scholarly journals Decreased metabolism and increased tolerance to extreme environments in Staphylococcus warneri during long‐term spaceflight

2019 ◽  
Vol 8 (12) ◽  
Author(s):  
Po Bai ◽  
Bin Zhang ◽  
Xian Zhao ◽  
Diangeng Li ◽  
Yi Yu ◽  
...  
Author(s):  
Abhinav Prasad ◽  
Karl Toland ◽  
Andreas Noack ◽  
Kristian Anastasiou ◽  
Richard Middlemiss ◽  
...  

<p><strong>Novelty / Progress Claim(s)</strong></p><p>This paper reports a capacitive readout-based MEMS relative gravimeter which can detect sub-Hz microseismic and slowly varying gravitational Earth tide signals. The gravimeter has a noise floor of 6-7 uGal/rt(Hz) at 1Hz and a linear drift of <250 uGal/day, metrics which are on a par with the commercially available gravimeters, and are leading in the field of MEMS accelerometers. The gravimeter is packaged in a standard ceramic-carrier and interfaced to a low-power, advanced FPGA-based readout. This setup is housed within a bespoke thermal enclosure, making the platform ideal for multi-pixel array-based implementation in the field.</p><p><strong>Background/State-of-the-Art</strong></p><p>Gravimeters are used to measure the local acceleration due to gravity (g). One of the emerging applications of gravimetry is in volcanology where gravimeters can be used to understand magma plumbing, providing information on volcanic activity/unrest events. However, this requires multi-pixel ‘gravity-imaging’ around volcanoes, a feat which is not possible using the expensive, complex, and large form-factor commercially available gravimeters.</p><p>Recently, researchers have developed MEMS-scale accelerometers which have excellent sensitivities but not yet demonstrated good long-term stability, making them non-viable for long-term monitoring of slow gravity changes (such as produced by magma flow). In a previous work, the authors have demonstrated an optical shadow-sensor readout based MEMS gravimeter with a sensitivity of 40 uGal/rt(Hz). Building on the work, a portable version of the gravimeter was also reported previously. The devices in both the setups were limited by the displacement noise of the optical shadow-sensor and the packageability of the setup.</p><p>In this paper, we are reporting a novel gravimeter which uses a capacitive-readout for sensing the proof-mass displacement, is embedded in a MEMS IC package, and uses advanced FPGA-based electronics for signal conditioning. The improved displacement sensitivity of the capacitive readout allows designing stiffer suspension-springs making the device more robust for operations in extreme environments. The acceleration sensitivity achieved using the new gravimeter is around 6-7 uGal/rt(Hz) at 1Hz, which is a significant improvement over the previous versions of the gravimeter. The device is currently being readied for field trials in the sectors of volcano gravimetry and oil & gas, showing the maturity of the technology.</p><p><strong>Methodology</strong></p><p>The reported gravimeter has a microfabricated silicon proof-mass which is suspended from thin flexures. Metal-combs are patterned on top of the proof-mass and a fixed glass layer with complementary combs is assembled to be at a close separation from the proof-mass. The overlapping combs act as a capacitor, the magnitude of which is dependent on the proof-mass displacement. The multi-layered gravimeter is embedded within a standard 32-pin ceramic DIP chip-carrier and wire bonded. The MEMS package is interfaced with analog signal conditioning electronics and a digital lock-in implementation is employed for converting the capacitance change into useful units (uGals).The electronics noise of the setup is measured to be <1 uGal. To reduce temperature-related effects, a mK active temperature control is implemented around the device. The packaged device is housed within a prototype thermal enclosure making the platform field-portable.</p>


2017 ◽  
Vol 49 (3) ◽  
pp. 269-274 ◽  
Author(s):  
Johan ASPLUND ◽  
Andy SIEGENTHALER ◽  
Yngvar GAUSLAA

AbstractLichens as sessile and slow-growing symbiotic associations have evolved various carbon-based secondary compounds (CBSCs) to mitigate the effects of some stressors in the extreme environments in which they often grow. The mat-forming lichen Cladonia stellaris, an important fodder for reindeer, produces usnic acid in the outermost layer and perlatolic acid in the medulla. Here, we studied effects of simulated global warming on these CBSCs in C. stellaris cultivated in climate chambers with: 1) ambient conditions as control or 2) ambient conditions +4°C. The chambers simulated, at an hourly resolution, an averaged 10-year growing season dynamic from a long-term monitored boreal mire in northern Sweden. After two months of acclimation, +4°C warming in one simulated growing season increased the concentration of usnic acid by 31% compared with ambient conditions, whereas the warming decreased the concentration of perlatolic acid by 14%. Since lichen CBSCs play important roles in ecosystem processes such as lichenivory and decomposition, these changes may profoundly affect lichen-dominated ecosystems.


Author(s):  
Andrea Securo ◽  
Emanuele Forte ◽  
Davide Martinucci ◽  
Simone Pillon ◽  
Renato R Colucci

This study investigates the application of a terrestrial structure from motionmulti-view stereo (SfM-MVS) approach combined with ground-penetrating radar (GPR) surveys for monitoring the surface topographic change of two permanent ice deposits in caves located in the Julian Alps (south-eastern European Alps). This method allows accurate calculation of both seasonal and annual mass balance, estimating the amount of ice inside caves. The ground-based SfM approach represents a low-cost workflow with very limited logistical problems of transportation and human resources and a fast acquisition time, all key factors in such extreme environments. Under optimal conditions, SfM-MVS allows sub-centimetric resolution results, comparable to more expensive and logistically demanding surveys such as terrestrial laser scanning (TLS). Fourteen SfM acquisitions were made between the 2017–2020 ablation seasons (i.e. July–October) while 2 GPR surveys were acquired in 2012. The obtained dense point clouds and digital terrain models (DTMs) made possible a reliable calculation of topographic changes and mass balance rates during the analysed period. The integration of SfM-MVS products with GPR surveys provided comprehensive imaging of the ice thickness and the total ice volume present in each of the caves, proving to be a reliable, low cost and multipurpose methodology ideal for long-term monitoring.


2018 ◽  
Vol 2 (4) ◽  
pp. 459-468 ◽  
Author(s):  
Corinna Bang ◽  
Ruth A. Schmitz

Archaea, the third domain of life containing unique membrane composition and highly diverse cell wall structures, were only recognized 40 years ago. Initially identified in extreme environments, they are currently recognized as organisms ubiquitously present in most, if not all, microbiomes associated with eukaryotic hosts. However, they have been mostly overseen in microbiome studies due to the lack of standardized detection protocols and to the fact that no archaeal pathogen is currently known. Recent years clearly showed that (i) archaea are part of the microbiomes associated with plants, animals and humans, (ii) form biofilms and (iii) interact and activate the human immune system. Future studies will not only define the host-associated diversity of archaea (referred to as ‘archaeome’) but also contribute to our understanding of the comprehensive metabolic interplay between archaea and bacteria and the long-term gain insights into their role in human health and their potential role(s) during disease development.


2019 ◽  
Vol 13 (2) ◽  
pp. 157-164
Author(s):  
Juntao Zhu ◽  
Yangjian Zhang ◽  
Wenfeng Wang ◽  
Xian Yang ◽  
Ning Chen ◽  
...  

Abstract Aims Grazing exerts profound effects on grassland ecosystem service and functions by regulating species composition and diversity, and structuring community assembly worldwide. However, adaptions of phylogenetic diversity and phylogenetic community structure to long-term grazing disturbance remain poorly studied, especially for ecosystems distributed in extreme environments. Methods Here, we conducted an experiment with multigrazing intensities to explore the impacts of grazing disturbance on plant phylogenetic diversity and community structure in an alpine grassland of the Tibetan Plateau. Important Findings Grazing disturbance enriched plant species richness (SR), and stimulated species turnover from regional species pool, consequently changing community species composition. Under low intensities, grazing exerted no obvious effects on phylogenetic diversity and community structure, whereas communities changed from overdispersion to clustering under high grazing intensity. High grazing intensity resulted in stronger environmental filtering, which consequently selected those species with high resilience to grazing disturbance. The observed clustering structure was associated with the colonizing species which were closely related to resident species, and locally extinct species, and distantly related to residents. At the plant functional trait level, high grazing intensity increased species colonization largely by altering the effect of root depth on species colonization compared to light grazing. Our results highlight that solely utilization of SR and diversity cannot fully represent grassland communities responses to grazing. The effects of species turnover on community phylogenetic diversity and structure are entailed to be explored in the future grazing studies.


2018 ◽  
Author(s):  
Po Bai ◽  
Bin Zhang ◽  
Xian Zhao ◽  
Diangeng Li ◽  
Yi Yu ◽  
...  

Background:Many studies have shown that space environment can affect bacteria to cause a range of mutations. However, so far, there are less studies on the effects of long-term space flight (>1 month) on bacteria. In this study, a Staphylococcus warneri strain, which was isolated from the Shenzhou-10 spacecraft that had experienced a space flight (15-day), was carried into space again. After 64-day flight, the combined phenotypic, genomic, transcriptomic and proteomic analyses were performed to compare the influence of the two space flights on this bacteria. Results: Compared with short-term space flight, long-term space flight increased the biofilm formation ability of S. warneri and the cell wall resistance to external environment stress, but reduced the sensitivity to chemical stimulation. Further analysis showed that these changes might be related to the significantly up-regulated gene expression of phosphotransferase system, which regulated glucose metabolism pathway, including glucose, mannose, fructose and cellobiose. The mutation of S. warneri caused by 15-day space flight was limited at phenotype and gene level after ground culture. Conclusion: After a 79-day space flight, the changes of S. warneri are meaningful. Phosphotransferase system of S. warneri was up-regulated by long-term space stimulation, which resulted in a series of changes in cell wall, biofilm and chemical sensitivity, thus enhancing the resistance and adaptability of bacteria to the external environment.


2021 ◽  
Author(s):  
Param Priya Singh ◽  
G. Adam Reeves ◽  
Kevin Contrepois ◽  
Mathew Ellenberger ◽  
Chi-Kuo Hu ◽  
...  

Suspended animation states such as hibernation or diapause allow organisms to survive extreme environments. But the mechanisms underlying the evolution of these extreme survival states are unknown. The African turquoise killifish has evolved diapause as a form of suspended development to survive the complete drought that occurs every year in its habitat. Here we show that many gene duplicates - paralogs - exhibit specialized expression in diapause versus normal development in the African turquoise killifish. Surprisingly, paralogs with specialized expression in diapause are evolutionarily very ancient, and they are also present even in vertebrates that do not exhibit diapause. Profiling the chromatin accessibility landscape among different fish species reveals an evolutionarily recent increase in chromatin accessibility at these very ancient paralogs, suggesting rewiring of their regulatory landscape. The increase in chromatin accessibility in the African turquoise killifish is linked to the presence of new binding sites for transcription factors (e.g., FOXO, REST, and PPAR), due to both de novo mutations and transposable element insertion. Interestingly, accessible chromatin regions in diapause are enriched for lipid metabolism genes. By performing lipidomics in different fish species, we uncover a specific lipid profile in African turquoise killifish embryos in diapause. Notably, select very long-chain fatty acids are high in diapause, suggesting they may be used for long-term survival in this state. Together, our multi-omic analysis indicates that diapause is driven by regulatory innovation of very ancient gene programs that are critical for survival. Our work also suggests a mechanism for how complex adaptations evolve in nature and offers strategies by which a suspended animation program could be reactivated in other species for long-term preservation.


2020 ◽  
Vol 1004 ◽  
pp. 1057-1065
Author(s):  
Philip G. Neudeck ◽  
David J. Spry

The prospects for beneficial application of integrated circuit (IC) capabilities in ambient environments above 450 °C have been significantly improved by recent long-term demonstrations of SiC chips and packaging by NASA Glenn Research Center. This invited paper reviews and updates development of durable SiC IC technology aspects relevant to engineering infusion into beneficial applications, including the first long-duration low-mass Venus lander missions


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