Serial number: 0303721).Thirty females aged 30.13 ± 4.83 years undergoing ICSI cycle in the Madina Fertility Center, during the period of March 2018 to November 2019.202 MII oocytes were incubated individually in embryoscope. Participants/materials, setting, methods Embryos (n = 161) were divided on Day 5 into two groups –developed embryos “Group D” (embryos that developed to blastocyst) and arrested embryos “Group A” (embryos remain at cleavage stage and fail to develop to blastocys).Developed embryos (Group D) included 99 embryos, and Arrested embryos (Group A) included 62 embryos. For each group, morphokinetic developmental points using embryoscope and the different amino acids concentrations in spent culture medium were analyzed using LC- mass spectro etry. Main results and the role of chance On one hand, the first appearance of pronuclei (TPNa), t2, t4 and CC2 in group D occurred significantly earlier than those of Group A.Analysis of 19 essential and non-essential amino acids in spent culture medium of each embryo in the two studied groups D and A showed a significantly higher concentration of two essential amino acids L-Valine (145.73 ± 150.96) and L-Phenylalanine (61.59 ± 55.78) in Group D than their concentration in Group A ( 104.58 ± 33.58, 44.24 ± 14.61, respectively , p ≤ 0.05).and significantly lower concentration of three non-essential amino acids L-Tyrosine (62.56 ± 41.03) , L-Cysteine (19.48 ± 11.90), and L-Alanine (136.0 ± 389.83) observed in Group D when compared to Group A (69.57 ± 20.78, 22.37 ± 8.59,145.33 ± 165.22, respectively, Limitations, reasons for caution It is important to note, that results were developed on a data set from one clinic with different stimulation protocols, a multicenter data and a correlation with the stimulation protocol used should be involved in future studies, in addition a larger sample size to avoid high standard deviation is recommended Wider implications of the findings: We can conclude that amino acid turnover is independent of the traditional morphological assessment of embryos and it may reflect its viability. The prospective combined use of amino acids profile of individual embryo and its morphokinetic parameters may contribute to introduce a new noninvasivs tool that may improve implantation rate Trial registration number 0303721

scholarly journals 46 How far we could go reducing crude protein with the use of supplemental amino acids

2019 ◽  
Vol 97 (Supplement_2) ◽  
pp. 22-23
Author(s):  
Candido Pomar
Keyword(s):  
Amino Acids ◽  
Dietary Protein ◽  
Optimal Growth ◽  
Essential Amino Acids ◽  
Growing Pigs ◽  
Growth Stages ◽  
Body Protein ◽  
Low Protein ◽  
Maintenance Requirements ◽  
Protein Reduction

Abstract Feeding growing pigs with diets providing the required amount of essential and non-essential amino acids (AA) reduces energy expenditure and minimizes N excretion. Low protein diets can be obtained by supplementing feeds with crystalline AA. Numerous experiments have evaluated the ideal dietary AA concentration at different growth stages, but reducing dietary protein with the use of supplemental AA is limited by the inaccuracy of the principles used to estimate AA requirements. One of these principles states that growing animals need AA for maintenance and growth. Maintenance requirements are related to BW whereas the efficiency of AA utilization (e.g., 72% for Lys) and body protein AA composition are constant (e.g., 7% for Lys). These parameters are, however, affected by AA restriction, meal frequency, energy supply, genetics, etc. Even when controlling these factors, individual pigs respond differently to the same AA supply. Yet pigs are raised in groups and fed with a unique feed for long periods. Individual pigs within a given population differ in terms of BW, ADG, health status, etc., and consequently, differ in the amount of AA they need at a given time. Therefore, when feeding a group of pigs, the concept of maintenance and growth requirements may not be appropriate. In this situation, nutrient requirements should be seen as the optimal balance between the proportion of animals that needs to be overfed and underfed. Given that for most AA, underfed animals exhibit reduced performance, whereas overfed animals exhibit near-optimal performance, optimal growth is obtained when nutrients are provided to satisfy the requirements of the most demanding animals. There is therefore a trade-off between performance and dietary protein reduction. The inaccuracy of the principles used to estimate AA requirements, both for individual animals and populations, limits how far we can go reducing dietary protein with the use of supplemental AA.

Plasma kinetics of essential amino acids following their ingestion as free formula or as dietary protein components

2016 ◽  
Vol 29 (4) ◽  
pp. 801-805 ◽  
Author(s):  
Mariangela Rondanelli ◽  
Roberto Aquilani ◽  
Manuela Verri ◽  
Federica Boschi ◽  
Evasio Pasini ◽  
...  
Keyword(s):  
Amino Acids ◽  
Dietary Protein ◽  
Essential Amino Acids ◽  
Plasma Kinetics ◽  
Protein Components ◽  
Kinetics Of

scholarly journals Restriction of essential amino acids dictates the systemic response to dietary protein dilution

2019 ◽  
Author(s):  
Yann W. Yap ◽  
Patricia M. Rusu ◽  
Andrea Y. Chan ◽  
Barbara C. Fam ◽  
Andreas Jungmann ◽  
...  
Keyword(s):  
Amino Acids ◽  
Dietary Protein ◽  
Metabolic Response ◽  
Essential Amino Acids ◽  
Metabolic Effects ◽  
Fibroblast Growth Factor 21 ◽  
Metabolic Dysfunction ◽  
Systemic Response ◽  
Transport Loss ◽  
Mice Liver

AbstractDietary protein dilution (DPD) promotes metabolic remodelling and health but the precise nutritional components driving this response remain elusive. Here we demonstrate that dietary amino acids (AA) are sufficient and necessary to drive the response to DPD. In particular, the restriction of dietary essential AA (EAA) supply, but not non-EAA, drives the systemic metabolic response to total AA deprivation. Furthermore, systemic deprivation of Thr and Trp, independent of total AA supply, are both adequate and necessary to confer the systemic metabolic response to both diet, and genetic AA-transport loss, driven AA restriction. Thr is also potentially limiting in low-protein diet fed humans, and dietary Thr restriction (DTR) retarded the development of obesity-associated metabolic dysfunction in mice. Liver-derived fibroblast growth factor 21 was required for the metabolic remodelling with DTR. Strikingly, hepatocyte-selective establishment of Thr biosynthetic capacity reversed the systemic response to DTR. Taken together, our studies demonstrate that the restriction of EAA are sufficient and necessary to confer the systemic metabolic effects of DPD.

THE EFFECT OF THE ESSENTIAL TO NONESSENTIAL AMINO ACID RATIO ON TURKEY PERFORMANCE AND CARCASS COMPOSITION

1988 ◽  
Vol 68 (3) ◽  
pp. 899-906 ◽  
Author(s):  
M. R. BEDFORD ◽  
J. D. SUMMERS
Keyword(s):  
Amino Acids ◽  
Weight Gain ◽  
Feed Intake ◽  
Dietary Protein ◽  
Total Protein ◽  
Protein Level ◽  
Essential Amino Acids ◽  
Carcass Composition ◽  
Feed Conversion ◽  
Total Protein Intake

The effect of altering the proportion of dietary protein supplied as essential amino acids (EAA) on the performance and carcass characteristics of young turkeys (to 3 wk of age) was investigated at three dietary protein levels (22, 26 and 30%) using semipurified diets. Four ratios of EAA to nonessential amino acids (NEAA) were employed (70:30, 60:40, 50:50 and 40:60) at each dietary protein level. Maxima in weight gain, percent and total carcass protein and feed intake were observed when EAA supplied 60% of total protein (i.e., the 60:40 ratio) at each protein level. The differences in weight gain and total carcass protein were largely attributed to differences in feed intake, since feed conversion efficiency was relatively unaffected by altering the EAA: NEAA ratio. More specifically, weight gain was shown to be limited by total protein intake. Since weight gain was maximized at the 60:40 ratio diets (in which all EAA are supplied at 128% of requirement) at least one of the EAA requirement values is incorrect. Key words: Turkeys, essential amino acids, protein requirement

The relationship between protein, essential amino acids and energy requirements of broiler chickens fed practical diets

1977 ◽  
Vol 17 (89) ◽  
pp. 920 ◽  
Author(s):  
N Guirguis
Keyword(s):  
Amino Acids ◽  
Protein Content ◽  
Dietary Protein ◽  
Broiler Chickens ◽  
Essential Amino Acids ◽  
Energy Requirements ◽  
Broiler Chicks ◽  
Maximal Growth ◽  
Dietary Concentration ◽  
The Relationship

The relationship between dietary protein, essential amino acids (EM) and energy was examined in two experiments using male broiler chicks fed practical diets to eight weeks of age. All diets contained a balanced proportion of ten EAA which was maintained over various treatments. Growth of birds was closely related to the EAA content of the diet and poorly related to dietary protein content. When the dietary concentration of ten EAA was treated as a combined unit and expressed as proportion of diet, 7.6 per cent EAA in an 18 per cent protein starter diet and 5.8 per cent EAA in a 16 per cent protein finisher diet were adequate to support maximal growth of male birds to eight weeks of age. Feed intakes were reduced as energy concentrations increased in diets regardless of protein content of diets.

scholarly journals Free amino acids in Viola tricolor in relation to different habitat conditions

2018 ◽  
Vol 16 (1) ◽  
pp. 833-841 ◽  
Author(s):  
Magdalena Dziągwa-Becker ◽  
Ryszard Weber ◽  
Olga Zajączkowska ◽  
Wiesław Oleszek
Keyword(s):  
Amino Acids ◽  
Aspartic Acid ◽  
Free Amino Acids ◽  
Free Amino ◽  
Essential Amino Acids ◽  
Habitat Conditions ◽  
Triple Quadrupole Mass Spectrometer ◽  
Viola Tricolor ◽  
Amino Acids Profile ◽  
And Cluster Analysis

AbstractThe purpose of this study was to establish the free amino acids profile of Viola tricolor collected from different habitats in Poland. Viola tricolor (heartsease) is a very popular plant found worldwide, classified both as weed and medicinal plant. Based on a validated method, the following nineteen free amino acids were analyzed using liquid chromatography-electrospray ionization coupled to a triple quadrupole mass spectrometer (LC-ESI-MS/MS):alanine, glycine, leucine, valine, isoleucine, proline, phenylalanine, tryptophan, tyrosine, serine, threonine, methionine, asparagine, glutamine, lysine, arginine, histidine, aspartic acid, glutamic acid. The total free amino acids (TAA) ranged from 9938.0 to 11393.8 mg/kg of fresh weight. The variability of the investigated amino acids with respect to different habitat conditions was statistically assessed using the method of discriminant and cluster analysis. Alanine, valine, glutamine and aspartic acid were the most abundant free amino acids present in both localizations. The ratio of total essential amino acids (EAA) to TAA was 0.27 and 0.11 in Zagródki and Wrocław, respectively. Discriminant analysis has demonstrated that the investigated habitats significantly differentiated the free amino acids content of Viola tricolor. Only methionine showed a similar concentration in both Viola tricolor populations.

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