Down-regulation of dishevelled-2 inhibits cell proliferation and invasion in hepatoblastoma

2018 ◽  
Vol 65 (7) ◽  
pp. e27032 ◽  
Author(s):  
Siqi Huang ◽  
Jing Chen ◽  
Ruicheng Tian ◽  
Jing Wang ◽  
Chenjie Xie ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Down Regulation ◽  
Proliferation And Invasion

scholarly journals Down-regulation LncRNA-SNHG15 contributes to proliferation and invasion of bladder cancer cells

BMC Urology ◽  
2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Aldhabi Mokhtar ◽  
Chuize Kong ◽  
Zhe Zhang ◽  
Yan Du
Keyword(s):  
Cell Proliferation ◽  
Bladder Cancer ◽  
Cancer Cells ◽  
Cell Lines ◽  
Tumor Stage ◽  
Rt Pcr ◽  
Down Regulation ◽  
Bladder Cancer Cells ◽  
Cancer Tissues ◽  
Proliferation And Invasion

Abstract Objectives The aim of this study was to investigate the effect of lncRNA-SNHG15 in bladder carcinoma using cell lines experiments and the relationship between clinical characteristics and lncRNA-SNHG15 expression was analyzed. Methods Bladder cancer tissues and near-cancer tissues were collected. The real-time PCR (RT-PCR) was used to detect the expression of lncRNA-SNHG15 in tissues and cell lines. The expression of lncRNA-SNHG15 was downregulated by interference (siRNA), as detected by RT-PCR, that was used to determine the efficiency of the interference. CCK-8 and Transwell assays were used to evaluate the effect of lncRNA-SNHG15 on the proliferation and invasion capability of bladder cancer cells. The t-test was used for Statistical analyses, which were carried out using the Statistical Graph pad 8.0.1.224 software. Result The expression of lncRNA-SNHG15 was up regulated in 5637, UMUC3 and T24 cell lines compared with corresponding normal controls (P < 0.05). Up regulation was positively related to tumor stage (P = 0.015). And tumor size (P = 0.0465). The down-regulation of lncRNA-SNHG15 with siRNA significantly inhibited UMUC3 and T24 cell proliferation and invasion. Conclusion This study showed that lncRNA-SNHG15 is overexpressed in bladder cancer tissues and (5637, UMUC3 T24) cell lines. Up regulation was positively related to tumor stage (P = 0.015), and tumor size (P = 0.0465). Down-regulation of lncRNA-SNHG15 by siRNA significantly inhibited UMUC3 and T24 cell proliferation and invasion, indicating a potential molecular target for future tumor targeted therapy.

scholarly journals Hsa_circ_0026134 expression promoted TRIM25- and IGF2BP3-mediated hepatocellular carcinoma cell proliferation and invasion via sponging miR-127-5p

2020 ◽  
Vol 40 (7) ◽  
Author(s):  
Wei Zhang ◽  
Liang Zhu ◽  
Guowei Yang ◽  
Bo Zhou ◽  
Jianhua Wang ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Cell Proliferation ◽  
Cell Lines ◽  
Luciferase Reporter ◽  
Circular Rnas ◽  
Down Regulation ◽  
Mrna Binding Protein ◽  
New Treatment ◽  
Mrna Binding ◽  
Proliferation And Invasion

Abstract Increasing evidence shows that circular RNAs (circRNAs) play a regulatory role in cancer. In the present study, we aimed to investigate the characteristics and effects of hsa_circ_0026134 in hepatocellular carcinoma (HCC). We investigated hsa_circ_0026134 expression in 20 pairs of clinical tissues from HCC patients; expression of hsa_circ_0026134 in different cell lines; effect of hsa_circ_0026134 on proliferation and invasion of HCC cell lines; and the regulatory mechanisms and interactions among hsa_circ_0026134, miR-127-5p, tripartite motif-containing protein 25 (TRIM25) and insulin-like growth factor 2 mRNA-binding protein 3 (IGF2BP3). hsa_circ_0026134 expression was increased in HCC samples and cell lines. Down-regulation of hsa_circ_0026134 attenuated HCC cell proliferation and metastatic properties. Micro (mi)RNA (miR)-127-5p was sponged by hsa_circ_0026134. Rescue experiments indicated that inhibition of miR-127-5p expression promoted cell proliferation and invasion even after hsa_circ_0026134 silencing. TRIM25 and IGF2BP3 were targets of miR-127-5p. Overexpression of TRIM25 or IGF2BP3 promoted cell proliferation and invasion in cells overexpressing miR-127-5p. Down-regulation of hsa_circ_0026134 suppressed TRIM25- and IGF2BP3-mediated HCC cell proliferation and invasion via promotion of miR-127-5p expression, which have been confirmed by luciferase reporter assay. The present study provides a new treatment target for HCC.

scholarly journals Down-regulation of USP8 Inhibits Cholangiocarcinoma Cell Proliferation and Invasion

2020 ◽  
Vol Volume 12 ◽  
pp. 2185-2194
Author(s):  
Xu Jing ◽  
Yingjie Chen ◽  
Ye Chen ◽  
Guangyan Shi ◽  
Shuanghao Lv ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Down Regulation ◽  
Cholangiocarcinoma Cell ◽  
Proliferation And Invasion

Abstract 187: Down-regulation of microRNA34 induces cell proliferation and invasion of human mesothelial cells

2012 ◽  
Author(s):  
Norimitsu Tanaka ◽  
Shinichi Toyooka ◽  
junichi soh ◽  
Kazunori Tsukuda ◽  
Kazuhiko Shien ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Mesothelial Cells ◽  
Down Regulation ◽  
Proliferation And Invasion

Silencing of the hPOT1 gene by RNA inference promotes apoptosis and inhibits proliferation and aggressive phenotype of gastric cancer cells, likely through up-regulating PinX1 expression

2011 ◽  
Vol 64 (12) ◽  
pp. 1051-1057 ◽  
Author(s):  
Shun-Mei Wan ◽  
Jun Tie ◽  
Ya-Fei Zhang ◽  
Jun Guo ◽  
Liu-Qin Yang ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Cell Proliferation ◽  
Intestinal Metaplasia ◽  
Cancer Cells ◽  
Protein A ◽  
Normal Mucosa ◽  
Gastric Cancer Cells ◽  
Down Regulation ◽  
Proliferation And Invasion

BackgroundThe human protection of telomeres 1 (hPOT1) protein, a single-strand telomeric DNA binding protein, plays an important role in telomere protection and telomere length regulation. However, its effect on invasion of gastric cancer remains unclear.AimsTo explore the role of hPOT1 in the proliferation and invasion of gastric cancer cells.MethodsThe gastric expression of hPOT1 was examined in normal gastric mucosa (n=25), intestinal metaplasia (n=20), gastric dysplasia (n=20) and gastric cancer (n=150) by immunohistochemistry. The mean optical density (MOD) of the immunostaining was determined by semi-quantitative image analysis. The role of hPOT1 in the cell proliferation, apoptosis and invasion of gastric cancer 7901 cells was determined by means of the RNA interference (RNAi) of hPOT1 mRNA. The effects of hPOT1 RNAi on the expression of hPinX1 and hTERT were detected with western blotting.ResultsThe hPOT1 MOD was progressively increased from the normal mucosa to intestinal metaplasia, dysplasia, and gastric cancer. An increased hPOT1 expression significantly correlated with tumour serosal invasion, node metastasis and advanced stage. Transfection of hPOT1 siRNA into SGC-7901 cells led to a decrease in cell proliferation, colony formation and invasion, and also an increase of apoptosis. An up-regulation of hPinX1 and down-regulation of hTERT were found in gastric cancer cells with hPOT1 siRNA.ConclusionsIncreased hPOT1 expression is associated with an advanced tumour stage. hPOT1 RNAi inhibits proliferation and invasion, and induces apoptosis of gastric cancer cells. The effects of hPOT1 RNAi seem to be functionally linked to up-regulation of PinX1 and down-regulation of hTERT.

Down-regulation LncRNA-SNHG15 Contributes to Proliferation and Invasion of Bladder Cancer Cells

2021 ◽  
Author(s):  
Aldhabi Mokhtar ◽  
Chuize Kong ◽  
Zhe Zhang ◽  
Yan Du
Keyword(s):  
Cell Proliferation ◽  
Bladder Cancer ◽  
Cell Lines ◽  
Tumor Size ◽  
Tumor Stage ◽  
Rt Pcr ◽  
Down Regulation ◽  
Statistical Graph ◽  
Cancer Tissues ◽  
Proliferation And Invasion

Abstract OBJECTIVES: This study aimed to investigate effect of lncRNA-SNHG15 in bladder carcinoma using cell lines experiments and the relationship between clinical characteristics and lncRNA-SNHG15 expression was analyzed.Methods. Bladder cancer tissues and near-cancer tissues were collected. The expression of lncRNA-SNHG15 in tissues and cell lines was detected by real-time PCR (RT-PCR). The expression of lncRNA-SNHG15 was downregulated by interference (siRNA) as detected by RT-PCR that was used to detect the interference efficiency. CCK-8, and Transwell assays were used to evaluate the effect of lncRNA-SNHG15 on the proliferation, invasion capa­bility of bladder cancer cells.t-test was used for Statistical analyses, were performed using the Statistical Graph pad 8.0.1.224 software.Result: The expression of lncRNA-SNHG15 was up regulated in 5637, UMUC3 and T24 cell lines compared with corresponding normal controls (P<0.05). up regulation was positively related to tumor stage (P = 0.015), and tumor size (P =0.0465) . The down-regulation of lncRNA-SNHG15 with siRNA significantly inhibited UMUC3 and T24 cell proliferation and invasion.Conclusion: This study showed that lncRNA-SNHG15 is overexpressed in bladder cancer tissues and (5637, UMUC3 T24) cell lines. up regulation was positively related to tumor stage (P = 0.015), and tumor size (P =0.0465) .The down-regulation of lncRNA-SNHG15 with siRNA significantly inhibited UMUC3 and T24 cell proliferation and invasion, which provides a potential molecular target for future tumor targeted therapy.

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