Properties of the tibialis anterior muscle after treatment with laser therapy and natural latex protein following sciatic nerve crush

Myosin heavy chain (MHC) expression was studied in rat soleus and tibialis anterior (TA) at the mRNA and protein levels following reinnervation 8 and 32 wk after sciatic nerve injury. A sciatic nerve crush or transection injury was produced in the midthigh region of adult female Sprague-Dawley rats. A ribonuclease protection assay was developed to measure four of the adult MHCs (I, IIa, IIx, IIb) in a single sample. MHC mRNA and protein were measured and compared in the same muscles. Eight and thirty-two weeks after a crush injury, the MHC mRNA profiles were similar to control with the exception of soleus MHC IIa and TA MHC IIb, which were significantly less than control at both time points. In contrast, reinnervation of the soleus following a sciatic nerve transection injury resulted in an MHC isoform shift characterized by increases in the relative amounts of fast myosin (IIa and IIx) and a decrease in slow myosin. As expected, significant changes first occurred at the mRNA level followed by changes in protein expression. Thirty-two weeks after transection injury and repair, the primary MHC mRNA isoform in the soleus was MHC IIx. Moreover, at 32 wk, MHC IIb mRNA was detected in 50% of the reinnervated soleus following a transection injury. Reinnervation of the TA following sciatic nerve transection led to replacement of the MHC IIb isoform with MHC IIx.

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Muscle-derived GDNF facilitates motor nerve recovery following sciatic nerve crush injury

Journal of the American College of Surgeons ◽

10.1016/j.jamcollsurg.2007.06.230 ◽

2007 ◽

Vol 205 (3) ◽

pp. S92

Author(s):

Terence M. Myckatyn ◽

Christina Kenney ◽

Alice Tong ◽

Jessica Duan ◽

Daniel Hunter ◽

...

Keyword(s):

Sciatic Nerve ◽

Motor Nerve ◽

Crush Injury ◽

Sciatic Nerve Crush ◽

Nerve Crush ◽

Nerve Recovery ◽

Nerve Crush Injury

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Myelinated fiber regeneration after sciatic nerve crush: Morphometric observations in young adult and aging mice and the effects of macrophage suppression and conditioning lesions

Experimental Neurology ◽

10.1016/0014-4886(92)90022-i ◽

1992 ◽

Vol 118 (1) ◽

pp. 53-61 ◽

Cited By ~ 61

Author(s):

Kaoru Tanaka ◽

Qian-Lin Zhang ◽

Henry deF. Webster

Keyword(s):

Young Adult ◽

Sciatic Nerve ◽

Sciatic Nerve Crush ◽

Myelinated Fiber ◽

Nerve Crush

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Functional Recovery and Vitamin E Level Following Sciatic Nerve Crush Injury in Normal and Diabetic Rats

International Journal of Neuroscience ◽

10.3109/00207459808986472 ◽

1998 ◽

Vol 96 (3-4) ◽

pp. 245-254 ◽

Cited By ~ 10

Author(s):

Khalaf Al Moutaery ◽

Mohammed Arshaduddin ◽

Mohammad Tariq ◽

Saleh Al Deeb

Keyword(s):

Vitamin E ◽

Sciatic Nerve ◽

Functional Recovery ◽

Diabetic Rats ◽

Crush Injury ◽

Sciatic Nerve Crush ◽

Nerve Crush ◽

Nerve Crush Injury

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Canine Adipose-Derived Mesenchymal Stromal Cells Enhance Neuroregeneration in a Rat Model of Sciatic Nerve Crush Injury

Cell Transplantation ◽

10.1177/0963689718809045 ◽

2018 ◽

Vol 28 (1) ◽

pp. 47-54 ◽

Cited By ~ 7

Author(s):

Diego Noé Rodríguez Sánchez ◽

Luiz Antonio de Lima Resende ◽

Giovana Boff Araujo Pinto ◽

Ana Lívia de Carvalho Bovolato ◽

Fábio Sossai Possebon ◽

...

Keyword(s):

Sciatic Nerve ◽

Mesenchymal Stromal Cells ◽

Rat Model ◽

Stromal Cells ◽

Multipotent Mesenchymal Stromal Cells ◽

Crush Injury ◽

Sciatic Nerve Crush ◽

Muscle Weight ◽

Nerve Crush ◽

Nerve Crush Injury

Crush injuries in peripheral nerves are frequent and induce long-term disability with motor and sensory deficits. Due to axonal and myelin sheath disruptions, strategies for optimized axonal regeneration are needed. Multipotent mesenchymal stromal cells (MSC) are promising because of their anti-inflammatory properties and secretion of neurotrophins. The present study investigated the effect of canine adipose tissue MSC (Ad-MSC) transplantation in an experimental sciatic nerve crush injury. Wistar rats were divided into three groups: sham ( n = 8); Crush+PBS ( n = 8); Crush+MSC ( n = 8). Measurements of sciatic nerve functional index (SFI), muscle mass, and electromyography (EMG) were performed. Canine Ad-MSC showed mesodermal characteristics (CD34-, CD45-, CD44+, CD90+ and CD105+) and multipotentiality due to chondrogenic, adipogenic, and osteogenic differentiation. SFI during weeks 3 and 4 was significantly higher in the Crush+MSC group ( p < 0.001). During week 4, the EMG latency in the Crush+MSC groups had better near normality ( p < 0.05). The EMG amplitude showed results close to normality during week 4 in the Crush+MSC group ( p < 0.04). There were no statistical differences in muscle weight between the groups ( p > 0.05), but there was a tendency toward weight gain in the Crush+MSC groups. Better motor functional recovery after crush and perineural canine Ad-MSC transplantation was observed during week 2. This was maintained till week 4. In conclusion, the canine Ad-MSC transplantation showed early pro-regenerative effects between 2–4 weeks in the rat model of sciatic nerve crush injury.

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